A5034693987- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Bioinformatics and Genomic Networks
- Analytical Chemistry and Chromatography
- Glycosylation and Glycoproteins Research
- Renal cell carcinoma treatment
- Lung Cancer Research Studies
- Metabolism, Diabetes, and Cancer
- Tryptophan and brain disorders
- Lymphoma Diagnosis and Treatment
- Insect and Pesticide Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Phylogenetic Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Diagnosis and Treatment
Susan Thompson Buffett Foundation
2020-2025
Fred and Pamela Buffett Cancer Center
2020-2025
Nebraska Medical Center
2021-2024
University of Nebraska Medical Center
2018-2023
Go2 for Lung Cancer
2023
Westmead Hospital
2021
University of Hong Kong
2011-2020
University of Macau
2018-2020
The Eppley Laboratory (United States)
2020
University of Florida
2018-2019
Spexin (SPX) is a neuropeptide identified recently by bioinformatic approach. At present not much known about its biological actions, and comparative studies of SPX in nonmammalian species are still lacking. To examine the structure function fish model, was cloned goldfish found to be highly comparable with mammalian counterparts. As revealed NMR spectroscopies, composed an α-helix from Gln(5) Gln(14) flexible NH2 terminus Asn(1) Pro(4), molecular surface largely hydrophobic except for...
Abstract Purpose: Pancreatic ductal adenocarcinoma (PDAC) is a highly metastatic disease that can be separated into distinct subtypes based on molecular signatures. Identifying PDAC subtype-specific therapeutic vulnerabilities necessary to develop precision medicine approaches treat PDAC. Experimental Design: A total of 56 liver metastases were obtained from the UNMC Rapid Autopsy Program and analyzed with quantitative proteomics. identified by principal component analysis protein expression...
A novel fully automatable two-dimensional liquid chromatography (2DLC) platform has been integrated into a modified commercial off-the-shelf LC instrument, incorporating porous graphitic carbon (PGC) separation and conventional low-pH reversed-phase (RP) for both proteomics N-glycomics analyses; the dual-trap column configuration of this offers desirable high-throughput analyses with almost no idle time, in addition to miniaturized setup simplified operation. The total run time per analysis...
Abstract Background Siamese fighting fish Betta splendens are notorious for their aggressiveness and accordingly have been widely used to study aggression. However, the lack of a reference genome has, date, limited understanding genetic basis aggression in this species. Here, we present first assembly fish. Findings Frist, sequenced de novo assembled 465.24-Mb B. variety Giant, with weighted average (N50) scaffold size 949.03 Kb an N50 contig 19.01 Kb, covering 99.93% estimated size. To...
Abstract Introduction. Metastasis is the major cause of mortality in patients with solid tumors. Our group has identified precision therapeutic inhibitors cell motility and shown their ability to prevent metastasis. In striving move novel first-in-class therapy into humans, it important identify biomarkers efficacy. As β1 β5 integrins mediate prostate cancer migration, they are strong biomarker candidates. While our anti-motility agent, KBU2046, inhibits motility, its effects on integrin...
Abstract TBN, a novel tetramethylpyrazine derivative armed with powerful free radical-scavenging nitrone moiety, has been reported to reduce cerebral infarction in rats through multi-functional mechanisms of action. Here we study the therapeutic effects TBN on non-human primate model stroke. Thirty male Cynomolgus macaques were subjected stroke 4 hours ischemia and then reperfusion. injected intravenously at 3 or 6 after onset ischemia. Cerebral was examined by magnetic resonance imaging 1...
// Pauline Zaenker 1 , Johnny Lo 2 Robert Pearce 3 Phillip Cantwell Lester Cowell 4 Mark Lee 5 Christopher Quirk 6 Henry Law 7 Elin Gray and Mel Ziman 1, 8 School of Medical Health Sciences, Edith Cowan University, Joondalup, Perth, WA, Australia Science, Hollywood Specialist Centre, Private Hospital, Nedlands, Level Melanoma, Fremantle, St John God Subiaco, Dermatology Group, Ardross, Skin Check Inglewood, Department Pathology Laboratory Medicine, The University Western Australia, Crawley,...
In this study, we observed unprecedented cleavages of the C β -C γ bonds tryptophan residue side chains in a series hydrogen-deficient tryptophan-containing peptide radical cations (M •+ ) during low-energy collision-induced dissociation (CID).We used CID experiments and theoretical density functional theory (DFT) calculations to study mechanism bond cleavage, which forms [M -116] + ions.The formation an α-carbon intermediate at for subsequent cleavage is analogous that occurring leucine...
In vitro studies of liver stage (LS) development the human malaria parasite Plasmodium falciparum are technically challenging; therefore, fundamental questions about hepatocyte receptors for invasion that can be targeted to prevent infection remain unanswered. To identify novel and further understand susceptibility P. sporozoite invasion, we created an optimized in system by mimicking vivo conditions using subcloned HC-04.J7 cell line supports mean rates 3-5% early exoerythrocytic forms—a 3-...
We developed a novel online MDLC platform that integrates dual-trap configuration and two separation technologies into single automated commercial platform.
Stroke is one of the main causes mortality and long-term disability worldwide. The pathophysiological mechanisms underlying this disease are not well understood, particularly in chronic phase after initial ischemic episode. In study, a Macaca fascicularis stroke model consisting two sample groups, as determined by MRI-quantified infarct volumes measure severity 28 days episode, was evaluated using qualitative quantitative proteomics analyses. By multiple online multidimensional liquid...
(R)-Oxyphylla A, a natural product isolated from Alpinia oxyphylla Miquel as food and medicinal plant, has been reported previously novel chiral compound that possesses potential therapeutic value for Parkinson's disease (PD). A high-performance liquid chromatography–multiple reaction monitoring–mass spectrometry method was developed to separate enantiomers identify the presence of (S)-oxyphylla first time. Twelve samples dried A. fruits were analyzed in which large variation abundance...
Protein tyrosine nitration (PTN) is a signature hallmark of radical-induced nitrative stress in wide range pathophysiological conditions, with naturally occurring abundances at substoichiometric levels. In this present study, fully automated four-dimensional platform, consisting high-/low-pH reversed-phase dimensions two additional complementary, strong anion (SAX) and cation exchange (SCX), chromatographic separation stages inserted tandem, was implemented for the simultaneous mapping...
Pancreatic Ductal adenocarcinoma (PDAC) is an aggressive cancer commonly exhibiting KRAS-activating mutations. Alcohol contributes to the risk of developing PDAC in humans, and murine models have shown alcohol consumption context KRAS mutation pancreas promotes development PDAC. The molecular signatures cells altered by exposure mutant could identify pathways related etiology In this study, we evaluated combined effects status on transcriptome proteome pancreatic HPNE cell models. These...
Hyperinsulinemia affects 72% of Fanconi anemia (FA) patients and an additional 25% experience lowered glucose tolerance or frank diabetes. The underlying molecular mechanisms contributing to the dysfunction FA pancreas β cells is unknown. Therefore, we sought evaluate functional role FANCA, most commonly mutated gene in FA, glucose-stimulated insulin secretion (GSIS). This study reveals that FANCA FANCB knockdown impairs GSIS human cell line EndoC-βH3. To identify potential pathways by which...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Determination of Oxyphylla A Enantiomers in the Fruits Alpinia oxyphylla by Chiral High-Performance Liquid Chromatography–Multiple Reaction Monitoring–Mass Spectrometry Method and Comparison Their In Vivo Biological ActivitiesYan ChenYan ChenMore Yan Chen, Guohui LiGuohui LiMore Li, Henry Chun Hin LawHenry LawMore Law, Huanxian ChenHuanxian Simon Ming-Yuen Lee*Simon LeeMore...
ABSTRACT RNA polymerase II subunit A Carboxy-Terminal Domain Phosphatase 1 (CTDP1), a member of the haloacid dehalogenase superfamily phosphatases, has defined role in transcriptional regulation, but emerging evidence suggests an expanded functional repertoire cell cycle and DNA damage response. In humans, splice site mutation CTDP1 gives rise to rare Congenital Cataracts Facial Dysmorphism Neuropathy syndrome, recent from our lab indicates is required for breast cancer growth proliferation....
Paraquat is a potent superoxide (O₂
<h3>Introduction</h3> Surgical resection is a treatment of choice for patients with early stage lung cancer and physiological measurements are routinely used to help predict post-operative risk, particularly in the ‘high-risk’ patient group. At present, there lack concordance between current available guidelines incorporating use such parameters aimed guide decisions on surgery high-risk cancer. As result, decision operate will differ particular depending which consulted. We aim identify...
Abstract Introduction: Metastasis is a leading cause of death in patients with solid tumors. Our group has discovered KBU2046, selective inhibitor cell motility (Nature Communications 2018). We conducted time-course immunofluorescent staining to investigate the impact KBU2046 on adhesion and components human prostate cancer cells. objective was compare our findings proteomic analysis results order gain insights into novel molecular mechanisms modulating motility. Experimental Procedures:...