A5054989653- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Synthesis and Biological Evaluation
- SARS-CoV-2 and COVID-19 Research
- Synthesis and biological activity
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Parasitic Diseases Research and Treatment
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Parasites and Host Interactions
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Quinazolinone synthesis and applications
- Viral Infections and Outbreaks Research
- MXene and MAX Phase Materials
- Synthesis and Characterization of Heterocyclic Compounds
- Phytochemistry and Bioactivity Studies
- Ergonomics and Human Factors
- COVID-19 Clinical Research Studies
- Apelin-related biomedical research
- Paraoxonase enzyme and polymorphisms
Translational Health Science and Technology Institute
2020-2025
Barkatullah University
2024
Motilal Nehru National Institute of Technology
2024
Central Drug Research Institute
2010-2019
Academy of Scientific and Innovative Research
2016-2019
King George's Medical University
2017-2018
Council of Scientific and Industrial Research
2018
Pandit Ravishankar Shukla University
2015
Dr. Hari Singh Gour University
2015
University of Lucknow
2014
The high potential of quinazolinone containing natural products and their derivatives in medicinal chemistry led us to discover four novel series 53 compounds based on the concept molecular hybridization. Most synthesized analogues exhibited potent leishmanicidal activity against intracellular amastigotes (IC50 from 0.65 ± 0.2 7.76 2.1 μM) as compared miltefosine = 8.4 nontoxic toward J-774A.1 cell line Vero cells. Moreover, activation Th1 type suppression Th2 immune responses induction...
A series of pyrazolo(dihydro)pyridines was synthesized and evaluated for antileishmanial efficacy against experimental visceral leishmaniasis (VL). Among all compounds, 6d 6j exhibited better activity than miltefosine intracellular amastigotes. Compound (50 mg/kg/day) further studied Leishmania donovani/BALB/c mice via the intraperitoneal route 5 days displayed >91 >93% clearance splenic liver parasitic burden, respectively. Combination treatment with a subcurative dose (5 mg/kg) in BALB/c...
There is a desperate need for safe and effective vaccines, therapies, diagnostics SARS- coronavirus 2 (CoV-2), the development of which will be aided by discovery potent selective antibodies against relevant viral epitopes. Human phage display technology has revolutionized process identifying optimizing antibodies, providing facile entry points further applications. Herein, we use this to search targeting receptor-binding domain (RBD) CoV-2. Specifically, screened naïve human semisynthetic...
Abstract The SARS-CoV-2 virus has been rapidly evolving over the time and genetic variation led to generation of Variants Concerns (VoC), which have shown increased fitness. These VoC viruses contain key mutations in spike protein allowed better survival evasion host defense mechanisms. D614G mutation domain is found majority VoC; additionally, P681R/H at S1/S2 furin cleavage site junction also be highly conserved major VoCs; Alpha, Delta, Omicron, its’ current variants. impact these...
The objective of this study was to identify a nitroimidazo-oxazole lead molecule for the treatment visceral leishmaniasis (VL). A library 72 nitroimidazo-oxazoles evaluated in vitro their antileishmanial activity against luciferase-transfected DD8 amastigotes Leishmania donovani. On basis potency and pharmacokinetic properties, promising compounds were tested acute BALB/c mouse chronic hamster models VL via oral administration efficacy by microscopic counting after Giemsa staining. best...
Immuno-modulators in combination with antileishmanial drug miltefosine is a better therapeutic approach for treatment of Visceral Leishmaniasis (VL) as it not only reduces the dose but also shortens regimen. However, immunological mechanisms behind perceived benefits this therapy have been investigated detail. In present study, we hypothesized that potential use drugs target host addition to parasite might represent an alternative strategy therapy. We immune responses generated Leishmania...
Novel β-carboline–quinazolinone hybrids are able to inhibit <italic>Leishmania donovani</italic> TR (LdTR). Molecular docking studies investigate possible binding sites were performed.
Antileishmanial activities of a library synthetic chalcone analogues have been examined. Among them, five compounds (11, 14, 16, 17, 22, and 24) exhibited better activity than the marketed drug miltefosine in vitro studies against intracellular amastigotes form Leishmania donovani. Three promising compounds, were tested L. donovani/hamster model. Oral administration at concentration 100 mg/kg body weight per day for 5 consecutive days, resulted >84% parasite inhibition 7 post-treatment it...
IL-1R/TLR signaling plays a significant role in sensing harmful foreign pathogens and mounting effective innate adaptive immune responses. However, the precise mechanism by which Leishmania donovani, an obligate intramacrophagic pathogen, breaches host-protective immunity remains obscure. In this study, we report novel biphasic of Toll-interacting protein (Tollip), negative regulator pathway, disease progression experimental visceral leishmaniasis. We observed that during early hours...
The newly emerged novel coronavirus, SARS-CoV-2, the causative agent of COVID-19 has proven to be a threat human race globally, thus, vaccine development against SARS-CoV-2 is an unmet need driving mass vaccination efforts. receptor binding domain spike protein this coronavirus multiple neutralizing epitopes and associated with viral entry. Here we have designed characterized fragment 330-526 as (RBD ) two native glycosylation sites (N331 N343); potential subunit candidate. We initially RBD...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) initiates infection by attachment of the surface-exposed spike glycoprotein to host cell receptors. The (S) is a promising target for inducing immune responses and providing protection; thus ongoing efforts SARS-CoV-2 vaccine therapeutic developments are mostly spiraling around S glycoprotein. matured functional presented on virion surface as trimers, which contain two subunits, such S1 (virus attachment) S2 fusion). subunit...
Dengue virus infection in humans ranges from asymptomatic to severe infection, with ∼2.5 % overall disease fatality rate. Evidence of neurological manifestations is seen the form disease, which might be due direct invasion viruses into CNS system but poorly understood. In this study, we demonstrated that aged AG129 mice are highly susceptible dengue serotypes 1-4, and following adaptation, resulted generation neurovirulent strains showed enhanced replication, aggravated severity, increased...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a lethal beta-coronavirus that emerged in 2012. The virus part of the WHO blueprint priority list with concerning fatality rate 35%. Scientific efforts are ongoing for development vaccines, anti-viral and biotherapeutics, which majorly directed toward structural spike protein. However, effort challenging due to conformational instability protein evasion strategy posed by MERS-CoV. In this study, we have expressed purified MERS-CoV...
In view of the severe immunosuppression in visceral leishmaniasis (VL), a rational approach to effectively combat parasitic scourge would be enhance immune status host. Use CpG oligodeoxynucleotide (CpG-ODN) against has previously been reported, especially as an immunomodulator and adjuvant with various immunogens. present study, experiments were carried out BALB/c mice hamsters infected Leishmania donovani. Immunostimulating class B bacterial CpG-ODN namely, ODN-2006, was administered at...