A5033101660- Adipose Tissue and Metabolism
- Meat and Animal Product Quality
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Fermentation and Sensory Analysis
- Cancer-related molecular mechanisms research
- Horticultural and Viticultural Research
- Muscle Physiology and Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Insect and Arachnid Ecology and Behavior
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Behavior and Welfare Studies
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Effects of Environmental Stressors on Livestock
- Food Quality and Safety Studies
- Plant biochemistry and biosynthesis
- Sirtuins and Resveratrol in Medicine
- Insect-Plant Interactions and Control
- Fungal Biology and Applications
- Plant and Fungal Interactions Research
- Hemiptera Insect Studies
- Antioxidant Activity and Oxidative Stress
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2006-2024
Consejo Superior de Investigaciones Científicas
2005-2023
University of California, Merced
2023
Smithsonian Tropical Research Institute
2023
Instituto Nacional de Investigación Agropecuaria
2021
Universidade da Coruña
2021
University of Chile
2019
Centro de Estudios Andaluces
2013
Unidades Centrales Científico-Técnicas
2012
Instituto de Investigación en Ciencias de la Alimentación
2011
Genetic characterization of local breeds is essential to preserve their genomic variability, advance conservation policies and contribute promotion sustainability. Genomic diversity twenty European pig a small sample Spanish wild pigs was assessed using high density SNP chips. A total 992 DNA samples were analyzed with the GeneSeek Profiler (GGP) 70 K HD porcine genotyping chip. Genotype data employed compute genetic diversity, population differentiation structure, distances, linkage...
The aim of this work was to analyse the distribution causal and candidate mutations associated relevant productive traits in twenty local European pig breeds. Also, potential SNP panel employed for elucidating genetic structure relationships among breeds evaluated. Most genes with morphological, productive, meat quality, reproductive disease resistance were prioritized analyzed a maximum 47 blood samples from each (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian...
Iberian ham production includes both purebred (IB) and Duroc-crossbred (IBxDU) pigs, which show important differences in meat quality traits, such as muscle growth fatness. This experiment was conducted to investigate gene expression differences, transcriptional regulation genetic polymorphisms that could be associated with the observed phenotypic between IB IBxDU pigs. Nine 10 pigs were slaughtered at birth. Morphometric measures blood samples obtained from Biceps femoris employed for...
The two main genetic types in Iberian pig production show important phenotypic differences growth, fattening and tissue composition since early developmental stages. objective of this work was the evaluation muscle transcriptome profile piglets both types, order to identify genes, pathways regulatory factors responsible for their differences. Contemporary families coming from pure pigs (IB) or crossing with Duroc boars (DU×IB) were generated. Piglets (14 each type) slaughtered at weaning (28...
Natural and artificial directional selection in cosmopolitan autochthonous pig breeds wild boars have shaped their genomes resulted a reservoir of animal genetic diversity. Signatures are the result these events that contributed to adaptation different environments production systems. In this study, we analysed genome variability 19 European (Alentejana, Bísara, Majorcan Black, Basque, Gascon, Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano, Sarda, Krškopolje pig,...
Five mutants (obtained by UV mutagenesis) and the parent strain were selected to produce sparkling wines following traditional or champenoise method. The aged with yeast for 9 months, samples being taken each month analytical sensory determinations. elaborated mutant IFI473I demonstrated an accelerated release of protein, amino acids, polysaccharides. An analysis secreted polysaccharides revealed that mannose was major sugar present. effects products released yeasts on foaming properties...
Two different yeast cell wall extracts were obtained using enzymatic digestion and thermal treatment. The effects of the on foaming properties a model wine two sparkling wines studied. wines, supplemented with extract, presented better than did samples extract. fractioning (Con A chromatography) characterization (SDS-PAGE, SEC, GC, RP-HPLC) both showed that fraction responsible for is constituted by mannoproteins relative molecular weight between 10 30 kDa, presenting an equilibrated...
Iberian pig production includes purebred (IB) and Duroc-crossbred (IBxDU) pigs, which show important differences in growth, fattening tissue composition. This experiment was conducted to investigate the effects of genetic type muscle (Longissimus dorsi (LD) vs Biceps femoris (BF)) on gene expression transcriptional regulation at two developmental stages. Nine IB 10 IBxDU piglets were slaughtered birth, seven four months age (growing period). Carcass traits LD intramuscular fat (IMF) content...
Meat quality depends on tissue composition which is in turn influenced by different factors, such as diet, genotype, age, or sex. We evaluated the effects of breed, 24 h fasting, and dietary energy source (HO: oleic acid versus CH: carbohydrates) expression candidate genes involved adipogenesis, lipogenesis, lipolysis adipose from Iberian Duroc growing pigs. The pigs showed greater feed intake, backfat thickness, saturated fatty acids (SFA) content subcutaneous fat, whereas had ham weight...
Abstract Coastal biota is exposed to continuous environmental variability as a consequence of natural and anthropogenic processes. Responding heterogeneous conditions requires the presence physiological strategies cope with environment. Ecosystems influenced by upwelling endure naturally cold, acidic hypoxic conditions, nevertheless they sustain major fisheries worldwide. This suggests that species inhabiting habitats possess adaptations handle high variability. Here, we assessed impact main...
Summary ROHs are long stretches of DNA homozygous at each polymorphic position. The proportion genome covered by and their length indicators the level origin inbreeding. Frequent common within same population define ROH islands indicate hotspots selection. In this work, we investigated in a total 1131 pigs from 20 European local pig breeds three cosmopolitan breeds, genotyped with GGP Porcine HD Genomic Profiler. plink software was used to identify ROHs. Size classes genomic inbreeding...
In this study, we analyzed the effects of breed, diet energy source, and their interaction on adipose tissue transcriptome in growing Iberian Duroc pigs. The study comprised 29 19 males, which were kept under identical management conditions except nutritional treatment. Two isoenergetic diets used with 6% high oleic sunflower oil (HO) or carbohydrates (CH) as sources. All animals slaughtered after 47 days treatment at an average live weight 51.2 kg. Twelve from each breed (six fed diet)...
The use of polyphenols is a promising strategy for preventing or alleviating intrauterine growth restriction (IUGR) because polyphenol supplementation increases plasma antioxidant capacity and improves oxidative stress at the feto-placental unit; which are recognized as main issues in IUGR. However, there scarcity experimental data on both realistic benefits potential hazards during gestation. Hence, we aimed to swine model IUGR pregnancy determine possible effects maternal with...
When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous intramuscular fat (IMF) deposition which contributes better meat quality. The aim of this work was explore the genome function understand underlying physiological mechanisms associated with body accretion. Dorsal samples were collected at slaughter from adult animals (n = 4 each breed) ~150 kg weight. Total RNA obtained sequenced transcriptome...
Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed explore transcriptomics Longissimus lumborum two local pig breeds with distinct genetic background using next-generation sequencing technology Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed Alentejano (AL) 15 Bísaro (BI) breed. Specific slow type myosin heavy chain...
Abstract Iberian pigs and its crosses are produced to obtain high-quality meat products. The objective of this work was evaluate a wide panel DNA markers, selected by biological functional criteria, for association with traits related muscle growth, fatness, quality metabolism. We used 18 crossbred divergent postnatal growth patterns whole genome sequencing SNP discovery, over 13 million variants being detected. 1023 missense SNPs located on annotated genes showing different allele...
A trial was performed to compare the effects of different dietary sources MUFA on fatty acid (FA) composition, lipid metabolism, and gene transcription in tissues Iberian pigs. Twenty-seven male pigs 28 kg live weight (LW) were divided 2 groups fed with 1 isocaloric diets: a standard diet carbohydrates as energy source (CH) enriched high-oleic sunflower oil (HO). Ham adipose tissue sampled by biopsy at 44 70 LW. At 110 LW slaughtered backfat, loin, liver sampled. Animals HO group showed...
Abstract: In the present work, 3 different yeast strains (1, 2, and 3) were used to elaborate white wines using Albariño must. The concentration of polymeric mannose was determined a method based on mannoprotein precipitation, hydrolysis analysis sylylated derivatives by gas chromatography. Wines elaborated with strain 1 (W1) presented higher than other wines. volatile composition showed significant differences ( P < 0.05) among them, being W1 which highest aroma compounds, mainly terpens...
RNA-Seq technology is widely used in quantitative gene expression studies and identification of non-annotated transcripts. However this also can be for polymorphism detection RNA editing transcribed regions an efficient cost-effective way. This study SNP data from assay to identify genes mutations underlying production trait variations experimental pig population. The hypothalamic hepatic transcriptomes nine extreme animals growth fatness (Iberian × Landrace) Landrace backcross were analyzed...
Summary In this study, we identified copy number variants (CNVs) in 19 European autochthonous pig breeds and two commercial (Italian Large White Italian Duroc) that represent important genetic resources for species. The genome of 725 pigs was sequenced using a breed‐specific DNA pooling approach (30–35 animals per pool) obtaining an average depth pool 42×. This maximised CNV discovery as well the related states characterising, on average, analysed breeds. By mining more than 17.5 billion...
The Iberian pig breed includes several well-differentiated strains. present study evaluated carcass traits, fat deposition and muscle expression of important lipogenic genes (SCD, ME1, ACACA, FASN, EGR1, ACOX ACLY) using 65 male pigs 3 strains (20 Lampiño, 23 Torbiscal, 22 Retinto) finished either in a conventional, concentrate-based system (CF) or montanera (MF), traditional free-range with acorn feeding. Torbiscal had the highest ham, Longissimus thoracis prime cuts yields, thinnest...