A5019530640- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Blood Coagulation and Thrombosis Mechanisms
- Ubiquitin and proteasome pathways
- Analytical chemistry methods development
- Catalysis and Oxidation Reactions
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Inflammatory Biomarkers in Disease Prognosis
- Advanced Breast Cancer Therapies
- Luminescence and Fluorescent Materials
- RNA and protein synthesis mechanisms
- Chemical Analysis and Environmental Impact
- Carbohydrate Chemistry and Synthesis
- Mesoporous Materials and Catalysis
- Catalytic Processes in Materials Science
- Photonic Crystals and Applications
- Chromatography in Natural Products
- Protein Kinase Regulation and GTPase Signaling
- Inhalation and Respiratory Drug Delivery
- Neonatal Respiratory Health Research
Zhengzhou University
2021-2024
China Pharmaceutical University
2023-2024
Dalian Institute of Chemical Physics
2015-2023
Chinese Academy of Sciences
2015-2022
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2021-2022
Wenzhou Medical University
2021-2022
University of Chinese Academy of Sciences
2015-2020
Entry Exit Inspection and Quarantine Bureau
2013
Selective enrichment and sensitive detection of phosphopeptides are great significance in many bioapplications. In this work, dendritic mesoporous silica nanoparticles modified with polydopamine chelated Ti4+ (denoted DMSNs@PDA-Ti4+) were developed to improve the selectivity phosphopeptides. The unique central-radial pore structures endowed DMSNs@PDA-Ti4+ a high surface area (362 m2 g–1), large volume (1.37 cm3 amount (75 μg mg–1). Compared conventional silica-based materials same...
Selective enrichment of glycopeptides from complex sample followed by cleavage N-glycans PNGase F to expose an easily detectable mark on the former glycosylation sites has become popular protocol for comprehensive glycoproteome analysis. On account high specificity, hydrazide chemistry based solid-phase extraction N-linked technique sparked numerous interests. However, enzymatic release captured beads through direct incubation with is not efficient due inherent steric hindrance effect. In...
Multisite phosphorylation of a protein, generally occurring in biological processes, plays important roles the regulation cellular functions. However, identification multi-phosphopeptides especially at low abundance is big challenge as extreme hydrophilicity and poor ionization efficiency multiphosphorylated peptides restrict deep inspection multisite processes. In this study, highly specific enrichment was achieved via modification dual-metal-centered zirconium-organic framework with...
Abstract Enrichment of glycopeptides by hydrazide chemistry (HC) is a popular method for glycoproteomics analysis. However, possible side reactions peptide backbones during the glycan oxidation in this have not been comprehensively studied. Here, we developed proteomics approach to locate such and found several types that could seriously compromise performance Particularly, HC failed identify N-terminal Ser/Thr because vicinal amino alcohol on these peptides generates aldehyde groups after...
Hierarchically porous materials have become a key feature of biological and been widely applied for adsorption or catalysis. Herein, we presented new approach to directly prepare phosphate-functionalized hierarchically hybrid monolith (HPHM), which simultaneously contained mesopores macropores. The design was based on the copolymerization polyhedral oligomeric vinylsilsesquioxanes (vinylPOSS) vinylphosphonic acid (VPA) by adding degradable polycaprolactone (PCL) additive. phosphate groups...
Albeit much less abundant than Ser/Thr phosphorylation (pSer/pThr), Tyr (pTyr) is considered as a hallmark in cellular signal transduction. However, its analysis at the proteome level remains challenging. The conventional immunopurification (IP) approach using antibodies specific to pTyr sites known have low sensitivity, poor reproducibility and high cost. Our recent study indicated that SH2 domain-derived pTyr-superbinder good replacement of antibody for enrichment peptides...
Cell surface is the primary site for sensing extracellular stimuli. The knowledge of transient changes on surfaceome upon a perturbation very important as initial changed proteins could be driving molecules some phenotype. In this study, we report fast cell labeling strategy based peroxidase-mediated oxidative tyrosine coupling strategy, enabling efficient and selective within seconds. With time 1 min, 2684 proteins, including 1370 (51%) surface-annotated (cell surface/plasma...
Tyrosine phosphorylation plays a major role in regulating cell signaling pathways governing diverse biological functions such as proliferation and differentiation. Systemically mapping phosphotyrosine (pTyr) sites is the key to understanding molecular mechanisms underlining pTyr-dependent signaling. Although mass spectrometry-based technologies have been widely used for pTyr site profiling quantification, their applications are often hindered by poor efficiency current multistep enrichment...
Clinical data analysis reveals that the expression of EphB4 receptor tyrosine kinase is significantly elevated in HER2-positive breast cancer and high levels strongly correlate with poor disease prognosis. However, impact activation on cells potential as a therapeutic target remain to be explored. Here, we show overexpression confers gain-of-function activities cells, rendering resistance HER2/EGFR inhibitor Lapatinib. Furthermore, using integrated transcriptomic phosphoproteomic analyses,...
Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins. Compared with glycoproteins, the glycoproteins are not well-studied due to their high heterogeneity low abundance. Herein, we presented novel chemoenzymatic method analyze core-1 type of O-GalNAcylation in human serum. In this approach, tryptic digest was first subjected PNGase F treatment release N-glycan then treated strong acid sialic residues from mucin-type O-glycans. way, internal Gal/GalNAc...
In this study, we presented an enrichment-free approach for the sensitive analysis of protein phosphorylation in minute amounts samples, such as purified complexes. This method takes advantage high sensitivity parallel reaction monitoring (PRM). Specifically, low confident phosphopeptides identified from data-dependent acquisition (DDA) data set were used to build a pseudotargeted list PRM allow identification additional with confidence. The development targeted is very easy same sample and...
In this study, we present a method to specifically capture phosphotyrosine (pTyr) peptides from minute amount of sample for the sensitive analysis protein tyrosine phosphorylation. We immobilized SH2 superbinder on monolithic capillary column construct microreactor enrich pTyr peptides. It was found that synthetic peptide could be enriched by mixture prepared spiking into tryptic digests α-casein and β-casein with molar ratios 1:1000:1000. The further applied pervanadate-treated HeLa cell...
A problem for "shot-gun" proteomics is that the peptides generated in proteolysis step overwhelm analytical capacity of current LC-MS/MS systems. straightforward approach to overcome this reduce sample complexity by isolating representative each protein. In study, we presented a facile solid-phase capture-release selectively enrich with N-terminal serine/threonine from protein digests. This method exploited highly efficient reaction between an aldehyde group and hydrazine group. The...
Purpose: To determine the prognostic significance of postoperative platelet/preoperative platelet ratio (PPR) in patients with operable non-small cell lung cancer (NSCLC), and assess its benefit compared to models relying solely preoperative counts (PLT). Materials Methods: A retrospective analysis 403 who underwent radical resection NSCLC our institution from 2013 2018 was conducted PLT PPR. Progression-free survival (PFS) overall (OS) were performed by Kaplan-Meier method. Single-factor...
Protein tyrosine phosphorylation (pTyr) plays a prominent role in signal transduction and regulation all eukaryotic cells. In conventional immunoaffinity purification (IP) methods, phosphotyrosine peptides are isolated from the digest of cellular protein extracts with phosphotyrosine-specific antibody identified by tandem mass spectrometry. However, low sensitivity, poor reproducibility, high cost universal concerns for IP approaches. this study, we presented an antibody-free approach to...