A5008293185- Forensic and Genetic Research
- Forensic Anthropology and Bioarchaeology Studies
- Pleistocene-Era Hominins and Archaeology
- Archaeology and ancient environmental studies
- Genomics and Phylogenetic Studies
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- Genetic diversity and population structure
- Yersinia bacterium, plague, ectoparasites research
- Evolution and Paleontology Studies
- Wildlife Ecology and Conservation
- Pacific and Southeast Asian Studies
- SARS-CoV-2 detection and testing
- Marine and environmental studies
- Race, Genetics, and Society
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Primate Behavior and Ecology
- Nuclear Physics and Applications
- Paleopathology and ancient diseases
- Bat Biology and Ecology Studies
- Marine animal studies overview
- Zoonotic diseases and public health
Max Planck Institute for Evolutionary Anthropology
2015-2024
Max Planck Society
2012-2024
Max Planck Institute for Evolutionary Biology
2024
Bundeswehr Medical Service Academy
2024
Romanian Academy
2023
University of Bucharest
2023
Pennsylvania State University
2023
Institute of Vertebrate Paleontology and Paleoanthropology
2017
Chinese Academy of Sciences
2017
Université Claude Bernard Lyon 1
2005
Neandertals, the closest evolutionary relatives of present-day humans, lived in large parts Europe and western Asia before disappearing 30,000 years ago. We present a draft sequence Neandertal genome composed more than 4 billion nucleotides from three individuals. Comparisons to genomes five humans different world identify number genomic regions that may have been affected by positive selection ancestral modern including genes involved metabolism cognitive skeletal development. show...
INTRODUCTION The large amount of DNA sequence data generated by high-throughput sequencing technologies often allows multiple samples to be sequenced in parallel on a single run. This is particularly true if subsets the genome are studied rather than complete genomes. In recent years, target capture from libraries has largely replaced polymerase chain reaction (PCR) as preferred method enrichment. Parallelizing and for requires incorporation sample-specific barcodes into libraries, which...
We present a DNA library preparation method that has allowed us to reconstruct high-coverage (30×) genome sequence of Denisovan, an extinct relative Neandertals. The quality this allows direct estimation Denisovan heterozygosity indicating genetic diversity in these archaic hominins was extremely low. It also tentative dating the specimen on basis "missing evolution" its genome, detailed measurements and Neandertal admixture into present-day human populations, generation near-complete...
Using DNA extracted from a finger bone found in Denisova Cave southern Siberia, we have sequenced the genome of an archaic hominin to about 1.9-fold coverage. This individual is group that shares common origin with Neanderthals. population was not involved putative gene flow Neanderthals into Eurasians; however, data suggest it contributed 4–6% its genetic material genomes present-day Melanesians. We designate this 'Denisovans' and may been widespread Asia during Late Pleistocene epoch. A...
Significance Outside of permafrost, no contiguous DNA sequences have been generated from material older than ∼120,000 y. By improving our ability to sequence very short fragments, we recovered the mitochondrial genome a >300,000-y-old cave bear Sima de los Huesos, Spanish site that is famous for its rich collection Middle Pleistocene human fossils. This finding demonstrates can survive hundreds thousands years outside permafrost and opens prospect making more samples this time period...
Due to the increasing throughput of current DNA sequencing instruments, sample multiplexing is necessary for making economical use available capacities. A widely used strategy Illumina Genome Analyzer utilizes sample-specific indexes, which are embedded in one library adapters. However, this and similar multiplex approaches come with a risk misidentification. By introducing indexes into both adapters (double indexing), we have developed method that reveals rate misidentification within...
Many waterbodies across the United States do not meet water quality standards. To help determine where and to what extent improvements should be sought, policymakers must consider costs of regulations with their monetized values. We ...Scientific knowledge related quantifying benefits for landscape-wide does current regulatory benefit–cost analysis needs in States. In this study we addressed gap by ...
The latest DNA recovery and sequencing technologies have been used to reconstruct the genome of Yersinia pestis bacterium responsible for Black Death pandemic bubonic plague that spread across Europe in fourteenth century. was pieced together from total extracted skeletal remains four individuals excavated a large cemetery on site Royal Mint East Smithfield London, where more than 2,000 victims were buried 1348 1349. draft sequence does not differ substantially modern Y. strains, providing...
To date, the only Neandertal genome that has been sequenced to high quality is from an individual found in Southern Siberia. We of a female ~50,000 years ago Vindija Cave, Croatia, ~30-fold genomic coverage. She carried 1.6 differences per 10,000 base pairs between two copies her genome, fewer than present-day humans, suggesting populations were small size. Our analyses indicate she was more closely related Neandertals mixed with ancestors humans living outside sub-Saharan Africa previously...
Hominins with morphology similar to present-day humans appear in the fossil record across Eurasia between 40,000 and 50,000 y ago. The genetic relationships these early modern human populations have not been established. We extracted DNA from a 40,000-y-old anatomically Tianyuan Cave outside Beijing, China. Using highly scalable hybridization enrichment strategy, we determined sequences of mitochondrial genome, entire nonrepetitive portion chromosome 21 (∼30 Mbp), over 3,000 polymorphic...
DNA sequences determined from ancient organisms have high error rates, primarily due to uracil bases created by cytosine deamination. We use synthetic oligonucleotides, as well extracted mammoth and Neandertal remains, show that treatment with uracil-DNA-glycosylase endonuclease VIII removes residues repairs most of the resulting abasic sites, leaving undamaged parts fragments intact. this protocol greatly increased accuracy. In addition, our results demonstrate retains in vivo patterns CpG...
Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity hominin fossils often impedes understanding which hominins occupied site. Using targeted enrichment mitochondrial DNA, we show that cave sediments represent source ancient mammalian DNA includes traces even at sites and in layers where no remains have been discovered. By automation-assisted screening numerous sediment samples, detected Neandertal eight from four caves Eurasia. In Denisova...