Khi Pin Chua
A5000859894- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Cytokine Signaling Pathways and Interactions
- Colorectal Cancer Treatments and Studies
- Ferroptosis and cancer prognosis
- Bioinformatics and Genomic Networks
- RNA modifications and cancer
- Williams Syndrome Research
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Congenital Heart Disease Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastric Cancer Management and Outcomes
- Genomics and Phylogenetic Studies
- Genomics and Rare Diseases
- Congenital heart defects research
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Lung Cancer Diagnosis and Treatment
- Advanced Breast Cancer Therapies
- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Genetic Neurodegenerative Diseases
- Crystallography and molecular interactions
Genome Institute of Singapore
2018-2024
Pacific Biosciences (United States)
2021-2024
Agency for Science, Technology and Research
2020-2024
Nanyang Technological University
2016-2018
Intra-tumor heterogeneity (ITH) is a key challenge in cancer treatment, but previous studies have focused mainly on the genomic alterations without exploring phenotypic (transcriptomic and immune) heterogeneity. Using one of largest prospective surgical cohorts for hepatocellular carcinoma (HCC) with multi-region sampling, we sequenced whole genomes paired transcriptomes from 67 HCC patients (331 samples). We found that while ITH was rather constant across stages, had very different...
Highlights•Profilin isoforms have extended N terminus in addition to the conserved folded region•Intrinsically disordered region regulates oligomerization of AtPRF3•AtPRF3 inhibits formin-mediated actin nucleation•PAMP-triggered immunity and AtPRF3 turnover regulate each other a feedback loopSummaryProfilin functions with formin assembly, process that multiple aspects plant development immune responses. High-level eukaryotes contain profilin, formin, actin, whose partner-specific...
Abstract Purpose: Despite the established role of EGFR tyrosine kinase inhibitors (TKIs) in EGFR-mutated NSCLC, drug resistance inevitably ensues, with a paucity treatment options especially EGFRT790M-negative resistance. Experimental Design: We performed whole-exome and transcriptome analysis 59 patients first- second-generation TKI-resistant metastatic NSCLC to characterize compare molecular alterations mediating T790M-positive (T790M+) -negative (T790M−) disease. Results: Transcriptomic...
Hepatocellular carcinoma (HCC) is a highly fatal cancer characterized by high intra-tumor heterogeneity (ITH). A panoramic understanding of its tumor evolution, in relation to clinical trajectory, may provide novel prognostic and treatment strategies.
Age-related gut microbial changes have been widely investigated over the past decade. Most of previous age-related microbiome studies were conducted on Western population, and short-read sequencing (e.g., 16S V4 or V3-V4 region) was most common microbiota profiling method. We evaluated compositional differences using long-read approach (i.e., PacBio targeting full-length V1-V9 regions) to enable a deeper taxonomic resolution better characterize Singaporeans from different age groups.
Aggregation of proteins into amyloid is the central hallmark a number protein diseases. Most studies were carried out on aggregation between similar species. However, it was observed that some patients with certain disease can easily acquire another unrelated disease. As such, also important to examine different Usually same species be attributed similarity their respective amino acid sequences. In this article, we motivated by an experimental study amylin (Islet Amyloid Polypeptide, IAPP)...
Neurodevelopmental disorders (NDDs) comprise conditions that emerge during the child's development and contribute significantly to global health economic burdens. De novo variants in CNOT3 have been linked NDDs understanding genotype-phenotype relationship between will aid improving diagnosis management.
Recent advances in genome sequencing have improved variant calling complex regions of the human genome. However, it is difficult to quantify performance since existing standards often focus on specificity, neglecting completeness analyze regions. To create a more comprehensive truth set, we used Mendelian inheritance large pedigree (CEPH-1463) filter variants across Illumina, PacBio high-fidelity (HiFi), and Oxford Nanopore Technologies platforms. This generated map with over 4.7 million...
Abstract Tumor evolution is a highly heterogeneous process where multiple oncogenic pathways can lead to host defense evasion. Advancements in genome sequencing are allowing us better understand these processes and their impact. However, critical genomic variations that could be pivotal the development of tumors may have remained undetected due technological constraints short-read sequencing. PacBio HiFi generates accurate (>99.9%) long reads (>15 kb) with native...
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
Supplementary Data from Integrative Profiling of T790M-Negative EGFR-Mutated NSCLC Reveals Pervasive Lineage Transition and Therapeutic Opportunities
<div>AbstractPurpose:<p>Despite the established role of EGFR tyrosine kinase inhibitors (TKIs) in <i>EGFR</i>-mutated NSCLC, drug resistance inevitably ensues, with a paucity treatment options especially <i>EGFR</i><sup>T790M</sup>-negative resistance.</p>Experimental Design:<p>We performed whole-exome and transcriptome analysis 59 patients first- second-generation TKI-resistant metastatic NSCLC to characterize compare molecular...
<div>AbstractPurpose:<p>Despite the established role of EGFR tyrosine kinase inhibitors (TKIs) in <i>EGFR</i>-mutated NSCLC, drug resistance inevitably ensues, with a paucity treatment options especially <i>EGFR</i><sup>T790M</sup>-negative resistance.</p>Experimental Design:<p>We performed whole-exome and transcriptome analysis 59 patients first- second-generation TKI-resistant metastatic NSCLC to characterize compare molecular...