A5064054665- Glycosylation and Glycoproteins Research
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Advanced Proteomics Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Infant Nutrition and Health
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Animal health and immunology
- Nutrition, Genetics, and Disease
- Science, Research, and Medicine
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Antimicrobial Peptides and Activities
- Protein Structure and Dynamics
- Microbial Natural Products and Biosynthesis
- Epigenetics and DNA Methylation
Dalian Institute of Chemical Physics
2023-2025
Chinese Academy of Sciences
2023-2025
Fudan University
2023-2024
Jiangnan University
2024
Jiangsu University
2024
Yixing People's Hospital
2024
Shanghai Institute of Pharmaceutical Industry
2023
University of Chinese Academy of Sciences
2023
Wayne State University
2016
Although the use of detergents in thermal proteome profiling (TPP) has become a common practice to identify membrane protein targets complex biological samples, surprisingly, there is no proteome-wide investigation into impacts detergent introduction on target identification performance TPP. In this study, we assessed TPP presence commonly used non-ionic or zwitterionic using pan-kinase inhibitor staurosporine, our results showed that addition either these significantly impaired at optimal...
Dysregulation of protein core-fucosylation plays a pivotal role in the onset, progression, and immunosuppression cancer. However, analyzing core-fucosylation, especially accurate determination (CF) site occupancy ratio, remains challenging. To address these problems, we developed truncation strategy that efficiently converts intact glycopeptides with hundreds different glycans into two truncated forms, i.e., monosaccharide HexNAc disaccharide HexNAc+core-fucose. Further combination...
Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial fermentation. However, the reliance on antibiotics production process leads to increased costs safety risks. To address these challenges, antibiotic-free Escherichia coli riboflavin producer was constructed using metabolic engineering approaches coupled with novel plasmid stabilization system. Initially, competitive pathways feedback inhibition...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
has developed multiple drug resistances, posing a significant threat to antibiotic efficacy. LysECD7, an endolysin derived from phages, could be promising therapeutic agent against multi-drug resistance
ABSTRACT While tremendous progress has been made in chemical proteomics for identifying protein-ligand interactions, it remains challenging proteome-wide identification of ligand-binding regions without modifying the ligands. Here, we discovered that “disruptive trypsinization” amplifies readout ligand-induced protein local stability shifts, and explored this notion developing “peptide-centric assay” (PELSA), a modification-free approach which achieves unprecedented sensitivity target...
Abstract The single-component colistin E2, with superior antibacterial activity and lower toxicity, was being developed as the latest generation of polymyxin drugs. However, E2 has not been tested quantitatively in biological matrices. In this study, based on quantitative detection methanesulphonate (CMS) by Zhao et al., 15 N-labeled used an internal standard (IS) for a more accurate CMS human plasma. A rapid ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS)...