A5005959464- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Geological and Geochemical Analysis
- Amino Acid Enzymes and Metabolism
- Geochemistry and Geologic Mapping
- Pancreatic function and diabetes
- High-pressure geophysics and materials
- Pharmacogenetics and Drug Metabolism
- Hydrocarbon exploration and reservoir analysis
- earthquake and tectonic studies
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Steroid Chemistry and Biochemistry
- HIV Research and Treatment
- Carbohydrate Chemistry and Synthesis
- Chemical Reactions and Isotopes
- Metabolism and Genetic Disorders
- Cancer Research and Treatments
- Epoxy Resin Curing Processes
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
Jiangnan University
2015-2024
Henan Institute of Geological Survey
2016-2024
The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine
2024
Guangxi University of Chinese Medicine
2024
Shanghai East Hospital
2024
China University of Geosciences (Beijing)
2014-2024
Harbin Medical University
2022-2024
First Affiliated Hospital of Harbin Medical University
2022-2024
Daqing Oilfield General Hospital
2023
Johns Hopkins Medicine
2021
The exact role magmatic versus metasomatic processes play in Ta-Nb mineralization is a question that has been debatable for several decades. Jiabusi deposit chosen to address this question, its ample endowments of variety oxides and mica the various rock types could provide insights into whole evolutional history ore-forming during magmatic-hydrothermal evolution. Textural chemical evidence suggests Fe-mica granite pegmatitic portion magmatic, indicating co-precipitated columbite-group...
Structure-guided design of substrate-binding pocket inversed the stereoselectivity an NADH-dependent medium-chain alcohol dehydrogenase (MDR) from Prelog to anti-Prelog. The pocket-forming amino acids, especially unconserved residues as hotspots, play critical roles in directing MDRs' stereoselectivity.
Acetoin (3-hydroxy-2-butanone), an extensively-used food spice and bio-based platform chemical, is usually produced by chemical synthesis methods. With increasingly requirement of security environmental protection, bio-fermentation acetoin microorganisms has a great promising market. However, through metabolic engineering strategies, the mixed acid-butanediol fermentation metabolizes certain portion substrate to by-products organic acids such as lactic acid acetic acid, which causes energy...
We have developed a carbonyl group-dependent colorimetric method for assay of reductases using inexpensive 2,4-dinitrophenylhydrazine (DNPH).
Experiments were designed to determine if the effect of preload on spontaneous contractile activity rat mesenteric lymphatics would be different under isometric and isobaric conditions.Isobaric protocols cannulated, pressurized ( approximately 120 microm, ID) measured effects lumenal pressure contraction amplitude, frequency, rate diameter change (dD/dt). Analogous conducted using a wire myograph passive force (preload) characteristics spontaneous, transients. Servo-control systems allowed...
A short-chain carbonyl reductase (SCR) from Candida parapsilosis catalyzes an anti-Prelog reduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol (PED) and exhibits coenzyme specificity for NADPH over NADH. By using site-directed mutagenesis, the mutants were designed with different combinations Ser67Asp, His68Asp, Pro69Asp substitutions inside or adjacent binding pocket. All mutations caused a significant shift enantioselectivity toward (R)-configuration during reduction. The...
A novel short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) from Candida parapsilosis exhibits coenzyme specificity for NADPH over NADH. It catalyzes an anti-Prelog type reaction to reduce 2-hydroxyacetophenone into (S)-1-phenyl-1,2-ethanediol. The coding gene was overexpressed in Escherichia coli and the purified protein crystallized. crystal structure of apo-form solved 2.7 resolution. This forms a homo-tetramer with broken 2-2-2 symmetry. overall fold each SCR subunit is similar...
Background: Erythritol is a zero-calorie sweetener that widely used in the food, pharmaceutical, and medical industries. Crude glycerol main by-product of biodiesel, effective utilization crude will help to improve biodiesel viability. Previous studies on production erythritol from Y. lipolytica using as carbon source have focused optimizing fermentation process mutant Wratislavia K1, while metabolic engineering has not been successfully applied. Results: To this end, we engineered yeast...
Abstract A mesoporous ZnO/carbon composite is designed for coimmobilization of two oxidoreductases involving a novel “kiwifruit‐assembly” pattern. The ( S )‐carbonyl reductase II‐glucose dehydrogenase on nanoparticles (SCRII–GDH nano ) exhibits 40–50% higher specific activity than the free enzyme and significantly improves stabilities enzymes to heat, pH solvents. It performs asymmetric catalysis 75 × 10 −3 m substrate with perfect yield 100% an excellent enantioselectivity 99.9% within 1 h....
l -Threonine aldolases (TAs) can catalyze aldol condensation reactions to form β-hydroxy-α-amino acids, but afford unsatisfactory conversion and poor stereoselectivity at the C β position. In this study, a directed evolution coupling high-throughput screening method was developed screen more efficient -TA mutants based on their activity. A mutant library with over 4000 from Pseudomonas putida were obtained by random mutagenesis. About 10% of retained activity toward...