A5058328532- Advanced Proteomics Techniques and Applications
- Click Chemistry and Applications
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Educational Reforms and Innovations
- Metabolomics and Mass Spectrometry Studies
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
- Chinese history and philosophy
- Protein purification and stability
- Medical Research and Treatments
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Microbial Natural Products and Biosynthesis
- 2D Materials and Applications
- Global Educational Reforms and Inequalities
- MXene and MAX Phase Materials
- Peptidase Inhibition and Analysis
- Regional Development and Environment
- Analytical Chemistry and Chromatography
- Cancer therapeutics and mechanisms
- Streptococcal Infections and Treatments
- Higher Education Governance and Development
Karolinska Institutet
2024-2025
Karolinska University Hospital
2025
Dalian Institute of Chemical Physics
2018-2024
Chinese Academy of Sciences
2018-2024
University of Chinese Academy of Sciences
2018-2024
First Affiliated Hospital of Henan University
2024
Shanghai Jinyuan Senior High School
2024
Qingdao Agricultural University
2022
Existing thermal shift-based mass spectrometry approaches are able to identify target proteins without chemical modification of the ligand, but they suffering from complicated workflows with limited throughput. Herein, we present a new method, termed matrix shift assay (mTSA), for fast deconvolution ligand-binding targets and binding affinities at proteome level. In mTSA, sample matrix, treated horizontally five different compound concentrations vertically technical replicates each...
The KIT/c-KIT proto-oncogene is frequently over-expressed in Merkel cell carcinoma (MCC), an aggressive skin cancer commonly caused by polyomavirus (MCPyV). Here, we demonstrated that truncated MCPyV-encoded large T-antigen (LT) suppressed macroautophagy/autophagy stabilizing and sequestering KIT the paranuclear compartment via binding VPS39. engaged with phosphorylated BECN1, thereby enhancing its association BCL2 while diminishing interaction PIK3C3 complex. This process ultimately...
For the first time, MAX-Ti<sub>3</sub>AlC<sub>2</sub> and 2D MXene-Ti<sub>3</sub>C<sub>2</sub> were used as affinity chromatography stationary phases for specific enrichment of phosphopeptides, they provided complementary coverage phosphoproteomes.
Cell surface is the primary site for sensing extracellular stimuli. The knowledge of transient changes on surfaceome upon a perturbation very important as initial changed proteins could be driving molecules some phenotype. In this study, we report fast cell labeling strategy based peroxidase-mediated oxidative tyrosine coupling strategy, enabling efficient and selective within seconds. With time 1 min, 2684 proteins, including 1370 (51%) surface-annotated (cell surface/plasma...
In this study, we presented an enrichment-free approach for the sensitive analysis of protein phosphorylation in minute amounts samples, such as purified complexes. This method takes advantage high sensitivity parallel reaction monitoring (PRM). Specifically, low confident phosphopeptides identified from data-dependent acquisition (DDA) data set were used to build a pseudotargeted list PRM allow identification additional with confidence. The development targeted is very easy same sample and...
While thermal proteome profiling (TPP) shines in the field of drug target screening by analyzing soluble fraction samples treated at high temperature, counterpart, insoluble precipitate, has been overlooked for a long time. The analysis precipitate is hampered inefficient sample processing procedure. Herein, we propose novel method, termed microparticle-assisted precipitation (MAPS), identification. MAPS method exploits principle that drug-bound proteins will be more resistant to unfolding...
ABSTRACT The potential of CRISPR/Cas systems for nucleic acid detection in novel biosensing applications is remarkable. current clinical diagnostic Streptococcus pyogenes ( S. ) based on serological identification, culture, and PCR. We report a rapid, simple, sensitive method detecting screening . This promising supplemental test. After 10 min the sample processing recombinase polymerase amplification, followed by Cas12 reaction 3 lateral flow biosensor (LFB) readout, visible outcome can be...
Target proteins are often stabilized after binding with a ligand and thereby typically become more resistant to denaturation. Based on this phenomenon, several methods without the need covalently modify have been developed identify target for specific ligand. These usually employ complicated workflows high cost limited throughput. Here, we develop an iso-pH shift assay (ipHSA) method, proteome-wide identification method that detects ligand-induced protein solubility shifts by precipitating...
Although capillary liquid chromatography married with tandem mass spectrometry (cLC–MS/MS) has become a powerful technique for proteomics and metabolomics research, it is still great challenge to fabricate durable capillary-based analytical columns coupling continuous nanoflow (<1 000 nL/min) electrospray ionization (ESI) MS, owing the issue of clogging fragile emitters. Here, we proposed simple approach integrate microstructured photonic fibers (MPFs) into wide bore capillaries 150 μm i.d.,...
As the society pays more and attention to environmental protection sustainable development of, green education has gradually become an important topic in field of education. This study addresses impact on secondary school students' thinking behavior depth. Using a questionnaire survey, this finds that formal schools helps motivate students pay extra for purchasing environmentally friendly products, reinforces idea "reuse, reuse many times". Informal establish "green living eco-friendly...
<title>Abstract</title> Mechanisms for resistance to cytotoxic cancer drugs are dependent on dynamic changes in the biochemistry of cellular pathways, information which is hard obtain at systems level. Here we use a deep functional proteomics implementation CETSA (Cellular Thermal Shift Assay) revealing range induced biochemical responses gemcitabine resistant and sensitive diffuse large B cell lymphoma (DLBCL) lines. Initial both, cells, reflect known targeted effects by ribonucleotide...
ABSTRACT While tremendous progress has been made in chemical proteomics for identifying protein-ligand interactions, it remains challenging proteome-wide identification of ligand-binding regions without modifying the ligands. Here, we discovered that “disruptive trypsinization” amplifies readout ligand-induced protein local stability shifts, and explored this notion developing “peptide-centric assay” (PELSA), a modification-free approach which achieves unprecedented sensitivity target...
Adverse health outcomes caused by environmental chemicals are often initiated via their interactions with proteins. Essentially, one chemical may interact a number of proteins and/or protein multitude chemicals, forming an intricate interaction network. Omics-wide protein-environmental profiling (PECI) is prominent importance for comprehensive understanding these networks, including the toxicity mechanisms action (MoA), and providing systematic safety assessment. However, such information...