A5055792676- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Food Quality and Safety Studies
- Analytical Chemistry and Sensors
- Microbial Metabolism and Applications
- Synthesis and properties of polymers
- Biochemical Acid Research Studies
- Electrochemical sensors and biosensors
- Biotin and Related Studies
- Microbial Metabolites in Food Biotechnology
- Epoxy Resin Curing Processes
- GABA and Rice Research
- Morinda citrifolia extract uses
- Microbial metabolism and enzyme function
- Neurological diseases and metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Cocoa and Sweet Potato Agronomy
- Biochemical and biochemical processes
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
Zhejiang University of Technology
2022-2025
Northeast Agricultural University
2023-2025
Chinese People's Liberation Army
2025
Spice and Beverage Research Institute
2024
Chinese Academy of Tropical Agricultural Sciences
2024
Auburn University
2020-2021
Jiangnan University
2014-2021
Shandong University
2015-2018
Southwest Petroleum University
2018
Zhejiang University
2011
Abstract Fermentation is crucial for inducing desirable flavor and aroma profiles in cocoa products. This research focused on identifying microbial strains isolated from spontaneous fermentation Hainan through 16S Internal Transcribed Spacer (ITS) sequencing. Pectinase activity was screened, metabolic dynamics of sugars organic acids were analyzed using high‐performance liquid chromatography. Additionally, gas chromatography‐mass spectrometry employed the quantification volatile compounds....
Abstract Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it still challenging to generate microbial strains that can produce target biochemical high levels. Here, we report example biosynthesis high-value and easy-recoverable derivatives built upon natural pathways, leading improvement in bioproduction efficiency. By leveraging pathways solventogenic clostridia co-producing acyl-CoAs, acids alcohols...
l-Homoserine is a promising C4 platform compound used in the agricultural, cosmetic, and pharmaceutical industries. Numerous works have been conducted to engineer
NAD
O-acetyl-L-homoserine (OAH) is a promising platform compound for the production of L-methionine and other valuable compounds, while its low yield conversion rate limit industrial application. To solve these problems, we constructed strain high OAH with previously L-homoserine producer Escherichia coli HS33 as chassis by systematic metabolic engineering. Firstly, PEP accumulation, pyruvate utilization, synthesis pathway (overexpressing aspB, aspA, thrAC1034T) were enhanced to obtain an...
Optically pure 1,2-amino alcohols are highly valuable products as intermediates for chiral pharmaceutical products. Here we designed an environmentally friendly non-natural biocatalytic cascade efficient synthesis of from cheaper epoxides. A redesignated ω-transaminase PAKω-TA was tested and showed good bioactivity at a lower pH than other reported transaminases. The efficiently constructed single one-pot E. coli recombinant, by coupling SpEH (epoxide hydrolase), MnADH (alcohol...
The objective of this study was to reveal the microbial succession and metabolic characteristics during traditional fermentation Hainan Shanlan rice wine (HSRW). Correlations between core microorganisms key metabolites were explored. Four bacterial genera (Pediococcus, Levilactobacillus, Pantoea Limosilactobacillus) 3 fungal (Saccharomyces, Rhizopus, Saccharomycopsis) dominant in HSRW. Alcohols, esters organic acids main 2-Ethyl-1-hexanol, dimethylsilanediol, pentaethylene glycol, ethyl...
Unnatural amino acids (UAAs) play a key role in modern medicinal chemistry such as small molecules and peptide-based drugs with fast-growing markets. Low efficiency for natural enzymes including leucine dehydrogenase (LeuDH, EC1.4.1.9) are one major challenge UAA production. Here, rational engineering of LeuDH from Bacillus cereus structure-based design approach is studied. The results achieve higher enzymatic activity stability toward α-keto acid reduction by improving the hydrophobic...
Abstract A mesoporous ZnO/carbon composite is designed for coimmobilization of two oxidoreductases involving a novel “kiwifruit‐assembly” pattern. The ( S )‐carbonyl reductase II‐glucose dehydrogenase on nanoparticles (SCRII–GDH nano ) exhibits 40–50% higher specific activity than the free enzyme and significantly improves stabilities enzymes to heat, pH solvents. It performs asymmetric catalysis 75 × 10 −3 m substrate with perfect yield 100% an excellent enantioselectivity 99.9% within 1 h....