A5033866713- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Tryptophan and brain disorders
- Peptidase Inhibition and Analysis
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Click Chemistry and Applications
- Biotin and Related Studies
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Antimicrobial Peptides and Activities
- Mass Spectrometry Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Biochemical and Structural Characterization
- Galectins and Cancer Biology
- Metabolism, Diabetes, and Cancer
- Advanced biosensing and bioanalysis techniques
- Infant Nutrition and Health
- Invertebrate Immune Response Mechanisms
Dalian Institute of Chemical Physics
2019-2025
Chinese Academy of Sciences
2019-2025
Changchun University of Chinese Medicine
2025
University of Chinese Academy of Sciences
2019-2024
Tang Du Hospital
2024
Air Force Medical University
2024
Ningbo Institute of Industrial Technology
2020-2023
Wenzhou Medical University
2023
Zhejiang University
2023
Shandong University
2014-2022
Abstract Glycopeptides with unusual glycans or poor peptide backbone fragmentation in tandem mass spectrometry are unaccounted for typical site-specific glycoproteomics analysis and thus remain unidentified. Here, we develop a tool, Glyco-Decipher, to address these issues. Glyco-Decipher conducts glycan database-independent matching exploits the pattern of shared backbones glycopeptides improve spectrum interpretation. We benchmark on several large-scale datasets, demonstrating that it...
Protein arginine methylation plays an important role in regulating protein functions different cellular processes, and its dysregulation may lead to a variety of human diseases. Recently, was found be involved modulating liquid–liquid phase separation (LLPS), which drives the formation membraneless organelles (MLOs). Here, we developed steric effect–based chemical-enrichment method (SECEM) coupled with liquid chromatography–tandem mass spectrometry analyze dimethylation (DMA) at proteome...
Abstract The lack of efficient biomarkers for the early detection gastric cancer (GC) contributes to its high mortality rate, so it is crucial discover novel diagnostic targets GC. Recent studies have implicated potential site‐specific glycans in diagnosis, yet challenging perform highly reproducible and sensitive glycoproteomics analysis on large cohorts samples. Here, a robust N‐glycoproteomics (HRN) platform comprising an automated enrichment method, stable microflow LC‐MS/MS system,...
Schizophrenia is a widespread and debilitating mental disorder. However, the underlying molecular mechanism of schizophrenia remains largely unknown no objective laboratory tests are available to diagnose this The aim present study was characterize alternations glucose metabolites identify potential diagnostic biomarkers for schizophrenia.Gas chromatography/mass spectrometry based targeted metabolomic method used quantify levels 13 in peripheral blood mononuclear cells (PBMCs) derived from...
Bottom-up proteomics has been increasingly applied in clinical research to study the disease pathophysiology and discover biomarkers. However, glycoproteomic analysis always requires tedious experimental steps for intact glycopeptide enrichment, which technique bottleneck large-scale of samples. Herein, we developed an automated enrichment method serum site-specific N-glycoproteome. This allowed processing one sample within 20 min. It showed higher specificity, more identifications, better...
Although thermal proteome profiling (TPP) acts as a popular modification-free approach for drug target deconvolution, some key problems are still limiting screening sensitivity. In the prevailing TPP workflow, only soluble fractions analyzed after treatment, while precipitate that also contain abundant information of drug-induced stability shifts discarded; sigmoid melting curve fitting strategy used data processing suffers from discriminations part human with multiple transitions. this...
Major depressive disorder (MDD) is a debilitating mood with various etiopathological hypotheses.
Abstract Evolutionary pressure drives gut microbiota–host coevolution and results in complex interactions between microbiota neural development; however, the molecular mechanisms by which governs host behavior remain obscure. Here, we report that colonization early life is crucial for to modulate brain development behavior; later or deletion of cannot completely reverse behaviors. Microarray analysis revealed an association absence expression cAMP responding element-binding protein (CREB)...
Both N-linked glycosylation and O-linked play essential roles in the onset progression of various diseases including cancer, N-/O-linked site-specific glycans have been proven to be promising biomarkers for discrimination cancer. However, micro-heterogeneity low abundance nature glycosylation, as well time-consuming tedious procedures enrichment intact glycopeptides, pose great challenges their efficient accurate characterization. In this study, we developed an integrated platform...
Proteostasis is fundamental for maintaining organismal health. However, the mechanisms underlying its dynamic regulation and how disruptions lead to diseases are largely unclear. Here, we conduct in-depth propionylomic profiling in Drosophila, develop a small-sample learning framework prioritize propionylation at lysine 17 of H2B (H2BK17pr) be functionally important. Mutating H2BK17 which eliminates leads elevated total protein level vivo. Further analyses reveal that H2BK17pr modulates...
Influenza is an acute respiratory infectious disease primarily transmitted through airborne droplets. The prevalence and spread of influenza viruses have significant impacts on global economic development public health. Current prevention control strategies for virus infections mainly rely vaccines antiviral drugs. However, vaccine efficacy limited by the antigenic drift mutation characteristics viruses, while drug resistance increasingly prevalent. Therefore, there urgent need novel agents....
The preparation of highly complex indole-fused polycyclics have been in high demand because their abundant biological activities. electrochemical coupling the radical intermediates with adjacent indole rings has...
Proteomics analysis of O-GalNAc glycosylation is important for the screening biomarkers and assessment therapeutic responses. However, its still faces challenges due to poor performance currently available enrichment methods. In this study, an method was established on basis Ti-IMAC(IV) materials, which could enrich intact glycopeptides via both hydrophilic interaction affinity interaction. This enabled nearly 200 identified from only 0.1 μL human serum. 2-fold different that HILIC method....
Titanium dioxide (TiO2) nanotubes are often used as carriers for loading materials such drugs, proteins, and growth factors.In this study, we loaded tetracycline onto TiO2 to demonstrate its antibacterial properties biocompatibility. The two-layered anodic with a honeycomb-like porous structure were fabricated by using two-step anodization, they simplified lyophilization method vacuum drying. Their physical properties, chemical compositions, wettability, surface morphologies of the different...