A5055813373- Muscle Physiology and Disorders
- Hedgehog Signaling Pathway Studies
- Adipose Tissue and Metabolism
- Mesenchymal stem cell research
- Exercise and Physiological Responses
- Electrochemical Analysis and Applications
- Neuroscience and Neuropharmacology Research
- RNA Interference and Gene Delivery
- Neurotransmitter Receptor Influence on Behavior
- Cardiovascular Effects of Exercise
- Hydrogen's biological and therapeutic effects
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Congenital limb and hand anomalies
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Cancer Diagnosis and Treatment
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Genetic and Kidney Cyst Diseases
- Fatty Acid Research and Health
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Drug Transport and Resistance Mechanisms
- Lipid metabolism and biosynthesis
University of Florida
2019-2024
Instituto de Neurología Cognitiva
2021-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2021-2023
Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia
2021-2023
Florida College
2021
Abstract Skeletal muscle has the remarkable ability to regenerate. However, with age and disease strength function decline. Myofiber size, which is affected by injury disease, a critical measurement assess health. Here, we test apply Cellpose, recently developed deep learning algorithm, automatically segment myofibers within murine skeletal muscle. We first show that tissue fixation necessary preserve cellular structures such as primary cilia, small antennae, adipocyte lipid droplets....
Successful muscle regeneration relies on the interplay of multiple cell populations. However, signals required for this coordinated intercellular crosstalk remain largely unknown. Here, we describe how Hedgehog (Hh) signaling pathway controls fate fibro/adipogenic progenitors (FAPs), cellular origin intramuscular fat (IMAT) and fibrotic scar tissue. Using conditional mutagenesis pharmacological Hh modulators in vivo vitro, identify DHH as key ligand that acts a potent adipogenic brake by...
Abstract Intramuscular fat (IMAT) infiltration, pathological adipose tissue that accumulates between muscle fibers, is a shared hallmark in diverse set of diseases including muscular dystrophies and diabetes, spinal cord rotator cuff injuries, as well sarcopenia. While the mouse has been an invaluable preclinical model to study skeletal diseases, they are also resistant IMAT formation. To better understand this feature, adequate pre-clinical recapitulates human disease necessary. address...
In vitro studies of liver stage (LS) development the human malaria parasite Plasmodium falciparum are technically challenging; therefore, fundamental questions about hepatocyte receptors for invasion that can be targeted to prevent infection remain unanswered. To identify novel and further understand susceptibility P. sporozoite invasion, we created an optimized in system by mimicking vivo conditions using subcloned HC-04.J7 cell line supports mean rates 3-5% early exoerythrocytic forms—a 3-...
SUMMARY With age and disease, skeletal muscle is progressively lost replaced by fibrotic scar intramuscular adipose tissue (IMAT). While strongly correlated, it remains unclear whether IMAT has a functional impact on muscle. In the present study, we evaluated effects of during injury creating mouse model where cellular origin IMAT, fibro/adipogenic progenitors (FAPs), are prevented from differentiating into adipocytes (FATBLOCK model). We found that blocking after an adipogenic allowed to...
Monoamine neurotransmitters are associated with numerous neurologic and psychiatric ailments. Animal models of such conditions have shown alterations in monoamine neurotransmitter release uptake dynamics. Technically complex methods as electrophysiology, Fast Scan Cyclic Voltammetry (FSCV), imaging, vivo microdialysis, optogenetics, or use radioactivity required to study function. The method presented here is an optimized two-step approach for detecting acute brain slices using a 48-well...
Abstract Successful muscle regeneration relies on the interplay of multiple cell populations. However, signals required for this coordinated intercellular crosstalk remain largely unknown. Here, we describe how Hedgehog (Hh) signaling pathway controls fate fibro/adipogenic progenitors (FAPs), cellular origin intramuscular fat (IMAT) and fibrotic scar tissue. Using conditional mutagenesis pharmacological Hh modulators in vivo vitro , identify DHH as key ligand that acts a potent adipogenic...
ABSTRACT Skeletal muscle has the remarkable ability to regenerate. However, with age and disease strength function decline. Myofiber size, which is affected by injury disease, a critical measurement assess health. Here, we test apply Cellpose, recently developed deep learning algorithm, automatically segment myofibers within murine skeletal muscle. We first show that tissue fixation necessary preserve cellular structures such as primary cilia, small antennae, adipocyte lipid droplets....
Background : Mesenchymal stem cells (MSCs) can be activated by different bacterial toxins. Lipopolysaccharides and Shiga Toxin (Stx) are the main toxins necessary for hemolytic uremic syndrome development. The etiological event in this disease is endothelial damage that causes glomerular destruction. Considering repairing properties of MSC, we aimed to study response MSC derived from induced pluripotent (iPSC-MSC) LPS and/or Stx its effect on restoration injured cells. Methods iPSC-MSC were...
Skeletal muscle has a remarkable ability to regenerate upon injury. However, during aging and in diseases such as muscular dystrophies diabetes, tissue is replaced by fibrotic scar fat process called fatty fibrosis. Currently, there are no known treatments prevent fibrosis or the decline mass function. Our lab others have shown that ectopic activation of Hedgehog (Hh) pathway accelerated regeneration after an acute injury preserved function with age disease. In addition, our demonstrated Hh...
Background: Mesenchymal Stem Cells can be activated and respond to different bacterial toxins. Lipopolysaccharides (LPS) Shiga Toxin (Stx) are the two main toxins present in Hemolytic Uremic Syndrome (HUS) that cause endothelial damage. In this work we aimed study response of iPSC-MSC LPS and/or Stx its effect on restoration injured cells. Methods: were used as a source mesenchymal stem cells (MSC) Human Microvascular Endothelial Cells-1 (HMEC-1) treated or not with or/Stx. For some...
Mesenchymal Stem Cells derived from induced Pluripotent cells (iPSC-MSC) have become a promising alternative to classical in regenerative medicine. Their properties -as immunomodulatory and capacities-are part due the secretion of Extracellular Vesicles (EVs). Small EVs (sEVs) with sizes that range 50 120 nm contain proteins, lipids, nucleic acids exert role cellular communication. content will depend on cell origin its physiological state, thus message they convey might change response...
Monoamine neurotransmitters are associated with numerous neurologic and psychiatric ailments. Animal models of such conditions have shown alterations in monoamine neurotransmitter release uptake dynamics. Technically complex methods as electrophysiology, Fast Scan Cyclic Voltammetry (FSCV), imaging, vivo microdialysis, optogenetics, or use radioactivity required to study function. The method presented here is an optimized two-step approach for detecting acute brain slices using a 48-well...