Tim-3 is a negative immunoregulator in anti-tumor response, but its mechanism chronic lymphocytic leukemia (CLL) not yet clear. The aim of this study was to understand the role Galectin-9/Tim-3 signaling pathway regulation CD4+ T cell subsets CLL patients. Flow cytometry results showed that number Treg cells obviously increased, and there significant Treg/Th17 imbalance In addition, overexpressed on surface Th1 levels Galectin-9 IL-10 were significantly elevated patients CLL, especially...

Abstract Background The fine balance of Th17/Treg is crucial for maintenance immune homeostasis. objective this study was to investigate the and expression related cytokines in Uighur cervical cancer patients. Methods Peripheral blood collected from 65 cases patients, 42 CIN patients 40 healthy people. Flow cytometry used detect percentages T cell subsets, including CD3 + cells, CD4 CD8 Treg cells Th17 cells. ELISA assay conducted levels TGF-β, IL-6, IL-10, IL-17, IL-23 IFN-γ. Results There...

This study is to investigate the role of regulatory B (Breg) cells in cervical cancer. In total, 70 cases cancer, 52 intraepithelial neoplasia (CIN), and 40 normal controls were enrolled. The percentage Breg was detected by flow cytometry. Serum levels IL-10 measured ELISA. correlation between clinical characterizations cancer analyzed. inhibition effect on CD8 + T tested blocking vitro . CD19 CD5 CD1d level patients with or CIN significantly higher than those control group (<mml:math...

Th9 cells have been reported to contribute immune responses; however, the role of in Echinococcus granulosus infection is unknown. This study determine whether and IL-9 are involved human infection. Compared with healthy controls (HC group), mRNA levels PU.1, IL-9, GATA-3 were significantly increased patients before therapy (CE as revealed by qRT-PCR. Flow cytometry analysis showed that percentages Th2 CE group higher. The IL-4, IL-10, TGF- β also increased, detected CBA assay. positively...

Abstract This study aims to investigate the changes of cytokines and effect programmed death ligand 1 (PD-L1) signaling pathway on T cell function in patients with immune thrombocytopenic purpura (ITP). Totally, 40 untreated ITP were recruited 30 healthy people as control. Then whole blood control was collected, respectively. The sPD-L1/anti-PD-1 used activate or block (PD-1)/PD-L1 pathway. expression PD-1 PD-L1 peripheral mononuclear cells (PBMCs) detected by flow cytometry. PBMCs treated...

The present study is to measure the expression of programmed death-1 (PD-1) and death ligand-1 (PD-L1), as well its clinical significance in cervical cancer patients. Our results showed that different T cell subsets patients with had high PD-1, DCs PD-L1. High PD-1 on Treg cells facilitated production TGF-β IL-10 but inhibited IFN-γ. Cervical elevated PD-L1 mRNA level. peripheral blood was related tumor differentiation, lymph node metastasis, invasiveness. PD-1/PD-L1 pathway lymphocyte...

Acute graft-versus-host disease (aGVHD) arises from the imbalance of host T cells. Galectin-9 negatively regulates CD4 effector cell (Th1 and Th17) function by binding to Tim-3. However, relationship between Galectin-9/Tim-3 CD4+ subsets in patients with aGVHD after Haplo-HSCT (haploidentical peripheral blood hematopoietic stem transplantation) has not been fully elucidated. Here, we investigated role haplo-HSCT. Forty-two underwent (26 without 16 aGVHD), 20 healthy controls were included....

The present study investigated the immunopathological effect of Echinococcus multilocularis (Em) using cytokine detection. Expression T‑helper (Th) 17‑cytokine, interleukin 17 (IL‑17), was observed immunohistochemical staining, and levels cytokines, including IL‑17, transforming growth factor β1 (TGF‑β1), IL‑6, interferon γ (IFN‑γ) IL‑4, were assessed ELISA at different stages infection. IL‑17 expression occurred in hepatic cells 1 month post‑infection, reached a maximum 3 months...

The interaction between Tim-3 on T cell and its ligand, Galectin-9, negatively regulates cellular immune responses. However, the role of Tim-3/Galectin-9 pathway in evasion cervical cancer remains unknown. This study is to investigate expression, function, regulation signaling human papilloma virus (HPV) positive cancer. Flow cytometry showed that expression Galectin-9 monocytes HPV patients were significantly higher compared intraepithelial neoplasia benign uterine fibroids + CD4+ Th1 cells...

Cystic echinococcosis (CE) is a global zoonosis caused by the larvae of parasite Echinococcus granulosus (E. granulosus). According to its life cycle and previous studies antigen candidates for vaccines against E. granulosus, we chose two proteins expressed at different stages design multi-epitope vaccine. The EgA31 gene derived from adult stage EgG1Y162 larval granulosus. In this study, used several bioinformatics methods analyze various aspects proteins, including physicochemical...

Peri-implant disease (PID) is a general term for inflammatory diseases of soft and hard tissues that occur around implants, including peri-implant mucositis peri-implantitis. Cytokines are class small molecule proteins, which have various functions such as regulating innate immunity, adaptive repairing damaged tissues. In order to explore the characteristics clinical significance tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, growth (TGF)-β1 expression levels in serum patients...

In our study, we investigated whether circulating T follicular helper (Tfh) and the related cytokines are involved in human cystic echinococcosis (CE). A total of 64 patients with CE 30 healthy controls were enrolled this study. Percentages CCR7loPD-1hi cells within CXCR5+ CD4+ (circulating Tfh cells) detected by flow cytometry. Levels IL-21 IL-4 peripheral blood cytometric bead array. The mRNA expression IL-21, IL-4, Bcl-6, Blimp-1 mononuclear (PBMCs) measured real-time PCR. IgG1, IgG2,...

Asthma is a chronic lung inflammation which affects many people. As current therapies for asthma mainly rely on administration of glucocorticoids and have side effects, new therapy needed. In this study, we investigated Nepeta bracteata Benth., traditional Uygur Herb, its therapeutics effect in OVA induced asthmatic mice model. Treatment sensitized with extract from Benth. demonstrated improved pathology, as well reduced infiltration eosinophil neutrophil. also contributed to the rebalance...

Abstract Hepatocellular carcinoma (HCC) circulating tumor cells (CTCs) exhibit significant phenotypic heterogeneity and diverse gene expression profiles due to epithelial‐mesenchymal transition (EMT). However, current detection methods lack the capacity for simultaneous quantification of multidimensional biomarkers, impeding a comprehensive understanding biology dynamic changes. Here, CTC Digital Simultaneous Cross‐dimensional Output Unified Tracking (d‐SCOUT) technology is introduced, which...

Galectin-9 and myeloid-derived suppressor cells (MDSCs) have an important role in tumors, but their clinical values chronic lymphocytic leukemia (CLL) not been fully elucidated. This study aimed to analyze the prognosis of MDSCs CLL. The concentrations Galectin-9, argininase-1, inducible nitric oxide synthase serum were detected by enzyme-linked immune sorbent assay. expression Tim-3 protein peripheral blood mononuclear cell was Western blot. Flow cytometry used percentages on T-cells (CD3+...

Excessive activation of M1 macrophages affects the chronic inflammatory response airways and leads to development obstructive pulmonary disease (COPD). Therefore, it needs be closely monitored investigated. MAPK signaling pathway is involved in macrophages, N6-methyladenosine (m6A) pathogenesis COPD. However, unknown whether mediated by m6A COPD.The GEO data were analyzed using bioinformatics techniques assess differences between COPD healthy individuals levels their secreted cytokines,...

The present study is to measure the expression of programmed death (PD)-1 / ligand-1 (PD-L1) negative costimulatory molecules, soluble format sPD-1 in patients with immune thrombocytopenia (ITP), and investigate their correlation secretion cytokines. A total 35 ITP were included study. Twenty healthy subjects who received physical examination at our hospital as control group. Peripheral blood was collected from all subjects. Flow cytometry performed determine percentages PD-1+CD4+T cells...

The influence of mild perioperative hypothermia on the immune function and incidence postoperative wound infections has been suggested, but specific mechanism is unclear. This study aimed to analyze body temperature, function, infection rates in patients receiving open surgery for gastric cancer. Body temperature was controlled each patient using one four different methods: wrapping limbs, head neck; insulated blankets; warming infusion fluids without blankets. One hundred were randomly...