A5034766855- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Influenza Virus Research Studies
- Drug-Induced Hepatotoxicity and Protection
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Liver physiology and pathology
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Child and Adolescent Health
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- Endoplasmic Reticulum Stress and Disease
- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Bioinformatics and Genomic Networks
- interferon and immune responses
- Cancer-related gene regulation
- Nanoparticle-Based Drug Delivery
National Center for Infectious Diseases
2025
National Clinical Research
2020-2025
First Affiliated Hospital Zhejiang University
2017-2024
Zhejiang University
2017-2024
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2018-2024
National Clinical Research Center for Digestive Diseases
2022-2024
MedStar Health
2022
Center for Innovation
2020
Indiana University
2018
Tsinghua University
2014-2016
Objectives: Pathological biomarkers and mechanisms of dengue infection are poorly understood.We investigated a new serum biomarker using miRNAs performed further correlation analysis in dengue-infected patients.Methods: Expression levels broad-spectrum samples from three patients with virus type 1 (DENV-1) healthy volunteers were separately analyzed miRNA PCR arrays.The expressions the five selected verified by qRT-PCR sera 40 DENV-1 compared those 32 controls.Receiver operating...
The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts understand the biological traits of newly identified human (HCoV-19) virus. In this mass spectrometry (MS)-based study, we reveal that out 21 possible glycosites in HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly oligomannose type. All seven glycosylation sites angiotensin I converting enzyme 2 (hACE2) were found be occupied, mainly complex N-glycans. However, did not...
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with high morbidity and mortality. Therefore, it very important to find potential biomarkers that can effectively predict prognosis progression HCC. Recent studies have shown anti-silencing function 1B (ASF1B) may be a new proliferative marker for tumor diagnosis prognosis. However, expression ASF1B in hepatocellular remain determined. In this study, integrated analysis Cancer Genome Atlas (TCGA), genotypic tissue...
Objectives: Circulating microRNAs (miRNAs) play critical roles in pathogen-host interactions.Aberrant miRNA expression profiles might have specific characteristics for virus strains, and could serve as noninvasive biomarkers screening diagnosing infectious diseases.In this study, we aimed to find new potential of hepatitis C (HCV) infection.Methods: Expression levels broad-spectrum miRNAs serum samples from 10 patients with HCV viremia healthy volunteers were analyzed using PCR...
BACKGROUND. HBV-related acute-on-chronic liver failure (HBV-ACLF) is hallmarked by high short-term mortality rates, calling for accurate prognostic biomarkers initial risk stratification.
Abstract Objectives In this study, we comprehensively analysed the role of ubiquitin‐specific protease 1(USP1) in hepatocellular carcinoma (HCC) using data from a set public databases. Materials and Methods We mRNA expression USP1 HCC subgroups Oncomine UALCAN. Survival analysis was conducted with Kaplan‐Meier Plotter database. The mutations were cBioPortal Catalogue Somatic Mutations Cancer Differential genes correlated WD repeat domain 48 ( WDR48 ) obtained LinkedOmics. knocked down small...
Partial liver resection is an established treatment for hepatic disorders. However, surgical bleeding, intra-abdominal adhesion and rapid regeneration are still major challenges after partial resection, associated with morbidity mortality. Herein, a biomimetic hybrid hydrogel, composed of oxidized hyaluronic acid, glycol chitosan MenSCs-derived conditioned medium (CM), presented to address these issues. The hydrogel formed through reversible Schiff base, possesses injectability self-healing...
Tumor metastasis is a major factor for poor prognosis of hepatocellular carcinoma (HCC), but the relationship between ubiquitination and need to be studied more systematically. We analyzed ubiquitinome HCC in this study have comprehensive insight into human metastasis.The protein levels 15 specimens with vascular invasion without were detected by ubiquitinome. Proteins significantly different HCCs used predict E3 ubiquitin ligases associated tumor metastasis. The topological network...
Rheumatoid arthritis (RA) is a debilitating systemic autoimmune disorder that significantly impairs quality of life. To elucidate the molecular alterations in RA progression, we performed comprehensive super-omics analyses on synovial tissues from patients with joint trauma, arthritis, and RA. These included transcriptomics, proteomics, metabolomics, microbiomics, fourteen PTMs (phosphorylation, acetylation, lactylation, O-GlcNAc glycosylation, arginine monomethylation, lysine dimethylation,...
Aim: To evaluate the levels of metabolites and cytokines in serum patients with severe non-severe idiosyncratic drug-induced liver injury (DILI) to identify biomarkers DILI severity. Methods: Gas chromatography–mass spectrometry (GC–MS) ultraperformance liquid (UPLC–MS) based metabolomic approaches were used metabolome samples from 29 severity grade 3 (non-severe), 27 4 (severe), 36 healthy control (HC). The total keratin-18 (K18), fragment K18, determined by enzyme-linked immunosorbent...
Abstract Artificial liver support systems (ALSS) are widely used to treat patients with hepatitis B virus-related acute-on-chronic failure (HBV-ACLF). The aims of the present study were investigate subgroups HBV-ACLF who may benefit from ALSS therapy, and relevant patient-specific factors. 489 ALSS-treated enrolled, served as derivation validation cohorts for classification regression tree (CART) analysis. CART analysis identified three factors prognostic survival: hepatic encephalopathy...
Increased oxidative stress levels play a key role in idiosyncratic drug-induced liver injury (DILI) pathogenesis. To investigated whether advanced oxidation protein products (AOPPs) and ischaemia-modified albumin (IMA) can be used to monitor DILI patients assess disease severity. We performed spectrophotometric assays AOPPs IMA 68 with severity grade 0-2 (non-severe group), 60 3-5 (severe 38 healthy controls. The results showed that baseline serum AOPPs/albumin IMA/albumin ratios were...
In this study, we aimed to investigate the effect and action mechanisms of pineapple leaf phenols (PLPs) on liver fat metabolism in high-fat diet-fed mice. Results show that PLP significantly reduced abdominal lipid accumulation The effects were comparable with those FB. Furthermore, at protein level, upregulated expression carnitine palmitoyltransferase 1 (CPT-1), whereas FB had no CPT-1 compared HFD controls. Regarding mRNA expression, mainly promoted CPT-1, PGC1a, UCP-1, AMPK...
Tectorigenin has received attention due to its antiproliferation, anti‐inflammatory, and antioxidant activities. In this study, we investigated the effects of tectorigenin on lipopolysaccharide (LPS)/D‐galactosamine(D‐GalN)‐induced fulminant hepatic failure (FHF) in mice LPS‐stimulated macrophages (RAW 264.7 cells). Pretreatment with significantly reduced serum levels alanine aminotransferase (ALT) aspartate (AST), histological injury, apoptosis, mortality FHF mice, by suppressing production...
Network cross-talks between microRNAs (miRNAs) and mRNAs may be useful to elucidate the pathological mechanisms of pancreatic islet cells in diabetic individuals. The aim present study was investigate miRNAs tissues streptozotocin-induced mice through microarray bioinformatic methods. Based on miRNA microarray, 64 upregulated 72 downregulated were observed compared normal controls. mRNA microarrray, 507 570 identified Notably, there 246 binding points mRNAs; simultaneously, 583 mRNAs. These...
Abstract Posttranslational modifications (PTMs) of proteins, particularly acetylation, phosphorylation, and ubiquitination, play critical roles in the host innate immune response. PTMs’ dynamic changes crosstalk among them are complicated. To build a comprehensive network inflammation-related we integrated data from whole-cell proteome (WCP), acetylome, phosphoproteome, ubiquitinome human mouse macrophages. Our datasets ubiquitination sites helped identify PTM within across proteins involved...
Abstract Background & Aims Non‐invasive assessment methods for liver fibrosis are urgently needed. The present study aimed to develop a novel diagnostic model staging in patients with chronic hepatitis B. Methods A cross‐sectional set of 417 B who underwent biopsy was enrolled and the METAVIR score adopted as reference staging. Results Among thyroid hormones, only level free tetraiodothyronine ( FT 4) decreased gradually P < .001). FibroStage, diagnosis that incorporates data on 4,...
The pathology and immunological responses to hemagglutinin (HA) from H7N9 avian influenza viruses in humans remain unclear. present study aimed investigate the proinflammatory activity of HA protein obtained mechanisms underlying homeostasis microRNAs (miRNAs) response inflammatory stimuli. expression factors miRNAs was assayed THP‑1 cells using reverse transcription‑quantitative polymerase chain reaction. Results showed that significantly increased interleukin (IL)‑1α, IL‑1β IL‑6 cells....
Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a life-threatening disease with high mortality rate; the systemic inflammatory response plays vital role in progression. We aimed to determine if miRNA-mRNA co-regulatory network exists peripheral blood mononuclear cells (PBMCs) of HBV-ACLF patients, which might be important for prognosis.In patients meeting COSSH-ACLF criteria, age, cirrhosis and INR were independent risk factors 28-day 90-day poor prognosis. COSSH-ACLFs...