A5064361576- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Renal and related cancers
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Chemokine receptors and signaling
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cell Adhesion Molecules Research
- Cancer therapeutics and mechanisms
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
Japan Science and Technology Agency
2024
Agency for Science, Technology and Research
2018-2024
Genome Institute of Singapore
2018-2020
Chemo-resistance is one of the major causes cancer-related deaths. Here we used single-cell transcriptomics to investigate divergent modes chemo-resistance in tumor cells. We observed that higher degree phenotypic intra-tumor heterogeneity (ITH) favors selection pre-existing drug-resistant cells, whereas phenotypically homogeneous cells engage covert epigenetic mechanisms trans-differentiate under drug-selection. This adaptation was driven by selection-induced gain H3K27ac marks on...
<title>Abstract</title> Mechanisms for resistance to cytotoxic cancer drugs are dependent on dynamic changes in the biochemistry of cellular pathways, information which is hard obtain at systems level. Here we use a deep functional proteomics implementation CETSA (Cellular Thermal Shift Assay) revealing range induced biochemical responses gemcitabine resistant and sensitive diffuse large B cell lymphoma (DLBCL) lines. Initial both, cells, reflect known targeted effects by ribonucleotide...
e15531 Background: Response to immune checkpoint inhibition is dismal in colorectal cancer (CRC). There are limited experimental models evaluate immunotherapy human CRC. We developed an ex vivo co-culture system interrogate pharmacologic or genetic perturbations that modulate anti-cancer immunotoxicity Methods: Our uses expanded CD8+ TILs & peripheral blood mononuclear cells (PBMC), cultured up 6 weeks with engineered auxotrophic K562 aPCs, as effector cells. For target cells, we use both...
86 Background: Natural killer (NK) cells play promising roles in cancer surveillance and possess multiple unique characteristics for allogenic cell transfer (ACT) the immunotherapy. However, most studies were applicable to ex vivo expanded NK from peripheral blood, which requires massive amount of numbers initial isolation expansion. Moreover, recent have demonstrated a phenotypic heterogeneity between tissue resident cells. Methods: In this study, we aim establish expansion tumor...