A5030272776- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Pneumocystis jirovecii pneumonia detection and treatment
- Glycosylation and Glycoproteins Research
- Bacterial Infections and Vaccines
- T-cell and B-cell Immunology
- Diagnosis and treatment of tuberculosis
- Inhalation and Respiratory Drug Delivery
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Mast cells and histamine
- Drug Transport and Resistance Mechanisms
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Asthma and respiratory diseases
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Protein purification and stability
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Antibiotic Resistance in Bacteria
- Botanical Research and Chemistry
National Institute of Immunology
2011-2025
RELX Group (Netherlands)
2023
National Institute of Science, Technology and Development Studies
2010
National Institute of Allergy and Infectious Diseases
1998-2004
National Institutes of Health
1998-2004
International Institute of Molecular and Cell Biology
2001
Uppsala University
2001
Jawaharlal Nehru University
1991-1996
Streptococcus pneumoniae (pneumococcus) is a major bacterial pathogen that causes pneumonia and septicemia in humans. Pneumococci are cleared from the host primarily by antibody dependent opsonophagocytosis phagocytes like neutrophils. Neutrophils release neutrophil extracellular traps (NETs) on contacting pneumococci. NETs immobilize pneumococci restrict its dissemination host. One of strategies utilized to evade immune response involves use DNase(s) degrade NETs. We screened secretome...
Streptococcus pneumoniae is a leading cause of bacterial pneumonia, sepsis and meningitis. Surface accessible proteins S. are being explored for the development protein-based vaccine in order to overcome limitations existing polysaccharide-based pneumococcal vaccines. To identify potential candidate, we resolved surface-associated TIGR4 strain using two-dimensional gel electrophoresis followed by immunoblotting with antisera generated against whole heat-killed TIGR4. Ten immunoreactive spots...
Abstract Lineage trees of mutated rearranged Ig V region sequences in B lymphocyte clones often serve to qualitatively illustrate claims concerning the dynamics affinity maturation. In this study, we use a novel method for analyzing lineage tree shapes, using terms from graph theory quantify differences between primary and secondary diversification rabbits chickens. these species, gene starts with rearrangement single (in chicken) or few rabbit) VH genes. Somatic hypermutation conversion...
Immunization of human volunteers with a single dose pneumococcal surface protein A (PspA) stimulates broad cross-reactive Abs to heterologous PspA molecules that, when transferred, protect mice from fatal infection Streptococcus pneumoniae. In this study, we report the molecular characterization 36 mouse mAbs generated against extracellular domain (PspA(3-286)) strain R36A. PspA(3-286) were encoded by diverse V(H) and V(kappa) families/genes. The H chain CDR3 L lengths 3-13 (7.8 +/- 0.5) 8-9...
The emergence of new variants SARS-CoV-2 necessitates unremitting efforts to discover novel therapeutic monoclonal antibodies (mAbs). Here, we report an extremely potent mAb named P4A2 that can neutralize all the circulating concern (VOCs) with high efficiency, including highly transmissible Omicron. crystal structure Fab:RBD complex revealed residues RBD interact are a part ACE2-receptor-binding motif and not mutated in any VOCs. pan coronavirus pseudotyped neutralization assay confirmed is...
The young rabbit appendix is a dynamic site for primary B cell repertoire development. To study diversification patterns during clonal expansion, we collected single cells from 3- to 9-wk-old rabbits and sequenced rearranged H L chain genes. Single obtained by hydraulic micromanipulation or laser capture microdissection were lysed, PCR amplified, products directly sequenced. Gene conversion-like changes occurred in sequences 3-4 wk of age. Somatic mutations found the D regions that lack...
Protein dendrimers made easy: We present a chemoenzymatic strategy for the synthesis of well-defined protein in which proteins orthogonally labelled by sortase-mediated ligation are conjugated to dendritic scaffold azide–alkyne cycloaddition. This "sortase-click" uses readily accessible His6-tagged and can serve as powerful method covalent assembly multivalent proteins.
The young rabbit appendix and the chicken bursa of Fabricius are primary lymphoid organs where B cell Ab repertoire develops in germinal centers (GCs) mainly by a gene conversion-like process. In human mouse, V-gene diversification somatic hypermutation GCs secondary leads to affinity maturation. We asked whether conversion, hypermutation, or both occur splenic during responses hapten DNP. determined DNA sequences rearranged heavy light chain V region segments single cells from developing...
Abstract We used PCR to amplify rearranged VHDJHgenes in single cells collected by micromanipulation from splenic germinal centers of immunized adult rabbits. In the course study, objective which was analyze diversification VHDJH sequences, we were surprised find 7 and 10 days after immunization with VH1a2 as well a-negative (y33 x32) sequences that identical or close germline (10 fewer changes). About 58% (82/140) had unique CDR3 regions unrelated. seven different centers, found one four...
ABSTRACT The shortcomings of the licensed polysaccharide-based pneumococcal vaccine are driving efforts toward development a protein-based that is serotype independent and effective in all age groups. An opsonophagocytic killing assay (OPKA) used to evaluate antibody response against vaccines. However, OPKA not reliable for noncapsular antigens. Thus, there need develop an vitro surrogate protection protein candidates like surface antigen A (PspA). PspA serologically variable cell virulence...
Bacterial capsular polysaccharides are components of many modern vaccines, but they weakly immunogenic. Herein, we describe the delivery pneumococcal polysaccharide serotype-1 (PCP-1) in polylactide polymeric particles to enhance its immunogenicity. Immunization with PCP-1-entrapped elicited long-term memory antibody responses from a single intramuscular injection. nanoparticles (NPs) significantly higher anti-PCP-1 IgG than that observed soluble and microparticles (MPs) formulations....
Abstract Glycoconjugate vaccines elicit robust anti-polysaccharide Ab response by recruiting T-cell help. Multiple doses of glycoconjugate vaccine are required to induce long-lasting immunity. The characteristics have been reported previously. However, the effect booster immunization on and anti–carrier protein repertoire remains poorly understood. In this study, we used clinically relevant pneumococcal capsular polysaccharide type 14 (PCP14) conjugated with cross-reactive material 197...
The mechanisms described here account for development of the heterogeneous high-affinity anti-DNP antibodies that rabbits can produce. Rearranged immunoglobulin light and heavy chain genes from single DNP-specific splenic germinal center B cells were amplified by PCR. We found in clonal lineages, rearranged V[kappa] V[H] are further diversified gene conversion somatic hypermutation. positive negative selection amino acids complementarity-determining regions observed allows emergence a...