A5002686740- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Cervical Cancer and HPV Research
- Reproductive Physiology in Livestock
- Protein purification and stability
- Genetic and phenotypic traits in livestock
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Vibrio bacteria research studies
- Cell death mechanisms and regulation
- Tuberculosis Research and Epidemiology
- Animal Virus Infections Studies
- Birth, Development, and Health
- Mycobacterium research and diagnosis
- Advanced Biosensing Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Respiratory viral infections research
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Hepatitis Viruses Studies and Epidemiology
Translational Health Science and Technology Institute
2015-2024
Chhatrapati Shahu Ji Maharaj University
2022-2024
All India Institute of Medical Sciences
2005-2016
Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan
2015
There is a desperate need for safe and effective vaccines, therapies, diagnostics SARS- coronavirus 2 (CoV-2), the development of which will be aided by discovery potent selective antibodies against relevant viral epitopes. Human phage display technology has revolutionized process identifying optimizing antibodies, providing facile entry points further applications. Herein, we use this to search targeting receptor-binding domain (RBD) CoV-2. Specifically, screened naïve human semisynthetic...
Mitochondrial DNA (mtDNA) is known for high mutation rates caused by lack of protective histones, inefficient repair systems, and continuous exposure to mutagenic effects oxygen radicals. Alterations in the non-coding displacement (D) loop mitochondrial are present many cancers. It has been suggested that extent mutations might be useful prognosis cancer outcome and/or response certain therapies. In order investigate whether a incidence exist cervical patients, we examined frequency D-loop...
Human papilloma virus is a causative factor in the etiology of cervical cancer with HPV16 being most prevalent genotype associated it. Intratype variations oncogenic E6/E7 and capsid L1 proteins HPV 16 besides phylogenetic importance, are risk viral persistence progression. The objective this multicentric study was to identify HPV-16 E6, E7 variants India their possible biological effects. Squamous cell biopsies were collected from 6 centres examined for presence 16. Variants characterized...
Cervical cancer is the second most common cause of in women and has a high mortality rate. Cisplatin, an antitumor agent, generally used for its treatment. However, administration cisplatin associated with side effects intrinsic resistance. Morinda citrifolia (Noni), natural plant product, been shown to have anti-cancer properties. In this study, we Noni, cisplatin, two combination study their cytotoxic apoptosis-inducing cervical HeLa SiHa cell lines. We demonstrate here, that...
The current TB treatment regimen involves a combination of drugs administered for an extended duration that could last 6 months to 2 years. This lead noncompliance and the emergence newer drug resistance strains.
Hepatitis E virus (HEV) is a major cause of hepatitis in normal and organ transplant individuals. HEV open reading frame-1 encodes polypeptide comprising the viral nonstructural proteins as well domains unknown function such macro domain (X-domain), V, DUF3729 Y. The are ubiquitously present from prokaryotes to human many positive-strand RNA viruses, playing important roles multiple cellular processes. Towards understanding domain, we characterized its interaction partners among other...
Abstract Neutralizing monoclonal antibodies are being found to be increasingly useful in viral infections. In hepatitis B infection, proven for passive prophylaxis. The preS1 region (21–47a.a.) of HBV contains the hepatocyte-binding domain crucial its attachment and infection hepatocytes. Antibodies against this neutralizing best suited immune-based neutralization HBV, especially view their not recognizing decoy particles. Anti-preS1 present serum spontaneously recovered individuals. We...
Non-small cell lung cancer (NSCLC), comprising 85% of cases, has been associated with resistance to chemo/radiotherapy. The hypoxic tumor micro-environment, where insufficient vasculature results in poor drug penetrance and sub-optimal chemotherapy the interiors contributes heavily this resistance. Additionally, epigenetic changes tumorigenic cells also change their response different forms therapy. In our study, we have investigated effectiveness a combination cisplatin scriptaid [a...
Hepatitis E virus (HEV) is associated with acute hepatitis disease, which may lead to chronic disease in immunocompromised individuals. The particularly severe among pregnant women (20–30% mortality). only licensed vaccine against HEV, available China, the E.coli purified recombinant virus-like particles (VLPs) encompassing 368-660 amino acids (aa) of viral ORF2 protein. capsid formed by protein, harbors three glycosylation sites. Baculo expression system has been employed generate a...
The emergence of new variants SARS-CoV-2 necessitates unremitting efforts to discover novel therapeutic monoclonal antibodies (mAbs). Here, we report an extremely potent mAb named P4A2 that can neutralize all the circulating concern (VOCs) with high efficiency, including highly transmissible Omicron. crystal structure Fab:RBD complex revealed residues RBD interact are a part ACE2-receptor-binding motif and not mutated in any VOCs. pan coronavirus pseudotyped neutralization assay confirmed is...
Phage display antibody (PDA) libraries, allows the rapid isolation and characterization of high specificity monoclonal antibodies for therapeutic diagnostic applications. However, selection positive binding clones from synthetic semi-synthetic libraries has an inherent bias towards containing randomly generated amber stop codons, complicating identification affinity antibodies. We screened Tomlinson I J library against receptor domain (RBD) SARS CoV2, eight which showed in phage ELISA,...