A5017352968- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Adipose Tissue and Metabolism
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- Ferroptosis and cancer prognosis
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Colorectal Cancer Treatments and Studies
St. Jude Children's Research Hospital
2021
National Cancer Centre Singapore
2016-2021
A population of cell clusters circulating in cancer patients consist tumor-derived endothelial cells reflecting features the tumor vasculature.
Abstract Profiling of circulating tumor DNA (ctDNA) may offer a non-invasive approach to monitor disease progression. Here, we develop quantitative method, exploiting local tissue-specific cell-free (cfDNA) degradation patterns, that accurately estimates ctDNA burden independent genomic aberrations. Nucleosome-dependent cfDNA at promoters and first exon-intron junctions is strongly associated with differential transcriptional activity in tumors blood. A model, based on just 6 regulatory...
e15531 Background: Response to immune checkpoint inhibition is dismal in colorectal cancer (CRC). There are limited experimental models evaluate immunotherapy human CRC. We developed an ex vivo co-culture system interrogate pharmacologic or genetic perturbations that modulate anti-cancer immunotoxicity Methods: Our uses expanded CD8+ TILs & peripheral blood mononuclear cells (PBMC), cultured up 6 weeks with engineered auxotrophic K562 aPCs, as effector cells. For target cells, we use both...
Abstract Background: Immunotherapy has so far had limited success in colorectal cancer (CRC), with its efficacy restricted to a subset of microsatellite instability high (MSI-H) tumors. A comprehensive interrogation the CRC tumor immune microenvironment (TME) is urgently needed. We present here an ongoing multi-platform study on early stage colon and rectal cancers, where immuno-genomic profiling tumors patient-derived cell models epithelia, cancer-associated fibroblasts tumor-infiltrating...
In the context of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC), despite a range possibilities, antigen specificities tumor-infiltrating T cells and their relevance to control is largely unknown. Using highly multiplexed peptide-MHC tetramer staining unexpanded from blood, liver tumor tissues 46 HCC patients, we detected 91 different CD8 cell populations specific for epitopes derived HBV, tumor-associated neoantigens (NeoAg), as well disease-unrelated antigens. Parallel...