A5100438838- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Steroid Chemistry and Biochemistry
- Polyamine Metabolism and Applications
- Pharmacogenetics and Drug Metabolism
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Plant biochemistry and biosynthesis
- Microbial Metabolism and Applications
- Hormonal Regulation and Hypertension
- Microbial Metabolites in Food Biotechnology
- GABA and Rice Research
- RNA and protein synthesis mechanisms
- Acute Lymphoblastic Leukemia research
- Microbial metabolism and enzyme function
- Ginkgo biloba and Cashew Applications
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Wastewater Treatment and Nitrogen Removal
Jiangnan University
2016-2025
Guangxi Zhuang Autonomous Region Brain Hospital
2024
Yixing People's Hospital
2023-2024
Jiangsu University
2023-2024
Hefei University of Technology
2024
Wuhan University
2002-2024
Zhongnan Hospital of Wuhan University
2022-2024
Hebei Agricultural University
2024
State Key Laboratory of Natural Medicine
2020-2023
China Pharmaceutical University
2016-2023
Forty-five bacterial strains that produced diffusive pigments were isolated from 40 soil samples. Maximum pigment production was a Streptomyces kathirae strain designated SC-1. The diffused characterized by UV–visual and infrared spectroscopy, MS 1H nuclear magnetic resonance imaging, confirmed as melanin. This may be the first report of melanin S. kathirae. To enhance production, culture medium optimized conducting series batch fermentations in defined medium, results analysed statistically...
Abstract In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3‐butanediol (2,3‐BD) designated as B10‐127 was isolated in our lab. The produced 2,3‐BD effectively under condition 20% glucose (quality concentration), showed high‐glucose tolerance. effects initial concentration, temperature, pH and agitation on production were investigated this work proper parameters identified. Accordingly, fed‐batch culture larger scales (5 l)...
BackgroundPreviously, a safe strain, Bacillus amyloliquefaciens B10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD). However, B. yields large quantities acetoin, lactate and succinate by-products, the 2,3-BD yield remains prohibitively low commercial production. Methodology/Principal FindingsIn metabolic pathway, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes conversion 3-phosphate glyceraldehyde to...
Abstract L-arginine is an important amino acid in food and pharmaceutical industries. Until now, the main production method of China highly polluting keratin hydrolysis. The industrial level by microbial fermentation has become task. In previous work, we obtained a new producing Corynebacterium crenatum (subspecies glutamicum ) through screening mutation breeding. this performed systems pathway engineering C. for improved production, involving amplification biosynthetic flux removal feedback...
NAD
Bacillus amyloliquefaciens B10-127 exhibited an excellent ability for industrial-scale microbial fermentation of 2,3-butanediol (2,3-BD) from biodiesel-derived glycerol. However, the accumulation by-products (acetoin, acetoin, lactate and succinate) 2,3-BD yield remains prohibitively low commercial production. Several strategies were developed to manipulate carbon flux branch in a designed B. amyloliquefaciens. Firstly, extra copies NADH/NAD+ regeneration system introduced into by...
Bacillus subtilis produces acetoin as a major product along with several NADH-dependent byproducts, especially 2,3-butanediol. In this study, the down-regulation of NADH pool and redistribution NADH/NADPH were targeted using external genetic processes, means by which to redistribute metabolic flux in favor synthesis. First, it was found that use carbon sources different oxidation states resulted very intracellular NADH/NAD(+) ratios dictated total process yield acetoin. A mixture glucose...
With the increasing use of animal and plant proteins, pollution due to nitrogen sources is attracting attention. In particular, amount nitrogen-containing sewage discharged into environment has increased significantly, causing eutrophication water bodies environmental degradation quality. Traditionally, nitrifying bacteria perform ammonia nitrification under aerobic conditions, while denitrifying nitrate/nitrite denitrification anaerobic conditions. However, heterotrophic microorganisms...
ε-poly-l-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by Streptomyces albulus, but the production wild-type strains fails to meet demands of industrialization. To address this issue, adaptive laboratory evolution (ALE) was successfully employed in study, subjecting S. albulus CICC 11022 environmental stresses such as acidic pH and antibiotics (rifampicin, gentamicin, streptomycin). As result ALE, an evolutionary strain C214 obtained,...
Acetoin, a promising bio-based platform chemical, is mainly produced through chemical synthesis. Given the increasing attention to nonrenewable resources, developing safe and efficient microbial technologies for acetoin production necessary. This study redirected more carbon flux synthesis by deleting nonessential functional genes in Bacillus subtilis. Subsequently, based on spatial modulation engineering, biological regulatory elements DNA scaffold were used enhance co-catalytic capacity of...
Surface-displayed whole-cell biocatalysis offers significant advantages over traditional intracellular enzyme-based methods but faces challenges in complex multienzyme processes and low efficiency. We present Scaffold-Mediated Assembly Regulation Tool (SMART), a programmable platform for dynamic control of biocatalysts on Corynebacterium glutamicum surfaces. SMART integrates orthogonal cross-linked scaffolds with the twin Catcher/Tag pairs (SpyCatcher/SpyTag SnoopCatcher/SnoopTag),...
Menaquinone-7 (MK-7) plays a crucial role in preventing fractures and certain cardiovascular diseases is one of the essential vitamins human body. In this study, strain Bacillus subtilis that produces MK-7 was isolated from commercially available natto fermentation agents, with an titer 75 mg/L. It named L-5. Firstly, by employing Atmospheric Room Temperature Plasma (ARTP) mutagenesis technology protoplast fusion techniques, mutants resistant to 1-hydroxy-2-naphthoic acid (HNA) diphenylamine...
D-tagatose is an ideal sucrose substitute with potential applications in food and healthcare. The combined catalysis of polyphosphate kinase (PPK), fructose (FRK), D-tagatose-6-phosphate 3-differential anisomerase (FbaA) phytase provides a low-cost convenient pathway for the biosynthesis from D-fructose; however, there still problem low catalytic efficiency that needs to be solved urgently. Therefore, this study enhanced by optimizing expression levels PPK, FRK FbaA polycistronic system...
Acetoin (3-hydroxy-2-butanone), an extensively-used food spice and bio-based platform chemical, is usually produced by chemical synthesis methods. With increasingly requirement of security environmental protection, bio-fermentation acetoin microorganisms has a great promising market. However, through metabolic engineering strategies, the mixed acid-butanediol fermentation metabolizes certain portion substrate to by-products organic acids such as lactic acid acetic acid, which causes energy...