A5047830370- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Parkinson's Disease Mechanisms and Treatments
- Chromatin Remodeling and Cancer
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- Drug Transport and Resistance Mechanisms
- Acupuncture Treatment Research Studies
- Malaria Research and Control
- HIV/AIDS drug development and treatment
- Pharmacological Effects of Natural Compounds
- Ion channel regulation and function
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Histone Deacetylase Inhibitors Research
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Cancer Genomics and Diagnostics
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2024-2025
University of Electronic Science and Technology of China
2024-2025
Heilongjiang University of Chinese Medicine
2024-2025
Anhui University
2025
Liaoning University of Traditional Chinese Medicine
2025
Yumanity Therapeutics (United States)
2021-2024
Sixth Affiliated Hospital of Sun Yat-sen University
2023-2024
Sun Yat-sen University
2023-2024
Alpha-synuclein (αS) is a conformationally plastic protein that reversibly binds to cellular membranes. It aggregates and genetically linked Parkinson's disease (PD). Here, we show αS directly modulates processing bodies (P-bodies), membraneless organelles function in mRNA turnover storage. The N terminus of αS, but not other synucleins, dictates mutually exclusive binding either membranes or P-bodies the cytosol. associates with multiple decapping proteins close proximity on Edc4 scaffold....
We characterized metabolic and mitogenic signaling pathways in isolated skeletal muscle from well-matched type 2 diabetic control subjects. Time course studies of the insulin receptor, receptor substrate (IRS)-1/2, phosphatidylinositol (PI) 3-kinase revealed that signal transduction through this pathway was engaged between 4 40 min. Insulin-stimulated (0.6-60 nmol/l) tyrosine phosphorylation beta-subunit, mitogen-activated protein (MAP) kinase phosphorylation, glycogen synthase activity were...
The serine/threonine kinase Akt (PKB/Rac) has been implicated as playing a role in the insulin-signaling pathway to glucose transport. Little is known regarding regulation of activity insulin-sensitive tissues, such skeletal muscle, or whether this altered insulin-resistant states NIDDM. We examined effect insulin on muscle from six NIDDM patients and healthy subjects. Whole-body sensitivity, assessed by euglycemic-hyperinsulinemic clamp, was significantly lower subjects (P < 0.001),...
The molecular signaling mechanisms by which muscle contractions lead to changes in glucose metabolism and gene expression remain largely undefined. We assessed whether exercise activates MAP kinase proteins (ERK1/2, SEK1, p38 kinase) as well Akt PYK2 skeletal from healthy volunteers obtained during after one-leg cycle ergometry at ∼70% VO2max. Exercise led a marked increase ERK1/2 phosphorylation, rapidly decreased resting levels upon recovery. increased phosphorylation of SEK1 lesser extent...
Wumei Pill (WMP), a famous herbal formula, has been widely used to treat digestive system diseases in clinical practice China for centuries. We have found correlation between the indications of WMP and typical symptoms pancreatic neoplasms. However, pharmacological mechanisms still remain unknown. In present work, we network method predict its underlying complex mechanism treating Firstly, obtained relative compounds based on TCMSP database, TCM database@Taiwan TCMID database collected...
Objectives: Pathological biomarkers and mechanisms of dengue infection are poorly understood.We investigated a new serum biomarker using miRNAs performed further correlation analysis in dengue-infected patients.Methods: Expression levels broad-spectrum samples from three patients with virus type 1 (DENV-1) healthy volunteers were separately analyzed miRNA PCR arrays.The expressions the five selected verified by qRT-PCR sera 40 DENV-1 compared those 32 controls.Receiver operating...
Abstract Drug-induced cardiotoxicity arises primarily when a compound alters the electrophysiological properties of cardiomyocytes. Features intracellular action potentials (iAPs) are powerful biomarkers that predict proarrhythmic risks. In last decade, number vertical nanoelectrodes have been demonstrated to achieve parallel and minimally-invasive iAP recordings. However, large variability in success rate signal strength hindered from being broadly adopted for proarrhythmia drug assessment....
Continuous de novo fatty acid synthesis is required for the biosynthetic demands of tumor. FBXW7 a highly mutated gene in CRC, but its biological functions cancer are not fully characterized. Here, we report that FBXW7β, isoform located cytoplasm and frequently an E3 ligase synthase (FASN). Cancer-specific FBXW7β mutations could degrade FASN can lead to sustained lipogenesis CRC. COP9 signalosome subunit 6 (CSN6), oncogenic marker increases via interacting with stabilizing FASN. Mechanistic...
Abstract Cholesterol metabolism has important roles in maintaining membrane integrity and countering the development of diseases such as obesity cancers. Cancer cells sustain cholesterol biogenesis for their proliferation microenvironment reprograming even when sterols are abundant. However, efficacy targeting cancer treatment is always compromised. Here it shown that CSN6 elevated HCC a positive regulator hydroxymethylglutaryl‐CoA synthase 1 (HMGCS1) mevalonate (MVA) pathway to promote...
The heterogeneity of protein-rich inclusions and its significance in neurodegeneration is poorly understood. Standard patient-derived iPSC models develop neither reproducibly nor a reasonable time frame. Here, we developed screenable "inclusionopathy" utilizing piggyBac or targeted transgenes to rapidly induce CNS cells that express aggregation-prone proteins at brain-like levels. Inclusions their effects on cell survival were trackable single-inclusion resolution. Exemplar cortical neuron...
Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) is a key regulator of synaptic responses in the postsynaptic density, but understanding its mechanisms action presynaptic neuron incomplete. Here we show that CaMKII constitutively associates with and modulates voltage-gated calcium (Ca V )2.1 channels conduct P/Q type currents initiate transmitter release. Both exogenous brain-specific inhibitors accelerate voltage-dependent inactivation, cause negative shift voltage dependence reduce...
Abstract Human glucose transporters (GLUTs) are responsible for cellular uptake of hexoses. Elevated expression GLUTs, particularly GLUT1 and GLUT3, is required to fuel the hyperproliferation cancer cells, making GLUT inhibitors potential anticancer therapeutics. Meanwhile, inhibitor-conjugated insulin being explored mitigate hypoglycemia side effect therapy in type 1 diabetes. Reasoning that exofacial GLUT1/3 may be favored therapeutic applications, we report here engineering a GLUT3...
To investigate the relations of Triglyceride glucose (TyG) index with cerebral small vessel disease (CSVD) burden and cognitive function in aged patients type 2 diabetes mellitus (T2DM).A total 308 elderly T2DM were included this retrospective study. The standardized Chinese version Mini-Mental State Examination was used to assess function. CSVD score assessed by combining four imaging markers CSVD, including presence white matter hyperintensity, microbleeds deep, lacunes enlarged...
Protein localization is crucial for cellular morphogenesis and intracellular signal transduction cascades. Here we describe an interaction between two membrane proteins expressed in different cells of the Bacillus subtilis sporangium, mother cell protein SpoIIIAH forespore SpoIIQ. We used affinity chromatography, coimmunoprecipitation, yeast two-hybrid system to demonstrate that extracellular domains these interact, tethering sporulation septum, directing its assembly with SpoIIQ into...
One of the largest families transcriptional regulators contains WRKY proteins. They play important roles in plant defense responses. In this study, a novel gene, CsWRKY2, was isolated from tea [Camellia sinensis (L.) O. Kuntze] plant. The full-length cDNA CsWRKY2 2 050 bp length and encoded 522-amino acid peptide chain containing two typical domains zinc finger motifs, suggesting that member group I family. A subcellular localization assay shows localized to nucleus. Real time qPCR analysis...
Objectives: Circulating microRNAs (miRNAs) play critical roles in pathogen-host interactions.Aberrant miRNA expression profiles might have specific characteristics for virus strains, and could serve as noninvasive biomarkers screening diagnosing infectious diseases.In this study, we aimed to find new potential of hepatitis C (HCV) infection.Methods: Expression levels broad-spectrum miRNAs serum samples from 10 patients with HCV viremia healthy volunteers were analyzed using PCR...
The present study investigated the synergic effects and potential mechanisms of action Astragalus polysaccharides (APS) combined with Crataegus flavonoids (CF) in treatment type 1 diabetes. Diabetes was induced by intraperitoneal injection 100 mg/kg streptozotocin mice. Normal untreated diabetic control mice were used, CF‑treated (200 mg/kg/day), APS‑treated APS + CF (AC)‑treated mg/kg/day each) metformin‑treated mg/kg/day) orally administrated appropriate therapeutic agent for 4 weeks....