A5031909097- Cellular transport and secretion
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Fluorine in Organic Chemistry
- Cyclopropane Reaction Mechanisms
- Enzyme Structure and Function
- Synthesis and Catalytic Reactions
- Lipid Membrane Structure and Behavior
- Catalytic C–H Functionalization Methods
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Synthesis and Biological Evaluation
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Pancreatic function and diabetes
- Click Chemistry and Applications
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Metal-Catalyzed Oxygenation Mechanisms
- Traditional Chinese Medicine Studies
- Phytochemistry and Bioactivity Studies
- Crystallography and molecular interactions
- Drug Transport and Resistance Mechanisms
- Surgical Sutures and Adhesives
Second Affiliated Hospital of Chengdu University of Traditional Chinese
2025
Chengdu University
2025
Guangxi Medical University
2024
University of Chinese Academy of Sciences
2024
Kunming Institute of Botany
2024
Chinese Academy of Sciences
2024
Xiamen University
2023
St James's University Hospital
2021
University of Leeds
2021
University of Edinburgh
2021
Posttranslational lipidation provides critical modulation of the functions some proteins. Isoprenoids (i.e., farnesyl or geranylgeranyl groups) are attached to cysteine residues in proteins containing C-terminal CAAX sequence motifs (where A is an aliphatic residue and X any residue). Isoprenylation followed by cleavage AAX amino acid and, cases, additional proteolytic cuts. We determined crystal structure protease Ste24p, a zinc metalloprotease catalyzing two steps maturation yeast mating...
Neuronal electrical impulse propagation is facilitated by the myelin sheath, a compact membrane surrounding axon. The sheath highly enriched in galactosylceramide (GalCer) and its sulfated derivative sulfatide. Over 50% of GalCer sulfatide hydroxylated integral enzyme fatty acid 2-hydroxylase (FA2H). hydroxylation contributes to nature membrane, mutations FA2H result debilitating leukodystrophies spastic paraparesis. We report here 2.6 Å crystal structure sphingolipid α-hydroxylase (Scs7p),...
Memapsin 2 (β-secretase) is a membrane-associated aspartic protease that initiates the hydrolysis of β-amyloid precursor protein (APP) leading to production amyloid-β and onset Alzheimer's disease (AD). Both memapsin APP are transported from cell surface endosomes where takes place. Thus, intracellular transport mechanism important for understanding pathogenesis AD. We have previously shown cytosolic domain contains an acid-cluster-dileucine (ACDL) motif binds VHS GGA proteins (He et al....
Eukaryotic lanthionine synthetase C-like protein 1 (LanCL1) is homologous to prokaryotic cyclases, yet its biochemical functions remain elusive. We report the crystal structures of human LanCL1, both free and complexed with glutathione, revealing glutathione binding a zinc ion at putative active site formed by conserved GxxG motifs. also demonstrate in vitro affinity analysis that LanCL1 binds specifically SH3 domain signaling protein, Eps8. Importantly, expression mutants defective Eps8...
A copper(i)-mediated carboamination cascade reaction between vinyl azides and aryldiazonium salts is described. Functionally diverse N2-substituted 1,2,3-triazoles were obtained in moderate to good yields through novel difunctionalization of by salt sources. This method has a wide scope, functional-group tolerance insensitivity an ambient atmosphere.
Dioxygenases catalyze dioxygen incorporation into various organic compounds and play a key role in the complex degradation pathway of mono- polycyclic aromatic hetero-aromatic compounds. Here we report crystal structure gentisate 1,2-dioxygenase from Silicibacter pomeroyi (GDOsp) at 2.8 A resolution. The enzyme possessed conserved three-dimensional bicupin family, forming homotetramerization. However, each subunit GDOsp unusually contained two ferrous centers that were located its homologous...
Rabex-5 is a guanine nucleotide exchange factor (GEF) for Rab5. Here, we report the identification of novel functional domain that essential its membrane targeting and Rab5 GEF activity in vivo. The data show full-length efficiently activates cell. However, itself (residues 135-399) inactive this respect, despite vitro. Generation characterization series constructs reveal unable to target early endosomes sequence N-terminal can restore endosomal ability activate This region 81-135) termed...
Cancer cells generally exhibit 'iron addiction' phenotypes, which contribute to their vulnerability ferroptosis inducers. Ferroptosis is a newly discovered form of programmed cell death caused by iron-dependent lipid peroxidation. In the present study, pacidusin B, dichapetalin-type triterpenoid from Phyllanthus acidus (L.) Skeels (Euphorbiaceae), induces in HT1080 human fibrosarcoma line. Cells treated with B exhibited morphological characteristic 'ballooning' phenotype ferroptosis. The...
Abstract Spliceosomal small nuclear RNAs (snRNAs) are a fundamental class of non‐coding abundant in the nucleoplasm eukaryotic cells, playing crucial role splicing precursor messenger (pre‐mRNAs). They transcribed by DNA‐dependent RNA polymerase II (Pol II) or III III), and undergo subsequent processing 3′ end cleavage to become mature snRNAs. Numerous protein factors involved transcription initiation, elongation, termination, splicing, cellular localization, terminal modification processes...
GTPase domain crystal structures of Rab5a wild type and five variants with mutations in the phosphate-binding loop are reported here at resolutions up to 1.5 Å. Of particular interest, A30P mutant was crystallized complexes GDP, GDP+AlF<sub>3</sub>, authentic GTP, respectively. The other variant crystals were obtained a non-hydrolyzable GTP analog, GppNHp. All solved same form, providing an unusual opportunity compare small GTPases different catalytic rates. exhibits dramatically reduced...
A novel short-chain (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) from Candida parapsilosis exhibits coenzyme specificity for NADPH over NADH. It catalyzes an anti-Prelog type reaction to reduce 2-hydroxyacetophenone into (S)-1-phenyl-1,2-ethanediol. The coding gene was overexpressed in Escherichia coli and the purified protein crystallized. crystal structure of apo-form solved 2.7 resolution. This forms a homo-tetramer with broken 2-2-2 symmetry. overall fold each SCR subunit is similar...
The endonuclease Artemis is responsible for opening DNA hairpins during V(D)J recombination and processing a subset of pathological double-strand breaks. an attractive target the development therapeutics to manage various B cell T tumors, because failure open accumulation chromosomal breaks may reduce proliferation viability pre-T pre-B derivatives. However, structure-based drug discovery specific inhibitors has been hampered by lack crystal structures. Here, we report structure catalytic...
α-Dioxygenases (α-DOX) oxygenate fatty acids into 2(R)-hydroperoxides. Despite the low level of sequence identity, α-DOX share common catalytic features with cyclooxygenases (COX), including use a tyrosyl radical during catalysis. We determined X-ray crystal structure Arabidopsis thaliana to 1.5 Å resolution. The is monomeric, predominantly α-helical, and comprised two domains. base domain exhibits degree structural homology membrane-binding COX but lies in similar position respect domain....
A number of proteins involved in intracellular signaling contain regions homology to the product src oncogene that are termed Src-homology (SH) 2 domains. SH2 domains believed mediate association these with various tyrosine-phosphorylated receptors a growth factor-dependent manner. We have examined kinetic characteristics one interactions, binding phospholipase C gamma 1 receptor for epidermal factor (EGF). Bacterial fusion were prepared containing two PLC and labeled metabolically...
Heat shock response (HSR) is a ubiquitous cellular mechanism that copes with variety of stresses. This mediated by family transcriptional activators, heat factors (HSFs), which are under tight regulation. HSF binding protein 1 (HSBP1) negative regulator HSR and reported to bind specifically the active trimeric form HSF1, thus inhibiting its activity. HSBP1 contains heptad-repeats in primary sequence was believed stay trimer solution. We report crystal structure trimerization domain M30I/L55P...
The molecular architecture and composition of the outer membrane (OM) Treponema pallidum (Tp), noncultivable agent venereal syphilis, differ considerably from those typical Gram-negative bacteria. Several years ago we described TP0453, only lipoprotein associated with inner leaflet Tp OM. Whereas polypeptides other treponemal lipoproteins are hydrophilic, non-lipidated TP0453 can integrate into membranes, a property attributed to its multiple amphipathic helices (AHs). Furthermore,...
A novel and efficient aldol reaction of readily available vinyl azides with trifluoromethyl ketones by copper catalysis is developed. The proposed to go through a nucleophilic trapping as source, leading the assembly diverse trifluoromethylated compounds under mild conditions in satisfactory yield.
GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other carrying out The GAT domain has been identified as the structural entity binds membrane-bound ARF, molecular switch regulating vesicle−coat assembly. It also directly interacts rabaptin5, an essential component endosome fusion. 2.8 Å resolution crystal structure human GGA1 is reported here. helices and elongated...