A5102975743- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Plant Physiology and Cultivation Studies
- Circular RNAs in diseases
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Lignin and Wood Chemistry
- Plant Pathogens and Fungal Diseases
- Corruption and Economic Development
- RNA modifications and cancer
- Wood Treatment and Properties
- Pancreatic and Hepatic Oncology Research
- Biofuel production and bioconversion
- Dendrimers and Hyperbranched Polymers
Zhongda Hospital Southeast University
2017-2024
Dalian Institute of Chemical Physics
2021-2024
Chinese Academy of Sciences
2020-2024
University of Chinese Academy of Sciences
2021-2024
Beijing Academy of Agricultural and Forestry Sciences
2019-2024
South China Agricultural University
2022-2024
Ministry of Agriculture and Rural Affairs
2024
Fayetteville State University
2022-2023
University of Delaware
2023
Center for Innovation
2023
Exosomes play important roles in cell-cell communication, and are likely mediators of the metastatic cascade cancer. This study examined role exosomes pancreatic cancer cell adhesion, migration, invasion. We isolated purified from two isogenic lines with different potentials. Uptake highly Panc02-H7 cells decreased adhesion increased migration invasion capacity weakly Panc02 vitro. induced liver pre-metastatic niche formation naïve mice promoted primary tumor growth metastasis vivo....
A highly active CuZn–SiO2 catalyst with excellent stability in hydrogenation of methyl acetate (MA) was fabricated by the hydrolysis precipitation (HP) method. The HP method caused notable high dispersion both copper and zinc species due to strong interaction between metal silica support pseudo-homogeneous reaction during mixing process. According linear correlation TOF (Cu+) Zn/Cu ratio, Cu+ considered predominant site, site derived from has higher activity than those generated...
Hepatocellular carcinoma (HCC) is one of the most common malignancies with a poor response to chemotherapy. It very important identify novel therapeutic targets. Squalene epoxidase (SQLE), rate-limiting enzymes in cholesterol biosynthesis, recently has been found be involved tumorigenesis. However, its expression profile and function progression HCC remain largely unknown. Here, we that SQLE was upregulated tissues. Moreover, overexpression cells promoted cell proliferation migration, while...
The quantitative profiling of residue reactivity in proteins promotes the discovery covalent druggable targets for precise therapy. Histidine (His) residues, accounting more than 20% active sites enzymes, have not been systematically characterized their reactivity, due to lack labeling probes. Herein, we report a chemical proteomics platform site-specific analysis His by combination acrolein (ACR) and reversible hydrazine chemistry enrichment. Based on this platform, in-depth...
BACKGROUND:This study aimed to investigate the role of miRNA-339-5p in pancreatic cancer cell invasion and migration. MATERIAL AND METHODS:The differences between exosomal miRNAs PANC02 PANC02-H7 were studied by microarray analysis. We measured expression different groups; migration evaluated using Transwell wound healing assays relative proteins (E-cadherin, vimentin ZNF689) was WB assay. The correlation ZNF689 luciferase reporter gene RESULTS:Compared with exosome, analysis indicated that...
Abstract Aim Long noncoding RNAs (lncRNAs) are key mediators with a wide range of pathophysiological functions, but their role in human hepatocellular carcinoma (HCC) is still unclear. Methods An unbiased microarray study evaluated novel lncRNA, HClnc1, that linked to the development HCC. In vitro cell proliferation assays and an vivo xenotransplanted HCC tumor model were performed determine its followed by antisense oligo‐coupled mass spectrometry identify HClnc1‐interacting proteins. To...
Proteomics analysis of O-GalNAc glycosylation is important for the screening biomarkers and assessment therapeutic responses. However, its still faces challenges due to poor performance currently available enrichment methods. In this study, an method was established on basis Ti-IMAC(IV) materials, which could enrich intact glycopeptides via both hydrophilic interaction affinity interaction. This enabled nearly 200 identified from only 0.1 μL human serum. 2-fold different that HILIC method....
Objective: To investigate the expression of hsa_circ_0074298 (circular RNA) and molecular mechanism that promotes tumor growth enhances chemoresistance pancreatic cancer. Methods: Real-time reverse transcription-PCR was used to detect in The intracellular localization determined by RNA situ hybridization. CCK8 method, colony formation assay, Transwell flow cytometry were evaluate effects on proliferation, migration, invasion, cell cycle, apoptosis cancer cells. Bioinformatics analysis dual...
Arginine methylation is one of the most important post-translational modifications involved in regulation numerous biological processes. To better understand significance arginine methylation, enrichment methods need to be developed analyze methylated proteome at large-scale. Unfortunately, prevailing method based on immunoaffinity purification can only enrich a subset them due lack pan-specific antibodies. Therefore, it crucial develop stable and efficient antibody-free approach for global...
Recently, numerous efforts have been devoted to identifying drug targets and binding sites in complex proteomes, which is of great importance modern discovery. In this study, we developed a robust lysine reactivity profiling method systematically study drug-binding at the proteome level. This based on principle that specific region target proteins will change residues are located region, these changes can be detected with an enrichable reactive probe. Coupled data-independent acquisition...
The current study set out to investigate the role of long intergenic non-protein coding RNA (LINC) 00152 in pancreatic cancer (PC) cell glycolysis with microRNA (miR)-185-5p/Krüppel-like factor 7 (KLF7) axis.Firstly, PC tissues and cells as well control ones were collected from 53 patients, assessed for LINC00152 expression patterns.Besides, most differentially expressed selected further experiments.When was silenced or overexpressed, glucose consumption, lactic acid production, adenosine...
A common lenticel disorder which occurs in the peel of 'Xinli No. 7' pears (Pyrus bretschneideri Rehd.) had not previously been described. Symptoms this include enlarging and bulging lenticels results significant commercial losses. Understanding physiological basis developing practical methods to control it is crucial for successful marketing pear.The development was found be closely related endogenous ethylene production during storage. 1-Methylcyclopropene (1-MCP) combined with an...
We propose a novel pedagogic design to integrate teaching earnings management and accounting ethics at the undergraduate level. Earnings is an extensive topic related almost every in collegiate education. Generally Accepted Accounting Principles (GAAP) give accountants discretion exercise professional judgment on how report earnings. The ethicality of has its profound pedagogical challenges. This flexibility exposes many ethical dimensions for training. interaction offers particular...
<p id="p00010">Organic acid metabolites exhibit acidic properties. These serve as intermediates in major carbon metabolic pathways and are involved several biochemical pathways, including the tricarboxylic (TCA) cycle glycolysis. They also regulate cellular activity play crucial roles epigenetics, tumorigenesis, signal transduction. Knowledge of binding proteins organic is for understanding their biological functions. However, identifying these has long been a challenging task owing to...