Emerging evidence demonstrates that insufficient migration and invasion of trophoblasts play critical roles in the pathogenesis recurrent spontaneous abortion (RSA). Cell-to-cell communication at maternal-fetal interface is essential to maintain trophoblasts. M1 macrophages, important immune cellular components interface, have been reported be elevated decidua tissues from patients with RSA. Recent studies indicate macrophages modulate trophoblast biological behaviors; however, underlying...

Abstract Trophoblasts are important parts of the placenta and exert vital roles in maternal‐foetal crosstalk, sufficient trophoblasts migration invasion is critical for embryo implantation normal pregnancy. Macrophages, as major components decidual microenvironment at interface, can interact with to participate regulation Previously, our group have demonstrated that could induce macrophages polarization M2 subtype by secreting interleukin‐6 (IL‐6); however, understanding regulating limited....

Insufficient invasion ability of trophoblasts might be associated with the development recurrent miscarriage (RM). Ubiquitin-specific protease 25 (USP25) can regulate processes and migration in different types cancer cells. However, effect USP25 on its roles RM are unknown. In this study, we first analyzed expression placental villous tissues from patients, then assessed epithelial-to-mesenchymal transition (EMT), trophoblasts. Furthermore, bioinformatics prediction luciferase reporter assay...

Abstract Background The fine balance of Th17/Treg is crucial for maintenance immune homeostasis. objective this study was to investigate the and expression related cytokines in Uighur cervical cancer patients. Methods Peripheral blood collected from 65 cases patients, 42 CIN patients 40 healthy people. Flow cytometry used detect percentages T cell subsets, including CD3 + cells, CD4 CD8 Treg cells Th17 cells. ELISA assay conducted levels TGF-β, IL-6, IL-10, IL-17, IL-23 IFN-γ. Results There...

Although methotrexate (MTX) is commonly used for the treatment of choriocarcinoma, chemoresistance to MTX may occur in a considerable fraction patients. Further understanding on mechanisms resistance would help develop more effective therapy choriocarcinoma.Quantitative proteomic approach involving TMT labeling and LC-MS/MS was identify resistance-related profiles choriocarcinoma cell models. Pathway process enrichment analysis were conducted biological processes/molecular pathways. CCK-8...

It is known that chronic HBV infection (CHB) the major risk factor for hepatocellular carcinoma (HCC) because CHB could not only cause liver tumorigenesis but also lead to change of local microenviroment and lower immune response infected cancerous cells (immune tolerance). Human leucocyte antigen-G (HLA-G) belongs a non-classic MHC-I family was considered be an tolerance molecule, which bind immunosuppressive receptors natural killer cell (NK) T trigger signaling. Recently, numerous studies...

Chemotherapy resistance is the major issue of choriocarcinoma. Apoptosis always ultimate outcome chemotherapeutic drugs, which considered one reasons resistance. We investigated role STAT3/NFIL3 signaling-inhibited apoptosis in chemotherapy and whether Raddeanin A (RA) could be a new drug to reverse Established three drug-resistant cell lines as JEG-3/MTX, JEG-3/5-FU, JEG-3/VP16. NFIL3 STAT3 expression was evaluated cells. The IC50 value, rate apoptins were observed with transfection...

Recurrent pregnancy loss (RPL) refers to two or more spontaneous abortions that occur consecutively with the same spouse. RPL severely affects human reproduction health, and causes extreme physical mental suffering patients their families.We used isobaric tags for relative absolute quantitation (iTRAQ), which was coupled liquid chromatography mass spectrometry (LC-MS) proteomic analysis, in order identify differentially expressed proteins. Moreover, we western blot analyze proteins.Of 2350...

Inhibitor of DNA ‐binding protein 3 (Id3) is required for tumor angiogenesis and regulatory T‐cell generation. However, the involvement Id3 in unexplained repeated implantation failure ( RIF ) recurrent miscarriage RM remains poorly understood. Immunohistochemistry was used to identify Id3, CD 34, CTLA ‐4, FOXP endometrium taken from women with (n=16), (n=16) matched controls (n=8). The images were acquired analyzed by Vectra ® automated quantitative pathology imaging system. Percentage +...

Dexamethasone (DEX) is a well-known anti-inflammatory drug, whose widespread clinical use nevertheless restricted by its serious side effects. By conjugation of DEX with C60, we found that this nanomedicine retained the activity while reducing effects in animal model. In mouse thymocytes, CCK-8 assay showed cytotoxicity DEX-C60 was significantly lower than free DEX. Flow cytometric studies revealed incubation induced much less apoptotic thymocytes. Interestingly, such reduced and apoptosis...

Senescence is involved in many complications of pregnancy. However, whether senescent changes are also associated with missed miscarriage has not been fully investigated.The levels p16, p21, and γH2AX, markers senescence, were measured placentas collected from women by immunohistochemistry Western blotting. Levels misfolded proteins or normal first-trimester placenta that had treated H2O2 (100 μM) extracellular vesicles (EVs) placental explant culture fluorescent compound, thioflavin-T. The...

Toll-like receptor 4 (TLR4), a recently identified vertebrate receptor, serves pivotal role in immune responses. The aim of the present study was to investigate association between human TLR4 gene and recurrent spontaneous miscarriage (RSM). A total 306 RSM patents age-matched controls were genotyped for four single-nucleotide polymorphisms (SNPs) (rs1927914, rs1927911, rs4986790 rs4986791). Data analyzed Uygur Han women separately using haplotype-based case-control study. There significant...

Human leukocyte antigen (HLA)-G exhibits immunotolerogenicity and is related to allograft acceptance. Qa-2 the murine homolog of HLA-G; it has structure functions similar those HLA-G. We investigated dynamic expression in skin allografts by immunohistochemistry on peripheral blood lymphocyte subsets flow cytometry during entire process acute graft rejection (AGR) with a transplantation model determine its relationship pathological changes influence immunosuppressive therapy. In grafts...

Liver metabolites are involved in the progression of rheumatoid arthritis (RA), indicating a connection between liver and joints. However, impact mechanism Hepatitis B virus (HBV), hepatotropic virus, on RA still unclear. We investigated correlation HBV using Mendelian randomization analysis. Single-cell transcriptome analysis was conducted to investigate changes cell subtypes synovial tissue HBV-RA patients. Fibroblast-like synoviocytes (FLS) were used create model, examined identify key...

Objective To investigate the regulatory effect of CsA on expression NK cell inhibitory receptor ILT4 and cytotoxicity cells.Methods cells treated with ( 10 mg/L) or DMSO for 12,24 36 h were chosen as three experimental groups control respectively.RTqPCR flow cytometry performed to detect alteration at mRNA protein level respectively.The HLA-G in human gastric cancer line BGC-823 placental choriocarcinoma JEG-3 measured same time,and then cytolytic activity untreated against was determined...