A5043269288- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Cardiac electrophysiology and arrhythmias
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Electrostatics and Colloid Interactions
- Nanotechnology research and applications
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Hearing, Cochlea, Tinnitus, Genetics
- Characterization and Applications of Magnetic Nanoparticles
- Cell Image Analysis Techniques
- Anodic Oxide Films and Nanostructures
Google (United States)
2021-2022
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, large variability in success rate signal strength hindered from being broadly adopted for proarrhythmia drug assessment....
AI models have been proposed for hypothesis generation, but testing their ability to drive high-impact research is challenging, since an AI-generated can take decades validate. Here, we challenge the of a recently developed LLM-based platform, co-scientist, generate high-level hypotheses by posing question that took years resolve experimentally remained unpublished: How could capsid-forming phage-inducible chromosomal islands (cf-PICIs) spread across bacterial species? Remarkably,...
We demonstrate early progress toward constructing a high-throughput, single-molecule protein sequencing technology utilizing barcoded DNA aptamers (binders) to recognize terminal amino acids of peptides (targets) tethered on next-generation chip. binders deposit unique, acid-identifying barcodes the The end goal is that, over multiple binding cycles, sequential chain will identify acid sequence peptide. Toward this, we successful target identification with two sets target-binder pairs:...
Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps discovery DNA aptamers with specificity two consecutive N-terminal amino acids (AAs) small peptide (8–10 acids). This bead-based method may be adapted applications requiring which recognize specific portion desired target. For...
Abstract Aptamers are single-stranded nucleic acid ligands that bind to target molecules with high affinity and specificity. They typically discovered by searching large libraries for sequences desirable binding properties. These libraries, however, practically constrained a fraction of the theoretical sequence space. Machine learning provides an opportunity intelligently navigate this space identify high-performing aptamers. Here, we present step-by-step protocol utilizing particle display...
Despite a need for high-throughput protein sequencing, existing methods are not capable of sequencing the entire human proteome. We demonstrate early progress toward constructing components technology that requires sequential capture and recording binding over many cycles. The Barcode Cycle Sequencing (BCS) assay uses DNA-barcoded binders to construct chain barcodes directly onto DNA chip used identify target. demonstrated successful barcode DNA-barcode DNA-DNA nanobody-protein pairs. For...
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. However, conventional patch clamp techniques for measuring iAPs either laborious and low throughput or not suitable electrically connected In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel minimally-invasive iAP...