Mineralized tissues accumulate elements that play crucial roles in animal health. Although elemental content of bone, blood and teeth human some species have been characterized, data for many others are lacking, as well comparisons. Here we describe the distribution horn (Bovidae), antler (Cervidae), bone (humerus) across a number determined by handheld X-ray fluorescence (XRF) to better understand differences potential biological relevance. A difference profiles between horns antlers was...

In our previous study, we identified an miRNA regulatory network involved in energy metabolism porcine muscle. To better understand the involvement of miRNAs cellular ATP production and metabolism, here used C2C12 myoblasts, which levels increase during differentiation, to identify modulating these processes. level, mRNA microarray expression profiles differentiation into myotubes were assessed. The results suggest 14 (miR-423-3p, miR-17, miR-130b, miR-301a/b, miR-345, miR-15a, miR-16a,...

Physiological processes aiding the conversion of muscle to meat involve many genes associated with structure and metabolic processes. MicroRNAs regulate networks orchestrate cellular functions, in turn regulating phenotypes.We applied weighted gene co-expression network analysis identify modules that correlated quality phenotypes were highly enriched for involved glucose metabolism, response wounding, mitochondrial ribosome, mitochondrion, extracellular matrix. Negative correlation miRNA...

Primary cultures from Asian elephants (Elephas maximus) allow scientists to obtain representative cells that have conserved most of their original characteristics, function, physiology and biochemistry. This technique has thus gained significant importance as a foundation for further cellular, cell biology molecular research. Therefore, the aim this study was describe conditions successful establishment primary adult fibroblasts elephant carcasses.Ear tissue sample collection carcasses our...

Abstract Canine babesiosis is a tick-borne disease caused by Babesia spp., which infects and destroys healthy erythrocytes, leading to mortality morbidity in dogs. The diagnosis of tedious time-consuming, especially latent chronic infections. Here, recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay was developed for rapid accurate detection spp. canine blood specimens based on the 18S rRNA region. RPA-LFD using rpaBab264 gave specificity dogs ( B. vogeli...

Understanding the genetic contributions behind skeletal muscle composition and metabolism is of great interest in medicine agriculture. Attempts to dissect these complex traits combine genome-wide genotyping, expression data analyses network analyses. Weighted gene co-expression analysis (WGCNA) groups genes into modules based on patterns co-expression, which can be linked phenotypes by correlation trait values module eigengenes, i.e. first principal component a given module. Network hub...

Objective: Environmental stress induces disturbances in cell energy metabolism and may cause epigenetic modifications. This study aimed to understand the possible impact of temperature (35 °C, 39 °C 41 compared control 37 °C) on modifications, such as DNA methylation histone H4 acetylation, well its effects expression genes responsible for changes, mouse skeletal myoblasts (C2C12 cells).Methods: The results showed significantly reduced maximal respiration spare respiratory capacity under...

Unraveling the intricate and tightly regulated process of adipogenesis, involving coordinated activation transcription factors signaling pathways, is essential for addressing obesity related metabolic disorders. The molecular pathways recruited by mesenchymal stem cells (MSCs) during adipogenesis are also dependent on different sources genetic backgrounds donors, which contribute to functional heterogeneity consequently affect developmental features fate cells.

MicroRNAs (miRNAs) and mRNAs establish a complex regulatory network influencing diverse biological pathways including muscle development growth. Elucidating miRNA-dependent networks involved in could provide additional insights into traits largely predefined during prenatal development. The present study aimed to determine differentially expressed transcripts functional miRNA–mRNA relationships associated with different stages of skeletal two pig breeds, German Landrace Pietrain, distinct...

Phosphorus is an essential mineral for all living organisms and a limited resource worldwide. Variation heritability of phosphorus utilization (PU) traits were observed, indicating the general possibility improvement. Molecular mechanisms PU, including host microbial effects, are still poorly understood. The most promising molecules that interact between microbiome microRNAs. Japanese quail representing extremes PU selected from F2 population miRNA profiling ileal tissue subsequent...

Polyunsaturated fatty acids (PUFAs) are the main components of cell membrane affecting its fluidity, signaling processes and play a vital role in muscle development. The effects docosahexaenoic acid (DHA) on myogenesis well known, while arachidonic (AA) largely unclear. purpose this study is to evaluate effect two PUFAs (DHA AA) fate during myogenic processes, Wnt energy metabolism by using C2C12 cells. cells were treated with different concentrations AA or DHA for 48 h differentiation...

Abstract Synovial membrane mesenchymal stem cells (SMSCs) often serve as in vitro model for bone disease, but the molecular mechanisms driving osteogenesis SMSCs from different donor of various sources and breeds remain unclear. In this study, porcine isolated adipose synovium (FP) fibrous (FS) Angeln Saddleback (AS) German Landrace (DL) were used to discover signaling network change after osteogenic induction. During differentiation, mineral deposition was first observed at day 14 further...

Intra-articular injection of corticosteroids is used to treat the inflammatory pain arthritis and osteoarthritis (OA), but our previous study found a deleterious effect these steroids on chondrocyte cells. Hyaluronic acid (HA) has been suggested as means counteract negative side effects through replenishment synovial fluid that can decrease in affected joints. To better understand processes, dexamethasone (Dex) prednisolone (Pred) were administered porcine cartilage explants at several...

Temperature stress is one of the main environmental stressors affecting welfare, health and productivity livestock. changes can modify cell membrane components, disrupting crosstalk between its surroundings by signaling pathways including Wnt pathway, which subsequently disrupts energy metabolism. The present study aims to understand effect temperature on expression genes involved in pathways, their interaction with metabolism C2C12 myoblasts cells. cells were exposed cold (35 °C), mild heat...

Fluoroquinolones ( FQ s) are frequently used for septic arthritis. Increased antibacterial activity has been associated with mammalian cell cytotoxicity that may increase the risk of developing osteoarthritis. This study compared direct effects two different s, enrofloxacin (Enro) and marbofloxacin (Mar), on normal primary canine chondrocytes inflammatory‐stimulated chondrocytes, in addition to their administration combination hyaluronan HA ). Cell viability, apoptosis, s‐ GAG production,...

The purpose of this study was to determine the effect bromelain treatment on canine articular chondrocytes in vitro. This research evaluated cell viability, levels apoptotis and mitotis, proteoglycan concentrations expression certain genes. Chondrocytes were exposed 50 μg/ml for 4, 16 32 h. rate groups significantly lower than control that incubated with media only (P < 0.05); mitotic higher 0.05), at all durations exposure. gene measured by real-time PCR technique. It found decreased 0.05)...