A5101399423- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Renal and related cancers
- Cancer-related gene regulation
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Livestock Management and Performance Improvement
- Genetic Syndromes and Imprinting
- Trigeminal Neuralgia and Treatments
- Genetic and rare skin diseases.
- Bioenergy crop production and management
- Milk Quality and Mastitis in Dairy Cows
- Cutaneous lymphoproliferative disorders research
- Effects of Environmental Stressors on Livestock
- Indigenous Peoples' Rights and Law
National Dairy Research Institute
2015-2024
Indian Council of Agricultural Research
2020
All India Institute of Medical Sciences
2018
Hand-made cloning (HMC) has proved to be an efficient alternative the conventional micromanipulator-based technique in some domestic animal species. This study reports development of effective culture system for vitro zona-free cloned buffalo (Bubalus bubalis) embryos reconstructed using adult skin fibroblast cells as nucleus donor. Cleavage and blastocyst rates observed were 52 0% modified Charles Rosenkrans 2 (mCR2), 61 4.6% Synthetic Oviductal Fluid (mSOF), 82 40.3% Research Vitro Cleave...
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm animal species for improved production health traits; however, these tools are rarely in the buffalo can play a pivotal role milk meat tropical subtropical countries. In this study, we aimed myostatin (
The present study evaluated the effects of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth (FGF) 2 and epidermal (EGF) on proliferation expression some genes in spermatogonial cells. Spermatogonial cells were isolated from prepubertal buffalo testes enriched by double enzyme treatment, filtration through 80- 60-μm nylon mesh filters, differential plating lectin-coated dishes Percoll density gradient centrifugation. Cells then cultured a Sertoli feeder layer formed...
Cumulus cells provide cellular interactions and growth factors required for oogenesis. In vitro studies of oogenesis are limited primarily because the paucity their source, first trimester fetal gonads, small number germ lineage precursor present within these tissues. order to understand this obscure but vitally important process, study was designed direct differentiation embryonic stem (ES) into cells. For purpose, buffalo ES were differentiated, as embryoid bodies (EBs) monolayer adherent...
The 490 and 380 performance records of Karan Fries Swiss cows developed through crossbreeding followed by inter-se mating were evaluated for production, reproduction disposal rate. Duration study (1982-92) was grouped into five periods (1982-83; 1984-85; 1986-87; 1988-89; 1990-92) each year divided four seasons (Winter: Dec.- Jan.; Spring: Feb.-March; Summer: April-June; Rainy: July-Sept.; Autumn: Oct.-Nov). Data also classified according to sire level inbreeding. least squares means FLY...
In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth (FGF) 2, and epidermal (EGF) on expression some self-renewal-related microRNAs (miRs) in putative buffalo spermatogonial stem cells (SSCs). The SSCs were cultured a Sertoli feeder layer, colony formation was observed between 7 10 days. SSC colonies expressed markers specific for undifferentiated type A spermatogonia pluripotency markers. After 15 days initial culture,...
The aim of the present study was to compare transgenic cells, containing human insulin gene kept under control mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic for examining potential production embryos following somatic cell nuclear transfer (SCNT). construct delivered into fetal fibroblasts (BFF) by nucleofection which, transfected cells were selected culture in presence G418 3 weeks. Transgene integration BFF genome confirmed...
In the present study, oocytes retrieved from cross bred Karan Fries cows by ovum pick-up technique were graded into Group 1 and 2, based on morphological appearance of individual cumulus–oocyte complexes (COCs). To analyze whether developmental potential COCs bears a relation to appearance, relative expression panel genes associated with; (a) interaction (Cx43, Cx37, GDF9 BMP15), (b) fertilization (ZP2 ZP3), (c) embryonic development (HSF1, ZAR1 bFGF) (d) apoptosis survival (BAX, BID BCL-XL,...
Spermatogonial stem cells (SSCs) self-renew and produce a large number of differentiated germ to maintain normal spermatogenesis. However, the growth factors crucial for SSC self-renewal mechanism underlying this process remain unclear. In present study, serum-free culture media was used evaluate effect several on expression some markers related genes. The putative SSCs were cultured buffalo Sertoli cell feeder layer in KO-DMEM +10% KOSR. colony formation observed between 7 10 days. colonies...
Despite the success of cloning technology in production offspring across several species, its application on a wide scale is severely limited by very low rate obtained with cloned embryos. The expression profile microRNAs (miRNAs) embryos throughout embryonic development reported to deviate from regular patterns. present study aimed at determining dynamics global miRNA and in-vitro fertilization (IVF) pre-implantation different developmental stages, i.e., two-cell, eight-cell, blastocyst...
This study was aimed at establishing buffalo embryonic stem cells (ESCs) from in vitro fertilized (IVF), parthenogenetic, and hand-made cloned (HMC) embryos to check their equivalency terms of cell marker expression, longevity, proliferation, differentiation pattern. ESCs derived all three sources were found by immunofluorescence express the pluripotency markers SSEA-4, TRA-1-60, TRA-1-81, OCT4, SOX2 able form embryoid bodies containing expressing genes specific endoderm (AFP, HNF4, GATA4),...
The aim of this study was to investigate the transcriptional profile and role WNT3A signalling in maintaining buffalo embryonic stem (ES) cells a pluripotent state induction their differentiation. ES were derived from embryos produced by vitro fertilisation (iESC), parthenogenesis (pESC) hand-made cloning (cESC). expression WNT3A, its receptors intermediate pathways found be conserved three different sources. expressed but not embryoid bodies iESC or fetal fibroblast cells. It revealed...
Epigenetic reprogramming is an indispensable process during the course of mammalian development, but aberrant in cloned embryos. The aim this study was to examine effect donor cell treatment with histone deacetylase (HDAC) inhibitor m-carboxycinnamic acid bishydroxymide (CBHA) on embryo development and establish its optimal concentration. Different concentrations CBHA (2.5, 5.0, 10.0, 20.0 μM) were used treat buffalo adult fibroblast cells for 24 hours proliferation, gene expression,...
Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although rate blastocyst formation (43.8% ± 1.31% vs. 42.2% 1.22%) was similar, total cell number (333 10.4 270 10.9) higher (p < 0.05) whereas apoptotic index (6.39 0.25 8.52 0.38) lower than blastocysts. In blastocysts, global level H3K18ac found to be in following order: male>female>IVF (in vitro fertilization) blastocysts...