A5008394989- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Glycosylation and Glycoproteins Research
- Lipid metabolism and biosynthesis
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Galectins and Cancer Biology
- Adipose Tissue and Metabolism
- Chemical Synthesis and Analysis
- Enzyme Production and Characterization
- Protein Tyrosine Phosphatases
- Pharmacology and Obesity Treatment
- Polysaccharides and Plant Cell Walls
- Hemoglobin structure and function
- Insurance and Financial Risk Management
- Biopolymer Synthesis and Applications
- Photochromic and Fluorescence Chemistry
- Glycogen Storage Diseases and Myoclonus
- Metabolism, Diabetes, and Cancer
- Ion Transport and Channel Regulation
- Calcium signaling and nucleotide metabolism
Sanofi (Germany)
2004-2023
University of Manchester
2010-2012
Manchester University
2010
ThyssenKrupp (Germany)
2008
Centre National de la Recherche Scientifique
2004-2006
Institute of Protein Biochemistry
2006
Laboratoire de Biochimie
2006
Sanofi (France)
2006
University of Freiburg
1990-1995
Abstract Human glucose transporters (GLUTs) are responsible for cellular uptake of hexoses. Elevated expression GLUTs, particularly GLUT1 and GLUT3, is required to fuel the hyperproliferation cancer cells, making GLUT inhibitors potential anticancer therapeutics. Meanwhile, inhibitor-conjugated insulin being explored mitigate hypoglycemia side effect therapy in type 1 diabetes. Reasoning that exofacial GLUT1/3 may be favored therapeutic applications, we report here engineering a GLUT3...
Abstract A single high‐affinity fatty acid binding site in the important human transport protein serum albumin (HSA) is identified and characterized using an NBD (7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)‐C 12 acid. This ligand exhibits a 1:1 stoichiometry its HSA complex with high site‐specificity. The dissociation constant determined by titration experiments as well radioactive equilibrium dialysis. Competition known HSA‐binding drugs warfarin ibuprofen confirm new to be different from...
Hormone-sensitive lipase (HSL) contributes importantly to the mobilization of fatty acids from triacylglycerols stored in adipocytes, which provide main source energy mammals. On basis amino acid sequence alignments and three-dimensional structures, this enzyme was previously found be a suitable template for defining family serine carboxylester hydrolases. In study, HSL members are characterized rather on their inhibition by 5-methoxy-3-(4-phenoxyphenyl)-3H-[1,3,4]oxadiazol-2-one (compound...
Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites mode of interaction with molecular entities, so that a resolution the individual binding in terms spatial localization as well association dissociation kinetics is required for meaningful description. Here we describe trichromatic fluorescent binding- displacement assay simultaneous monitoring three important transporter protein human...
Hormone-sensitive lipase (HSL) is thought to contribute importantly the mobilization of fatty acids from triacylglycerols (TAGs) stored in adipocytes, providing main source energy mammals. To investigate HSL substrate specificity more closely, we systematically assessed lipolytic activity recombinant human on solutions and emulsions various vinyl esters TAG substrates, using pH-stat assay technique. Recombinant partly soluble or was found range 35 90% maximum measured with same substrates...
Significance Lipid metabolism is a growing area of biochemical research because understanding these pathways could lead to treatments for metabolic disorders such as obesity and type 2 diabetes. To study lipid metabolism, researchers need tools identify quantitate glycerides, the main component animal fat. However, it can be difficult tell one glyceride apart from another subtly different using current analytical methods mass spectrometry. Thus, we developed method discriminating glycerides...
Saturation transfer difference (STD), inter-ligand NOEs (INPHARMA NMR), and docking calculations are reported for investigating specific binding sites of the high-affinity synthetic 7-nitrobenz-2-oxa-1,3-diazoyl-4-C12 fatty acid (NBD-C12 FA) with non-labeled human serum albumin (HSA) in competition drugs warfarin ibuprofen. A limited number negative interligand between NBD-C12 FA were interpreted terms a short-range allosteric competitive wide Sudlow's site II (FA7) Ser-202, Lys-199, Trp-214...
An approach for tracing the origin of submerged entry nozzle (SEN) clogging that occurs during continuous casting Al-killed steel is presented. This consists using stable oxygen isotope ratios. IR laser fluorination in combination with gas mass spectroscopy performed to determine composition alumina-rich precipitates (clogging) and possible sources like refractory materials, slags, process atmospheric oxygen. Three are identified. A quantitative model