A5058082470- Circadian rhythm and melatonin
- Light effects on plants
- Neurobiology and Insect Physiology Research
- Endoplasmic Reticulum Stress and Disease
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
Huazhong Agricultural University
2023-2025
Huazhong University of Science and Technology
2016-2023
We report a database of circadian genes in eukaryotes (CGDB, http://cgdb.biocuckoo.org), containing ∼73 000 circadian-related 68 animals, 39 plants and 41 fungi. Circadian rhythm is ∼24 h behavioral physiological processes that exists almost all organisms on the earth. Defects system are highly associated with number diseases such as cancers. Although several databases have been established for rhythmically expressed genes, comprehensive cycling across phyla still lacking. From literature,...
Proteostasis is fundamental for maintaining organismal health. However, the mechanisms underlying its dynamic regulation and how disruptions lead to diseases are largely unclear. Here, we conduct in-depth propionylomic profiling in Drosophila, develop a small-sample learning framework prioritize propionylation at lysine 17 of H2B (H2BK17pr) be functionally important. Mutating H2BK17 which eliminates leads elevated total protein level vivo. Further analyses reveal that H2BK17pr modulates...
Ischemic stroke constitutes a multifaceted neurological affliction that spans various cellular types. Lack of dynamic chromatin accessibility data after is one the obstacles to understanding this process. To gain insights into variations in transcriptional regulation among cell types subsequent stroke, we employed single-nucleus ATAC-seq curate compendium from cerebral cortex mice subjected middle artery occlusion/reperfusion (MCAO/R). Tissue samples were collected at time points including...
The quality and efficiency of any PCR-based mutagenesis technique may not be optimal due to, among other things, amino acid bias, which means that the development efficient PCR-free methods is desirable. Here, we present a highly in vitro CRISPR/Cas9-mediated mutagenic (ICM) system allows rapid construction designed mutants manner. First, it involves plasmid digestion by utilizing complex Cas9 with specific single guide RNA (sgRNA) followed degradation T5 exonuclease to generate 15 nt...
Abstract Most organisms on the earth exhibit circadian rhythms in behavior and physiology, which are driven by endogenous clocks. Phosphorylation plays a central role timing clock, but how this contributes to overt is unclear. Here we conduct phosphoproteomics conjunction with transcriptomic proteomic profiling using fly heads. By developing pipeline for integrating multi-omics data, identify 789 (~17%) phosphorylation sites oscillations. We predict 27 potential kinases participate...
Abstract The circadian rhythm is an evolutionarily conserved mechanism with translational regulation increasingly recognized as pivotal in its modulation. In this study, we found that upstream open reading frames (uORFs) are enriched genes, particularly uORFs present core clock genes. We demonstrate evidence the of gene, Clock ( Clk ), rhythmically and substantially attenuate CLK protein translation Drosophila , pronounced suppression occurring during daylight hours. Eliminating results...