A5024879980- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Photoreceptor and optogenetics research
- Pluripotent Stem Cells Research
- Enzyme Structure and Function
- Force Microscopy Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Cellular Mechanics and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Electrochemical Analysis and Applications
- Neuropeptides and Animal Physiology
- Magnetic and Electromagnetic Effects
- Bacteriophages and microbial interactions
- Advanced Sensor and Energy Harvesting Materials
- Cancer Cells and Metastasis
- Biochemical and Molecular Research
- Semiconductor materials and devices
- Molecular Junctions and Nanostructures
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Tuberous Sclerosis Complex Research
- Neuroscience and Neuropharmacology Research
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Hedgehog Signaling Pathway Studies
University of California, Berkeley
2024-2025
Stanford University
2018-2024
Organogenesis (United States)
2023
Neurosciences Institute
2022-2023
Lawrence Berkeley National Laboratory
2018
Cornell University
2016
Significance The large mechanical discrepancy at the cell–electrode interface (∼6 orders of magnitude difference in Young’s moduli) is known to significantly affect cell’s behavior and its mechanotransduction pathways. To date, moduli electrode materials do not match those cells, due limited availability required electronic for microfabrication. Here, we present a soft micropillar array made electronically conductive hydrogel as general tool electrophysiological recording. three-dimensional...
Both substrate stiffness and surface topography regulate cell behavior through mechanotransduction signaling pathways. Such intertwined effects suggest that engineered topographies might substitute or cancel the of in biomedical applications. However, mechanisms by which cells recognize topographical features are not fully understood. Here we demonstrate presence nanotopography drastically alters such neurons stem cultured on rigid glass substrates behave as if they were soft hydrogels. With...
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, large variability in success rate signal strength hindered from being broadly adopted for proarrhythmia drug assessment....
Abstract Tuberous Sclerosis Complex (TSC) is a genetic neurodevelopmental disorder associated with early onset epilepsy, intellectual disability and neuropsychiatric disorders. A hallmark of the cortical tubers, which are focal malformations brain development that contain dysplastic cells hyperactive mTORC1 signaling. One barrier to developing therapeutic approaches understanding origins tuber lack model system recapitulates this pathology. To address this, we established genetically mosaic...
Organoids and assembloids have emerged as a promising platform to model aspects of nervous system development. Longterm, minimally-invasive recordings in these multi-cellular systems are essential for developing disease models. Current technologies, such patch-clamp, penetrating microelectrodes, planar electrode arrays substrate-attached flexible electrodes, do not, however, allow chronic recording organoids suspension, which is necessary preserve their architecture. Inspired by the art...
Magnetogenetics is a new field that leverages genetically encoded proteins and protein assemblies are sensitive to magnetic fields study manipulate cell behavior. Theoretical studies show many proposed magnetogenetic do not contain enough iron generate substantial forces. Here, we have engineered ferritin-containing crystal grows inside mammalian cells. Each of these crystals contains more than 10 million ferritin subunits capable mineralizing amounts iron. When isolated from cells loaded...
Summary Neuropeptides are the largest class of neuromodulators. It has been shown that subpopulations dopamine neurons express mRNA for neuropeptide Gastrin-releasing peptide (GRP); however, its functional relevance in dopaminergic circuits is unknown. Here, we find GRP receptor (GRPR) present nucleus accumbens medial shell (NAc MSh), which targeted by GRP-expressing midbrain as well glutamatergic inputs from hippocampus and amygdala. We show NAc MSh GRPR-positive cells a subpopulation D2...
Chemists have long preferred the Si(111) surface for chemical functionalization, as a simple aqueous etch can be used to produce ideal, atomically flat H/Si(111) surfaces subsequent reactions. In contrast, industry-standard etches rough H/Si(100) terminated by nanohillocks. The recent discovery of an that produces morphologically controlled with near or "half-flat" morphology challenges assumption is inherently preferable starting point functionalization. This study shows alkyl...
Advances in protein engineering have enabled the production of self-assembled crystals within living cells. Our recent publication demonstrates ftn-PAK4, which is a ferritin-containing crystal that can mineralize iron and become magnetic when isolated. We developed an optimized protocol for isolation PAK4-based crystals. The are first grown low-passage HEK293T cells, released using lysis buffer containing NP-40 DNase, collected under careful centrifugation conditions. maximizes purity yield...
Abstract During brain development, neural progenitor cells first produce neurons, then astrocytes and other glial cell types, which provide important trophic support shape neuronal development function. Intrinsic genetic programs interact with extracellular signals to control fate, resulting in temporally segregated periods of neurogenesis gliogenesis. Animal models have implicated STAT3 as an driver astrogenesis; however, the signaling pathways that differentiation during human are less...
Advances in tridimensional (3D) culture approaches have led to the generation of organoids that recapitulate cellular and physiological features domains human nervous system. Although microelectrodes been developed for long-term electrophysiological interfaces with neural tissue, studies between free-floating remain limited. In this study, we report a stretchable, soft mesh electrode system establishes an intimate vitro electrical interface neurons 3D organoids. Our is constructed...
Abstract Both substrate stiffness and surface topography regulate cell behavior through mechanotransduction signaling pathways. Such intertwined effects suggest that engineered topographies might substitute or cancel the of in biomedical applications. However, mechanisms by which cells recognize topographical features are not fully understood. Here we demonstrate presence nanotopography drastically alters such neurons stem cultured on rigid glass substrates behave as if they were soft...
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. However, conventional patch clamp techniques for measuring iAPs either laborious and low throughput or not suitable electrically connected In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel minimally-invasive iAP...