A5076715041- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Lung Cancer Treatments and Mutations
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Viral-associated cancers and disorders
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Multiple Myeloma Research and Treatments
- Hematopoietic Stem Cell Transplantation
- Advanced Biosensing Techniques and Applications
- Mycobacterium research and diagnosis
- Effects and risks of endocrine disrupting chemicals
- Genetic factors in colorectal cancer
Affiliated Hospital of Nantong University
2025
Second Affiliated Hospital of Nantong University
2025
Nantong University
2025
Kingmed Diagnostics
2020-2024
Southwest Hospital
2014-2024
Army Medical University
2014-2024
East China Normal University
2012-2024
Affiliated Hospital of Hebei University
2019-2024
Hebei North University
2024
City Of Hope National Medical Center
2015-2023
Lymphomas arising from NK or γδ-T cells are very aggressive diseases and little is known regarding their pathogenesis. Here we report frequent activating mutations of STAT3 STAT5B in NK/T-cell lymphomas (n=51), γδ-T-cell (n=43) cell lines (n=9) through next generation and/or Sanger sequencing. N642H particularly all forms lymphomas. associated with increased phosphorylated protein a growth advantage to transduced normal cells. Growth-promoting activity the mutants can be partially inhibited...
Significance A tumor comprising many cells can be compared to a natural population with individuals. The amount of genetic diversity reflects how it has evolved and influence its future evolution. We evaluated single by sequencing or genotyping nearly 300 regions from the tumor. When data were analyzed modern theory, we estimated more than 100 million coding region mutations in this unexceptional extreme implies evolution under non-Darwinian mode. In contrast, prevailing view Darwinian...
We present the analysis of evolution tumors in a case hepatocellular carcinoma. This is particularly informative about cancer growth dynamics and underlying driving mutations. sampled nine different sections from three seven more adjacent nontumor tissues. Selected were subjected to exon as well whole-genome sequencing. Putative somatic mutations then individually validated across all 9 tumor 7 sections. Among validated, 24 amino acid changes; addition, 22 large indels/copy number variants...
We report an aptamer discovery technology that reproducibly yields higher affinity aptamers in fewer rounds compared to conventional selection. Our method (termed particle display) transforms libraries of solution-phase into "aptamer particles", each displaying many copies a single sequence on its surface. then use fluorescence-activated cell sorting (FACS) individually measure the relative affinities >10(8) particles and sort them high-throughput manner. Through mathematical analysis, we...
To identify tumor suppressor genes epigenetically silenced by promoter hypermethylation in extranodal natural killer cell lymphoma (NKCL).Promoter methylation was analyzed with global and locus-specific assays NKCL cases NK lines. Gene expression profiles were used to for which aberrant associated transcriptional silencing. Selected DNA methylations validated RRBS, pyrosequencing, or q-MSP. Decitabine treatment performed evaluate reactivation of methylated genes. The effect evaluated...
Currently, there is no effective treatment for chronic skin radiation injury, which burdens patients significantly. Previous studies have shown that cold atmospheric plasma has an apparent therapeutic effect on acute and injuries in clinical. However, whether CAP radiation-induced injury not been reported. We created 35Gy X-ray exposure within 3 * cm2 region of the left leg rats applied to wound bed. Wound healing, cell proliferation apoptosis were examined vivo or vitro. alleviated by...
Abstract Aptamers are a promising class of affinity reagents because they chemically synthesized, thus making them highly reproducible and distributable as sequence information rather than physical entity. Although many high‐quality aptamers have been previously reported, it is difficult to routinely generate that possess both high specificity. One the reasons conventional aptamer selection can only be performed either for (positive selection) or specificity (negative selection), but not...
Due to the acquired drug resistance, potency of cisplatin-based chemotherapy is limited in lung cancer, which a big obstacle clinical treatment cancer. Abundant evidence has revealed that circular RNAs (circRNAs) exerted facilitating or suppressive function on tumorigenesis multiple cancers. The oncogenic role circ-ABCB10 breast cancer and clear cell renal carcinoma been validated recent researches. However, regulatory mechanism its relation cellular sensitivity cisplatin poorly...
The ABC/2 method is usually applied to evaluate intracerebral hemorrhage (ICH) volume on computed tomography (CT), although it might be inaccurate and not applicable in estimating extradural or subdural (EDH, SDH) due their irregular hematoma shapes. This study aimed deep framework optimized for the segmentation quantification of ICH, EDH, SDH.The training datasets were 3,000 images retrospectively collected from a collaborating hospital (Hospital A) segmented by Dense U-Net framework. Three...
It's challenging work to identify disease-causing genes from the next-generation sequencing (NGS) data of patients with Mendelian disorders. To improve this situation, researchers have developed many phenotype-driven gene prioritization methods using a patient's genotype and phenotype information, or information only as input rank candidate's pathogenic genes. Evaluations these ranking provide practitioners convenience for choosing an appropriate tool their workflows, but retrospective...
Nucleic acid-based aptamers possess many useful features that make them a promising alternative to antibodies and other affinity reagents, including well-established chemical synthesis, reversible folding, thermal stability low cost. However, the selection process typically used generate (SELEX) often requires significant resources can fail yield with sufficient specificity. A number of seminal theoretical models numerical simulations have been reported in literature offering insights into...
Affinity reagents that bind to specific molecular targets are an essential tool for both diagnostics and targeted therapeutics. There is a particular need advanced technologies the generation of specifically target cell-surface markers, because transmembrane proteins notoriously difficult express in recombinant form. We have previously shown microfluidics offers many advantages generating affinity against purified protein targets, we now significantly extended this approach achieve...
Many analytical techniques benefit greatly from the use of affinity reagent pairs, wherein each recognizes a discrete binding site on target. For example, antibody pairs have been widely used to dramatically increase specificity enzyme linked immunosorbent assays (ELISA). Nucleic acid-based aptamers offer many advantageous features relative protein-based reagents, including well-established chemical synthesis, thermostability, and low production cost. However, generation suitable aptamer has...
Abstract Purpose: We performed detailed genomic analysis on 87 cases of de novo diffuse large B-cell lymphoma germinal center type (GCB DLBCL) to identify characteristics that are associated with survival in those treated R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone). Experimental Design: The were extensively characterized by combining the results IHC, cell-of-origin gene expression profiling (GEP; NanoString), double-hit GEP (DLBCL90), FISH cytogenetic for...
Natural killer/T-cell lymphoma is a rare but aggressive neoplasm with poor prognosis. Despite previous reports that showed potential tumor suppressors, such as PRDM1 or oncogenes associated the etiology of this malignancy, role long non-coding RNAs in natural pathobiology has not been addressed to date. Here, we aim identify cancer-associated dysregulated and signaling pathways biological processes these cases transcriptionally regulated by PRDM1. RNA-Seq analysis revealed 166 66 be...
Video-assisted thoracic surgery (VATS) generally involves endotracheal intubation under general anesthesia. However, inevitably, this may cause intubation-related complications and prolong the postoperative recovery process. Gradually, non-intubated video-assisted (NIVATS) is increasingly being utilized. its safety efficacy remain controversial.Randomized controlled trials (RCTs) published up to August 2020 were selected from Cochrane Library, Web of Science, PubMed, Embase,...