A5031023967- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Biosensors and Analytical Detection
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Single-cell and spatial transcriptomics
- Retinoids in leukemia and cellular processes
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Phagocytosis and Immune Regulation
- Respiratory viral infections research
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
Vanderbilt University Medical Center
2023-2024
Aptitude Medical Systems
2016-2022
Abstract Aptamers are a promising class of affinity reagents because they chemically synthesized, thus making them highly reproducible and distributable as sequence information rather than physical entity. Although many high‐quality aptamers have been previously reported, it is difficult to routinely generate that possess both high specificity. One the reasons conventional aptamer selection can only be performed either for (positive selection) or specificity (negative selection), but not...
ABSTRACT Throughout life, humans experience repeated exposure to viral antigens through infection and vaccination, resulting in the generation of diverse, antigen-specific antibody repertoires. A paramount feature antibodies that enables their critical contributions counteracting recurrent novel pathogens, consequently fostering utility as valuable targets for therapeutic vaccine development, is exquisite specificity displayed against target antigens. Yet, there still limited understanding...
Broadly reactive antibodies that target sequence-diverse antigens are of interest for vaccine design and monoclonal antibody therapeutic development because they can protect against multiple strains a virus provide barrier to evolution escape mutants. Using LIBRA-seq (linking B cell receptor antigen specificity through sequencing) data the repertoire an individual chronically infected with human immunodeficiency type 1 (HIV-1), we identified lineage IgG3 predicted bind HIV-1 Envelope (Env)...
SUMMARY Throughout life, humans experience repeated exposure to viral antigens through infection and vaccination, building diverse antigen-specific antibody repertoires. In recent years, these repertoires have become an important source for novel antibody-based antiviral therapeutics, yet there is still limited understanding of the determinants antibody-antigen specificity. Here, we generated a large dataset mapping sequence antigen specificity thousands B cells, by screening set healthy...
Abstract Aptamers are a promising class of affinity reagents because they chemically synthesized, thus making them highly reproducible and distributable as sequence information rather than physical entity. Although many high‐quality aptamers have been previously reported, it is difficult to routinely generate that possess both high specificity. One the reasons conventional aptamer selection can only be performed either for (positive selection) or specificity (negative selection), but not...
Global deployment of vaccines poses significant challenges in the distribution and use accompanying immunoassays, one standard methods for quality control vaccines, particularly when establishing assays countries worldwide to support testing/release upon importation. This work describes our effort toward developing an integrated, portable device carry out affinity viral particles quantification by incorporating (i) aptamers, (ii) microfluidic devices, (iii) electrochemical detection. We...
ABSTRACT Human respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are frequent drivers of morbidity mortality in susceptible populations, most often infantile, older adults, immunocompromised. The primary target neutralizing antibodies is the fusion (F) glycoprotein on surface RSV hMPV virion. As a result structural conservation between F, three antigenic regions known to induce cross-neutralizing responses: sites III, IV, V. Leveraging LIBRA-seq, we identify five RSV/hMPV...
LIBRA-seq (linking B cell receptor to antigen specificity by sequencing) provides a powerful tool for interrogating the antigen-specific compartment and identifying antibodies against targets of interest. Identification noise in single-cell sequencing data, such as LIBRA-seq, is critical improving binding predictions downstream applications including antibody discovery machine learning technologies.
Abstract The traditional process of antibody discovery is limited by inefficiency, high costs, and low success rates. Recent approaches employing artificial intelligence (AI) have been developed to optimize existing antibodies generate sequences in a target-agnostic manner. In this work, we present MAGE (Monoclonal Antibody GEnerator), sequence-based Protein Language Model (PLM) fine-tuned for the task generating paired human variable heavy light chain against targets interest. We show that...
ABSTRACT Consistent elicitation of serum antibody responses that neutralize diverse clades HIV-1 remains a primary goal vaccine research. Prior work has defined key features soluble Envelope (Env) immunogen cocktails influence the neutralization breadth and potency multivalent vaccine-elicited including number Env strains in regimen. We designed immunization groups consisted different numbers to be used cocktail strategy: smallest (group 2) set two strains, which were subset three made up...
LIBRA-seq (linking B cell receptor to antigen specificity by sequencing) provides a powerful tool for interrogating the antigen-specific compartment and identifying antibodies against targets of interest. Identification noise in count data is critical improving binding predictions downstream applications including antibody discovery machine learning technologies.
Consistent elicitation of serum antibody responses that neutralize diverse clades HIV-1 remains a primary goal vaccine research. Prior work has defined key features soluble Envelope (Env) immunogen cocktails influence the neutralization breadth and potency multivalent vaccine-elicited including number Env strains in regimen. We designed immunization groups consisted different numbers SOSIP to be used cocktail strategy: smallest (group 2) set two strains, which were subset three made up group...