A5101999093- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Antibiotic Resistance in Bacteria
- RNA modifications and cancer
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- Biochemical and Structural Characterization
- Neuroscience and Neuropharmacology Research
- Renal and Vascular Pathologies
- Fungal and yeast genetics research
- Gynecological conditions and treatments
- Cardiomyopathy and Myosin Studies
- Nerve Injury and Rehabilitation
- Endometrial and Cervical Cancer Treatments
- NF-κB Signaling Pathways
- Antimicrobial Peptides and Activities
- Vascular anomalies and interventions
- Adrenal and Paraganglionic Tumors
- bioluminescence and chemiluminescence research
- Particle accelerators and beam dynamics
- Microbial Natural Products and Biosynthesis
Columbia University
2019-2024
Fairchild Semiconductor (United States)
2020-2023
Shiv Nadar University
2023
New York Structural Biology Center
2023
National Institute of Immunology
2012-2019
National Dairy Research Institute
2019
Institute of Microbial Technology
2013-2018
Vardhman Mahavir Medical College & Safdarjung Hospital
2017
King George's Medical University
2017
University of Allahabad
2017
Not your typical GPCR Among the large family of G protein–coupled receptors (GPCRs) are many orphans, so called because their signaling reactions remain poorly understood. these is GPR158 which highly expressed in nervous system and implicated processes from cognition to memory mood. Patil et al . determined a high-resolution structure alone bound regulator protein (RGS) complex. has an unusual dimerization mode with extensive interaction interface that locks it conformation likely prevents...
Mitochondria are valuable subcellular organelles and play crucial roles in redox signaling living cells. Substantial evidence proved that mitochondria one of the critical sources reactive oxygen species (ROS), overproduction ROS accompanies imbalance cell immunity. Among ROS, hydrogen peroxide (H2O2) is foremost regulator, which reacts with chloride ions presence myeloperoxidase (MPO) to generate another biogenic molecule, hypochlorous acid (HOCl). These highly primary cause damage DNA...
Abstract Multiple paralogous ABCF ATPases are encoded in most genomes, but the physiological functions remain unknown for of them. We herein compare four Escherichia coli K12 ABCFs – EttA, Uup, YbiT, and YheS using assays previously employed to demonstrate EttA gates first step polypeptide elongation on ribosome dependent ATP/ADP ratio. A Δ uup knockout, like ettA , exhibits strongly reduced fitness when growth is restarted from long-term stationary phase, neither ybiT nor yheS this...
GPR155 is a polymodal lysosomal membrane transceptor comprising both transporter domain and GPCR domain, predominantly expressed in brain. facilitates cholesterol-dependent mTORC1 signaling implicated neurological disorders like Huntington disease. The likely does not bind extracellular ligands canonically, the functional relationship between domains remains unclear. Here, we report first structures of monomeric human two distinct dimers, revealing an inward-open inactivated with unique...
The genomes of most mesophilic organisms encode multiple A TP- B inding C assette F (ABCF) proteins. EttA, one four E. coli paralogs, regulates synthesis the first peptide bond on ribosome dependent ATP/ADP ratio, while ntibiotic Re sistance factors (AREs), paralogs in other organisms, both regulate and directly mediate resistance to ribosome-targeted antibiotics. However, physiological functions remain unclear for mechanism-of-action has yet be rigorously established any paralog. We herein...
Structural genomics consortia established that protein crystallization is the primary obstacle to structure determination using x-ray crystallography. We previously demonstrated propensity systematically related sequence, and we subsequently performed computational analyses showing arginine most overrepresented amino acid in crystal-packing interfaces Protein Data Bank. Given similar physicochemical characteristics of lysine, hypothesized multiple lysine-to-arginine (KR) substitutions should...
Abstract This is the first report describing temperature based initiation of gelsolin’s F-actin depolymerization activity, even in absence free Ca 2+ or low pH. Small angle X-ray scattering (SAXS) and circular dichroism (CD) studies revealed that range 30–40 °C capable opening G1 domain alone, as remaining domains are held together by -sensitive C-tail latch without any loss secondary structural content. Full all tail-less gelsolin, retention closed shape for G2–G6 gelsolin merely heating,...
ABSTRACT Cystic fibrosis (CF) is caused by mutations in a chloride channel called the human Fibrosis Transmembrane Conductance Regulator (hCFTR). We used cryo-EM global conformational ensemble reconstruction to characterize mechanism which breakthrough drug VX445 (Elexacaftor) simultaneously corrects both protein-folding and channel-gating defects CF mutations. drives hCFTR molecules harboring gating-defective G551D mutation towards open-channel conformation binding site first transmembrane...
Precise synapse formation is essential for normal functioning of the nervous system. Retinal photoreceptors establish selective contacts with bipolar cells, aligning neurotransmitter release apparatus postsynaptic signaling cascades. This involves transsynaptic assembly between dystroglycan-dystrophin complex on photoreceptor and orphan receptor GPR179 cell, which mediated by extracellular matrix protein pikachurin (also known as EGFLAM). plays a critical role in synaptic organization signal...
Protein dendrimers made easy: We present a chemoenzymatic strategy for the synthesis of well-defined protein in which proteins orthogonally labelled by sortase-mediated ligation are conjugated to dendritic scaffold azide–alkyne cycloaddition. This "sortase-click" uses readily accessible His6-tagged and can serve as powerful method covalent assembly multivalent proteins.
Here, we report that minimal functional gelsolin i.e. fragment 28-161 can display F-actin depolymerizing property even after heating the protein to 80 °C. Small angle X-ray scattering (SAXS) data analysis confirmed under Ca2+-free conditions, associates into monomer dimer and tetramer, which later forms β-amyloids, but in presence of Ca2+, it dimers proceed non-characterizable aggregates. The dimeric association also explained observed decrease ellipticity circular dichroism experiments with...
Abstract Regulation of macrophage PCD plays an important role in pathogenesis leishmaniasis. However, the precise involvement any parasite molecule this process remains uncertain. In current study, silico wide analysis demonstrated that genes Leishmania donovani genome are highly enriched for CpG motifs, with sequence frequency 8.7%. Here, we show unmethylated species-specific motifs LdDNA significantly (P = 0.01) delay by endosomal interaction TLR9 via adaptor protein MyD88. Importantly,...
Multivalent proteins or protein dendrimers are useful for clinical and biotechnological applications. However, assembly of chemically defined is a challenging endeavor. In the past, majority have been developed on branched lysine scaffolds usually limited to valency two four. The naturally occurring cyclodextrin (CD) scaffold composed 6–8 glucose units offers possibility expanding valency. Here we adapted chemoenzymatic-click strategy displaying heptavalent peptides large β-cyclodextrin...
Toll/IL-1R (TIR) domain, that is, the cytoplasmic in toll-like receptors (TLRs) from different species showed high sequence conservation stretches spread across surface as well core of domain. To probe structure–function significance these residues, especially those coming TIR domains, we analyzed molecular dynamics trajectories similarity based models human domains. This study brought forth N-terminal domain simultaneously interacts with flanking residues BB loop and central β-sheets. At...
Structural genomics consortia established that protein crystallization is the primary obstacle to structure determination using x-ray crystallography. We previously demonstrated propensity systematically related sequence, and we subsequently performed computational analyses showing arginine most overrepresented amino acid in crystal-packing interfaces Protein Data Bank. Given similar physicochemical characteristics of lysine, hypothesized multiple lysine-to-arginine (KR) substitutions should...
Superior mesenteric artery (SMA) injury is a rare event during abdominal surgery. We report the first case of inadvertent superior surgery large malignant adrenocortical tumor with inferior vena cava thrombus. The cause was anatomical distortion great vessels due to massive nature tumor. managed successfully by immediate end-to-end anastomosis artery.
Portal hypertension is one of the most mystifying and disconcerting abdominal ailment. Ultrasonography (USG) an effective diagnostic tool for its prompt management. Knowledge normal calibre portal vein in a local setting essential as literature reports contrasting values different regions. It helps early diagnosis even before it clinically manifested thereby assisting clinicians interventional radiologists pertinent management.Study was aimed to evaluate Vein Diameter (PVD) find correlation...
microRNA-29b (miR-29b) plays an important role in controlling DNA methylation cells. We investigated its during early embryonic development buffalo embryos produced by somatic cell nuclear transfer (SCNT) and vitro fertilization (IVF). miR-29b expression was highest at the 2-cell stage, decreased (p < 0.001) 4-cell remained low thereafter 8-cell, morula, blastocyst stages, showing a similar pattern cloned IVF embryos. Treatment of reconstructed with mimic for 1 hour after electrofusion...
The brachial plexus consists of a network nerves that innervates the upper limbs and its musculature.We report rare formation lateral cord observed during dissection 47-year-old male cadaver at Department Anatomy, Vardhman Mahavir Medical College, New Delhi, India, in 2016.The was exceptionally long with twin pectoral roots median nerve.The proximal root nerve thin comparison to medial distal thicker followed an unusual course through coracobrachialis muscle.In lower third arm, artery-along...
Objective: Though the adverse effects of pesticides used in agriculture may not immediately be visible human population however its long term exposure cause detrimental by biomagnifications and bioaccumulation. Nowadays bioinformatics serves as an silico tool only for homology alignment but also prediction quaternary structures biochemicals. The present study was aimed to compare potential toxicities triazophos chlorpyrifos (organophosphates; OPs) cypermethrin deltamethrin (pyrethroids)...