Weiwei Zhai
A5039441451- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- Arsenic contamination and mitigation
- RNA modifications and cancer
- Evolution and Genetic Dynamics
- Chromium effects and bioremediation
- Heavy metals in environment
- Ferroptosis and cancer prognosis
- Immune cells in cancer
- Bat Biology and Ecology Studies
- Genetic diversity and population structure
- Physiological and biochemical adaptations
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Viral Infections and Vectors
- Cytokine Signaling Pathways and Interactions
- HER2/EGFR in Cancer Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
Chinese Academy of Sciences
2014-2025
Institute of Zoology
2019-2025
Soochow University
2016-2025
First Affiliated Hospital of Soochow University
2016-2025
University of Chinese Academy of Sciences
2022-2025
Aerospace Information Research Institute
2025
Agency for Science, Technology and Research
2015-2024
Genome Institute of Singapore
2015-2024
Zhejiang University of Technology
2021-2024
DHC Software (China)
2024
Neandertals, the closest evolutionary relatives of present-day humans, lived in large parts Europe and western Asia before disappearing 30,000 years ago. We present a draft sequence Neandertal genome composed more than 4 billion nucleotides from three individuals. Comparisons to genomes five humans different world identify number genomic regions that may have been affected by positive selection ancestral modern including genes involved metabolism cognitive skeletal development. show...
Whole-genome data indicate that early modern humans expanded into Australia 62,000 to 75,000 years ago.
The origin and evolution of the domestic dog remains a controversial question for scientific community, with basic aspects such as place date origin, number times dogs were domesticated, open to dispute. Using whole genome sequences from total 58 canids (12 gray wolves, 27 primitive Asia Africa, collection 19 diverse breeds across world), we find that southern East have significantly higher genetic diversity compared other populations, are most basal group relating indicating an ancient in...
As the current therapeutic strategies for human hepatocellular carcinoma (HCC) have been proven to limited effectiveness, immunotherapy becomes a compelling way tackle disease. We aim provide humanised mouse (humice) models understanding of interaction between cancer and immune system, particularly human-specific drug testing.
Hypoxia is one of the central players in shaping immune context tumor microenvironment (TME). However, complex interplay between cell infiltrates within hypoxic TME HCC remains to be elucidated.We analyzed landscapes hypoxia-low and hypoxia-high regions using cytometry by time light, immunohistochemistry, transcriptomic analyses. The mechanisms immunosuppression subsets interest were further explored vitro hypoxia assays. Regulatory T cells (Tregs) a number immunosuppressive myeloid subsets,...
The clinical relevance of immune landscape intratumoural heterogeneity (immune-ITH) and its role in tumour evolution remain largely unexplored. Here, we uncover significant spatial phenotypic immune-ITH from multiple sectors decipher relationship with disease progression hepatocellular carcinomas (HCC). Immune-ITH is associated transcriptomic-ITH, mutational burden distinct microenvironments. Tumours low experience higher immunoselective pressure escape via loss heterozygosity human...
Spatially resolved transcriptomics involves a set of emerging technologies that enable the transcriptomic profiling tissues with physical location expressions. Although variety methods have been developed for data integration, most them are single-cell RNA-seq datasets without consideration spatial information. Thus, can integrate from multiple tissue slides, possibly individuals, needed. Here, we present PRECAST, integration method complex batch effects and/or biological between slides....
While immune checkpoint blockade (ICB) has shown promise in patients with hepatocellular carcinoma (HCC), it is associated modest response rates and immune-related adverse events (irAEs) are common. In this study, we aimed to decipher trajectories mechanisms of and/or irAEs HCC receiving anti-programmed cell death 1 (anti-PD-1) therapy.Pre- on-treatment peripheral blood samples (n = 60) obtained from 32 (Singapore cohort) were analysed by cytometry time-of-flight single-cell RNA sequencing,...
Genealogical patterns in different genomic regions may be due to the joint influence of gene flow and selection. The existence two subspecies cultivated rice provides a unique opportunity for analyzing these effects during domestication. We chose 66 accessions from three taxa (about 22 each Oryza sativa indica, O. japonica, rufipogon) whole-genome sequencing. In search signature selection, we focus on low diversity (LDRs) shared by both cultivars. found that genealogical histories...
Abstract EGFR -mutant lung adenocarcinomas (LUAD) display diverse clinical trajectories and are characterized by rapid but short-lived responses to tyrosine kinase inhibitors (TKIs). Through sequencing of 79 spatially distinct regions from 16 early stage tumors, we show that despite low mutation burdens, Asian LUADs unexpectedly exhibit a complex genomic landscape with frequent whole-genome doubling, aneuploidy, high clonal diversity. Multiple truncal alterations, including TP53 mutations...
Abstract Hepatocellular carcinoma (HCC) has one of the poorest survival rates among cancers. Using multi-regional sampling nine resected HCC with different aetiologies, here we construct phylogenetic relationships these sectors, showing diverse levels genetic sharing, spanning early to late diversification. Unlike variegated pattern found in colorectal cancers, a large proportion display clear isolation-by-distance where spatially closer sectors are genetically more similar. Two...
We present the analysis of evolution tumors in a case hepatocellular carcinoma. This is particularly informative about cancer growth dynamics and underlying driving mutations. sampled nine different sections from three seven more adjacent nontumor tissues. Selected were subjected to exon as well whole-genome sequencing. Putative somatic mutations then individually validated across all 9 tumor 7 sections. Among validated, 24 amino acid changes; addition, 22 large indels/copy number variants...
The high-altitude hypoxic environment represents one of the most extreme challenges for mammals. Previous studies humans on Tibetan plateau and in Andes Mountains have identified statistical signatures selection different sets loci. Here, we first measured hemoglobin levels village dogs from Tibet those Chinese lowlands. We found that are very similar between two groups, suggesting might share adaptive strategies as people. Through a whole-genome sequencing approach, EPAS1 HBB candidate...
Mesenchymal epithelial transition factor ( MET) activation has been implicated as an oncogenic driver in epidermal growth receptor EGFR)-mutant non-small-cell lung cancer (NSCLC) and can mediate primary secondary resistance to EGFR tyrosine kinase inhibitors (TKI). High copy number thresholds have suggested enrich for response MET inhibitors. We examined the clinical relevance of gain (CNG) setting treatment-naive metastatic EGFR-mutant-positive NSCLC.
ABSTRACT Dogs are the most phenotypically diverse mammalian species, and they possess more known heritable disorders than any other non-human mammal. Efforts to catalog characterize genetic variation across well-chosen populations of canines necessary advance our understanding their evolutionary history architecture. To date, no organized effort has been undertaken sequence world's canid populations. The Dog10K Consortium (http://www.dog10kgenomes.org) is an international collaboration...
Bioavailability and speciation of arsenic (As) are impacted by fertilization bacteria in the rice rhizosphere. In this study, we investigated effects long-term manure application on As bioavailability, microbial community structure, functional genes a paddy field. The results showed that did not affect total soil but increased soluble forms 19%, increasing arsenite (As(III)) accumulation grains roots 34 64% compared to control. A real-time quantitative polymerase chain reaction (qPCR)...