A5022597901- SARS-CoV-2 and COVID-19 Research
- Autophagy in Disease and Therapy
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Endoplasmic Reticulum Stress and Disease
- Mosquito-borne diseases and control
- Mitochondrial Function and Pathology
- interferon and immune responses
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Computational Drug Discovery Methods
- Malaria Research and Control
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Mycobacterium research and diagnosis
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- RNA Research and Splicing
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Adipokines, Inflammation, and Metabolic Diseases
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Sirtuins and Resveratrol in Medicine
Institute of Life Sciences
2017-2024
University of California, San Diego
2009-2018
La Jolla Alcohol Research
2015
Office of Infectious Diseases
2014-2015
University of Hyderabad
2007-2012
Significance Persistent hepatitis C virus (HCV) infection is associated with mitochondrial liver injury. Mitochondrial quality control established as a physiological adaptation to This study provides new insight into how HCV disrupts dynamics and evades apoptosis innate immunity sustain persistent viral infection. promoted dynamin-related protein 1-mediated fission, followed by mitophagy. Interference of HCV-induced fission mitophagy led the suppression secretion, decrease in glycolysis ATP...
Hepatitis C Virus (HCV) induces intracellular events that trigger mitochondrial dysfunction and promote host metabolic alterations. Here, we investigated selective autophagic degradation of mitochondria (mitophagy) in HCV-infected cells. HCV infection stimulated Parkin PINK1 gene expression, induced perinuclear clustering mitochondria, promoted translocation Parkin, an initial event mitophagy. Liver tissues from chronic patients also exhibited notable levels induction. Using multiple...
Article24 August 2018Open Access Source DataTransparent process TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis autophagy Kautilya Kumar Jena Cell Biology Infectious Diseases Unit, Institute Life Sciences, Bhubaneswar, India School Biotechnology, KIIT University, Search for more papers this author Srinivasa Prasad Kolapalli Subhash Mehto orcid.org/0000-0002-5351-7850 Parej Nath Biswajit Das Tumor Microenvironment Animal Models, Manipal Manipal,...
Lipids play a crucial role in multiple aspects of hepatitis C virus (HCV) life cycle. HCV modulates host lipid metabolism to enrich the intracellular milieu with lipids facilitate its proliferation. However, very little is known about influence on uptake from bloodstream. Low-density lipoprotein receptor (LDLR) involved cholesterol rich low-density (LDL) particles The association lipoproteins implicates their entry; however, precise LDLR entry still remains controversial. Here, we...
Hepatitis B virus (HBV) suppresses innate immune signaling to establish persistent infection. Although HBV is a DNA virus, its pre-genomic RNA (pgRNA) can be sensed by RIG-I and activates MAVS mediate interferon (IFN) λ synthesis. Despite of the activation RIG-I-MAVS axis pgRNA, underlying mechanism explaining how infection fails induce interferon-αβ synthesis remained uncharacterized. We demonstrate that induced parkin able recruit linear ubiquitin assembly complex (LUBAC) mitochondria...
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, has emerged as a global pandemic worldwide. In this study, we used ARTIC primers–based amplicon sequencing to profile 225 SARS-CoV-2 genomes from India. Phylogenetic analysis of 202 high-quality assemblies identified presence all five reported clades 19A, 19B, 20A, 20B, and 20C in population. The analyses revealed Europe Southeast Asia two major routes for introduction India...
The SARS-CoV2 is a highly contagious pathogen that causes COVID-19 disease. It has affected millions of people globally with an average lethality ~3%. There urgent need drugs for the treatment COVID-19.In current studies, we have used bioinformatics techniques to screen FDA approved against nine proteins identify repurposing. Additionally, analyzed if identified molecules can also affect human whose expression in lung changed during infection. Targeting such genes may be beneficial strategy...
Abstract Hepatitis C virus (HCV) relies on host lipid metabolic pathways for its replication, assembly, secretion, and entry. HCV induces de novo lipogenesis, inhibits β-oxidation, lipoprotein export resulting in a lipid-enriched cellular environment critical proliferation. We investigated the effects of hypolipidemic agent, nordihydroguaiaretic acid (NDGA), lipid/fatty synthesis life cycle. NDGA negated HCV-induced alteration homeostasis. decreased sterol regulatory element binding protein...
ABSTRACT Hepatitis C virus (HCV) exists as a lipoprotein-virus hybrid lipoviroparticle (LVP). In vitro studies have demonstrated the importance of apolipoproteins in HCV secretion and infectivity, leading to notion that coopts very-low-density lipoprotein (VLDL) for its egress. However, mechanisms involved particle assembly egress are still elusive. The biogenesis VLDL particles occurs endoplasmic reticulum (ER), followed by subsequent lipidation ER Golgi compartment. mature through...
Syrian golden hamsters (Mesocricetus auratus) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manifests lung pathology. In this study, efforts were made to check the infectivity of a local SARS-CoV-2 isolate in self-limiting and non-lethal hamster model evaluate differential expression proteins during infection convalescence. The findings study confirm vivo. Analysis clinical parameters tissue samples show pathophysiological manifestation similar that reported...
Many viruses target host cell mitochondria to create a microenvironment conducive viral dissemination. Dengue virus also exploits facilitate its life cycle.
The nonstructural protein 4A (NS4A) of flaviviruses has been implicated as a "central organizer" the membrane-bound replication complex during virus replication. However, its role in host responses to infection is not understood. Using yeast-two-hybrid library screen, we identified multitude proteins interacting with Japanese encephalitis (JEV) NS4A protein. Several these are known localize mitochondria. One was PTEN-induced kinase 1 (PINK1), serine/threonine-protein for mitophagy. Here,...
All eukaryotic cells utilize autophagy for protein and organelle turnover, thus assuring subcellular quality control, homeostasis, survival. In order to address recent advances in identification of human associated genes, describe on a system-wide level, we established an autophagy-centered gene interaction network by merging various primary data sets retrieving respective data. The resulting ('AXAN') was analyzed with respect subnetworks, e.g. the prime subnetwork (including core machinery,...
Dengue virus infection in humans ranges from asymptomatic to severe infection, with ∼2.5 % overall disease fatality rate. Evidence of neurological manifestations is seen the form disease, which might be due direct invasion viruses into CNS system but poorly understood. In this study, we demonstrated that aged AG129 mice are highly susceptible dengue serotypes 1-4, and following adaptation, resulted generation neurovirulent strains showed enhanced replication, aggravated severity, increased...
In this study, we aimed to evaluate the therapeutic potential of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an activator AMP-activated protein kinase, for ameliorating high-fat diet (HFD)-induced pathophysiology in mice. We also determine whether beneficial effects AICAR were dependent on adiponectin. Furthermore, human adipose tissue was used examine effect ex vivo. Six-week-old male C57BL/6J wild-type and Adipoq −/− mice fed a standard-fat (10% fat) or HFD (60% 12 weeks given...