A5002202936- Estrogen and related hormone effects
- Phytochemistry and Biological Activities
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Natural product bioactivities and synthesis
- Genomics, phytochemicals, and oxidative stress
- Cancer Risks and Factors
- Traditional Chinese Medicine Analysis
- Essential Oils and Antimicrobial Activity
- Vibrio bacteria research studies
- DNA Repair Mechanisms
- Bioactive Compounds and Antitumor Agents
- Synthesis and Biological Evaluation
- Glutathione Transferases and Polymorphisms
- Bioactive Natural Diterpenoids Research
- Biological Activity of Diterpenoids and Biflavonoids
- Plant biochemistry and biosynthesis
- Aquaculture disease management and microbiota
- Sesquiterpenes and Asteraceae Studies
- Ubiquitin and proteasome pathways
- Antibiotic Resistance in Bacteria
- Cancer therapeutics and mechanisms
- Phytochemical Studies and Bioactivities
- Bacteriophages and microbial interactions
- Seed and Plant Biochemistry
University of Nebraska Medical Center
2015-2025
Zhejiang University
2001-2025
Nebraska Medical Center
2011-2024
Italian Institute of Technology
2024
University of Engineering and Technology Lahore
2024
University of the Punjab
1999-2022
University of North Carolina at Chapel Hill
2018-2022
National Taiwan University Hospital
2022
Osaka Prefecture University
2014-2022
Superior University
2021
In this manuscript, for the first time, we report synthesis of a PROTAC that selectively degrades cyclin dependent kinase 9 (CDK9).
Fuchs endothelial corneal dystrophy (FECD) is a leading cause of (CE) degeneration resulting in impaired visual acuity. It genetically complex and age-related disorder, with higher incidence females. In this study, we established nongenetic FECD animal model based on the physiologic outcome CE susceptibility to oxidative stress by demonstrating that exposure ultraviolet A (UVA) recapitulates morphological molecular changes FECD. Targeted irradiation mouse corneas UVA induced reactive oxygen...
Strong evidence supports the idea that specific metabolites of estrogens, mainly catechol estrogen-3,4-quinones, can react with DNA to become endogenous initiators breast, prostate, and other human cancers. Oxidation estrogen 4-hydroxyestradiol (4-OHE2) 2-OHE2 leads quinones, estradiol-3,4-quinone (E2-3,4-Q) estradiol-2,3-quinone (E2-2,3-Q), respectively. The reaction E2-3,4-Q affords predominantly depurinating adducts 4-OHE2-1-N3Ade 4-OHE2-1-N7Gua, whereas E2-2,3-Q yields newly synthesized...
Abstract Exposure to estrogens is a risk factor for breast cancer. Specific estrogen metabolites may initiate cancer and other cancers. Genotoxicity be caused by cytochrome P450 (CYP)–mediated oxidation of catechol quinones that react with DNA form depurinating estrogen-DNA adducts. CYP1B1 favors quinone formation catalyzing 4-hydroxylation, whereas NAD(P)H oxidoreductase 1 (NQO1) catalyzes the protective reduction catechols. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces expression...
We assessed the relationship between chronic arsenic exposure through drinking water with respiratory complications and humoral immune response by measuring serum immunoglobulin profiles in affected subjects (arsenicosis patients) living endemic rural villages of Bangladesh. The duration was determined detailed history patients (n = 125) levels urine samples were determined. mean 7.4 ± 5.3 y, 216 211 223 302 μ g/L, respectively, compared to 11 20 29 19 unexposed subjects. There high...
The El Tor biotype of Vibrio cholerae O1, causing the current seventh pandemic cholera, has replaced classical biotype, which caused sixth pandemic. CTX prophages encoding cholera toxin in two biotypes have distinct repressor ( rstR ) genes. Recently, new variants strains that carry type (CTX class prophage emerged. These “hybrid” apparently originate through lateral gene transfer and recombination events. To explore possible donors its mode transfer, we tested environmental V. isolates for...
Abstract Using a single nucleotide polymorphism association study in 52 men with prostate cancer receiving docetaxel, we found that individuals carrying two copies of the CYP1B1*3 polymorphic variant had poor prognosis after docetaxel-based therapies compared at least one copy CYP1B1*1 allele (30.6 versus 12.8 months; P = 0.0004). The between and response to therapy was not observed similar subjects non-taxane-based (P 0.18). systemic clearance docetaxel also unrelated CYP1B1 genotype status...
ABSTRACT Seasonal epidemics of cholera in Bangladesh are self-limited nature, presumably due to phage predation the causative Vibrio cholerae during late stage an epidemic, when patients excrete large quantities their stools. To further understand mechanisms involved, we studied effect on infectivity and survival V. shed The 50% infectious dose stool vibrios infant mice was ∼10-fold higher stools contained a (1.8 × 10 3 5.7 6 PFU/ml) than did not contain detectable phage. In competition...
Well-differentiated thyroid cancer most frequently occurs in premenopausal women. Greater exposure to estrogens may be a risk factor for cancer. To investigate the role of cancer, spot urine sample was obtained from 40 women with and age-matched controls. Thirty-eight estrogen metabolites, conjugates DNA adducts were analyzed by using ultraperformance liquid chromatography/tandem mass spectrometry ratio metabolites calculated each sample. The depurinating estrogen-DNA significantly differed...
The selectivity and beneficial effects of prebiotics are mainly dependent on composition glycosidic linkage among monosaccharide units. This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 β-1,3 linkages novel combination GOS inulin in cancer prevention. objective present explore role against various biomarkers colorectal (CRC) incidence aberrant crypt foci (ACF) a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model. Prebiotic treatments...
Abstract Nebraska's age‐adjusted incidence rates for childhood cancers are among the highest in US. Previous studies indicated associations between agrichemical exposures (atrazine and nitrates) pediatric cancer rate, assuming single pollutant exposure. We evaluated joint association agricultural mixture cancer. Agrichemical at a county scale were quantified using USGS Pesticide National Synthesis Project frequently applied pesticides from 1992 to 2014 93 Nebraska counties. Outcomes...
Despite ongoing efforts to employ structure-based methods discover targeted protein degraders (TPD), the prevailing strategy remains synthesizing a focused set of heterobifunctional compounds and screening them for target degradation.
A new guaianolide, taraxacin (1), and a known sesquiterpene ketolactone (2) have been isolated from an ethyl acetate-soluble part of methanolic extract Taraxacum wallichii. The structure 1 was established using NMR, MS, X-ray crystallographic methods. (13)C NMR data 2 is also being reported for the first time.
Greater exposure to estrogens is a risk factor for ovarian cancer. To investigate the role of in cancer, spot urine sample and saliva were obtained from 33 women with cancer 34 age-matched controls. Thirty-eight estrogen metabolites, conjugates DNA adducts analyzed samples using ultraperformance liquid chromatography/tandem mass spectrometry, ratio metabolites was calculated each sample. The depurinating estrogen-DNA significantly higher cases compared controls (p < 0.0001), demonstrating...
The neurotransmitter dopamine is oxidized to its quinone (DA-Q), which at neutral pH undergoes intramolecular cyclization by 1,4-Michael addition, followed oxidation form leukochrome, then aminochrome, and finally neuromelanin. At lower pH, the amino group of DA partially protonated, allowing competitive intermolecular addition with nucleophiles in DNA depurinating adducts, DA-6-N3Ade DA-6-N7Gua. Catechol estrogen-3,4-quinones react 4-hydroxyestrone(estradiol)-1-N3Ade [4-OHE1(E2)-1-N3Ade]...