A5019514245- Bone and Dental Protein Studies
- Cell Adhesion Molecules Research
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Pancreatic and Hepatic Oncology Research
- Blood properties and coagulation
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Animal Genetics and Reproduction
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
Aarhus University
2014-2017
Aarhus University Hospital
2017
Transgenic porcine cancer models bring novel possibilities for research. Their physical similarities with humans enable the use of surgical procedures and treatment approaches used patients, which facilitates clinical translation. Here, we aimed to develop an inducible oncopig model intestinal cancer. (TG) minipigs were generated using somatic cell nuclear transfer by handmade cloning. The pigs encode two TG cassettes: (a) Flp recombinase-inducible oncogene cassette containing KRAS-G12D,...
Osteopontin (OPN) is a highly posttranslationally modified protein present in several tissues where it implicated numerous physiological processes. OPN primarily exerts its functions through interaction with integrins via the Arg-Gly-Asp and Ser-Val-Val-Tyr-Gly-Leu-Arg sequences located N-terminal part of protein. can be polymerized by cross-linking enzyme transglutaminase 2 (TG2), polymerization has been shown to enhance biological activity OPN. However, little known about reactivity...
Recombinase mediated cassette exchange (RMCE) is a powerful tool for targeted insertion of transgenes. Here we describe non-proprietary 'RMCE-in' cell lines as an alternative to the 'Flp-in' system and lines. RMCE-in offer number advantages including increased efficiency integration genetic element interest (GEI) at single docking site, lack bacterial backbone site both before after GEI integration, removal selection visual markers initially present upon possibility validate by loss red...