A5087935264- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Hepatitis C virus research
- Drug-Induced Hepatotoxicity and Protection
- Inflammatory Biomarkers in Disease Prognosis
- Organ Transplantation Techniques and Outcomes
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Disaster Response and Management
- Genomics, phytochemicals, and oxidative stress
- Mesenchymal stem cell research
- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- Pleural and Pulmonary Diseases
- Mycobacterium research and diagnosis
- Pharmacogenetics and Drug Metabolism
- Liver physiology and pathology
- Pneumothorax, Barotrauma, Emphysema
- Streptococcal Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
Nanchang University
2020-2025
First Affiliated Hospital of Jiangxi Medical College
2025
First Affiliated Hospital of Nanchang University
2020-2024
First Affiliated Hospital Zhejiang University
2019-2023
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2016-2023
Nanfang Hospital
2020-2021
Southern Medical University
2020-2021
Zhejiang University
2016-2020
Center for Innovation
2017-2020
National Clinical Research
2020
BACKGROUND. HBV-related acute-on-chronic liver failure (HBV-ACLF) is hallmarked by high short-term mortality rates, calling for accurate prognostic biomarkers initial risk stratification.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by Dabie bandavirus (DBV). We report a case of DBV and Mycoplasma pneumoniae (MP) co-infection. Here we reported 57-year-old healthy male who was admitted the presentations fever, cough, hemoptysis, hypotension. Laboratory investigations revealed thrombocytopenia, leukopenia, organ dysfunction liver kidney. Seroconversion from IgM antibody to IgG recorded, SFTS confirmed through metagenomic...
Chronic HBV infection (CHB) can lead to acute-on-chronic liver failure (HBV-ACLF) characterized by high mortality.This study aimed reveal ACLF-related proteomic alterations, from which protein based diagnostic and prognostic scores for HBV-ACLF were developed.Methods: Ten healthy controls, 16 CHB, 19 according COSSH (Chinese group on the of severe hepatitis B) criteria enrolled obtain comprehensive portrait related initiation progression.Potential markers further selected organ specificity...
Aim: To evaluate the levels of metabolites and cytokines in serum patients with severe non-severe idiosyncratic drug-induced liver injury (DILI) to identify biomarkers DILI severity. Methods: Gas chromatography–mass spectrometry (GC–MS) ultraperformance liquid (UPLC–MS) based metabolomic approaches were used metabolome samples from 29 severity grade 3 (non-severe), 27 4 (severe), 36 healthy control (HC). The total keratin-18 (K18), fragment K18, determined by enzyme-linked immunosorbent...
Summary Background HBV‐related acute‐on‐chronic liver failure (HBV‐ACLF) deteriorates rapidly in the short term, which necessitates accurate initial clinical decision making. Aims To develop a novel prognostic score for patients with HBV‐ACLF and clarify role of thyroid hormones HBV‐ACLF. Methods A retrospective cohort 635 was enrolled to validate Additionally, cross‐sectional (n = 199) prospective longitudinal 56) were recruited association between hormone status 30‐day mortality Results...
Abstract Artificial liver support systems (ALSS) are widely used to treat patients with hepatitis B virus-related acute-on-chronic failure (HBV-ACLF). The aims of the present study were investigate subgroups HBV-ACLF who may benefit from ALSS therapy, and relevant patient-specific factors. 489 ALSS-treated enrolled, served as derivation validation cohorts for classification regression tree (CART) analysis. CART analysis identified three factors prognostic survival: hepatic encephalopathy...
Tectorigenin has received attention due to its antiproliferation, anti‐inflammatory, and antioxidant activities. In this study, we investigated the effects of tectorigenin on lipopolysaccharide (LPS)/D‐galactosamine(D‐GalN)‐induced fulminant hepatic failure (FHF) in mice LPS‐stimulated macrophages (RAW 264.7 cells). Pretreatment with significantly reduced serum levels alanine aminotransferase (ALT) aspartate (AST), histological injury, apoptosis, mortality FHF mice, by suppressing production...
Acute liver failure (ALF) is a fatal condition hallmarked by rapid development. The present study aimed to describe the dynamic alterations of serum proteins associated with ALF development, and seek for novel biomarkers ALF. Miniature pigs (n = 38) were employed establish models infusing d-galactosamine (GALN, 1.3 g/kg). A total 1310 compared in pooled samples 10) before 36 h after GALN administration through label-free quantitation (LFQ) based shotgun proteomics. Functional analysis...
Abstract Background & Aims Non‐invasive assessment methods for liver fibrosis are urgently needed. The present study aimed to develop a novel diagnostic model staging in patients with chronic hepatitis B. Methods A cross‐sectional set of 417 B who underwent biopsy was enrolled and the METAVIR score adopted as reference staging. Results Among thyroid hormones, only level free tetraiodothyronine ( FT 4) decreased gradually P < .001). FibroStage, diagnosis that incorporates data on 4,...
To elucidate the dynamic alterations of metabolites in rat plasma during liver regeneration and search for potential biomarkers regeneration, 65 male Sprague-Dawley rats were divided into three groups: 70% partial hepatectomy group (PHx, n = 30), sham-operated (Sham, pre-PHx (pre-PHx, 5). Rats Sham PHx groups sacrificed after 30 min (min), 6 h (h), 24, 48, 72, 168 surgery (n 5 per time point). The gas chromatography–mass spectrometry-based metabolomic approach was used to identify...
Abstract Aim To determine the prognostic risk factors of patients with hepatitis B virus related acute‐on‐chronic liver failure (HBV‐ACLF) treated plasma exchange (PE)‐based artificial support system (ALSS), and create a predictive model. Methods A total 304 HBV‐ACLF who received PE‐based ALSS were retrospectively analyzed. Potential on admission associated survival investigated. Of note, 101 additional analyzed to validate performance models. Results According 28‐day survival, 207 survived...
We describe a cheap, robust, fast, high-throughput, and flexible proteomic sample processing method based on regular 96-well plate by acetone precipitation under low centrifuge speed (96PACS), which enables predigestion of 96 samples within 2 h. Tested complex Huh-7 total lysate, 96PACS produced comparable proteome coverage even showed better reproducibility than FASP. Quantitative performance was further tested using data-independent acquisition parallel reaction monitoring quantitation in...
Abstract Background COVID-19 continuously threated public health heavily. Present study aimed to investigate the lymphocyte subset alterations with disease severity, imaging manifestation, and delayed hospitalization in patients. Methods Lymphocyte subsets was classified using flow cytometry peripheral blood collected from 106 Results Multivariate logistic regression showed that chest tightness, count, γ-glutamyl transpeptidase were independent predictors for severe COVID-19. The T cell, CD4...
microRNAs, as small endogenous RNAs, influence umpteen sophisticated cellular biological functions regarding neurodegenerative and cerebrovascular diseases. Here, we interrogated miR-381-3p's on BV2 activation neurotoxicity in ischemic hypoxic environment. Oxygen-glucose deprivation (OGD) was adopted to induce microglial HT-22 neuron damage. Quantitative polymerase chain reaction (qRT-PCR) taken check miR-381-3p expression OGD-elicited cells neurons. It transpired that lowered elicited by...
The natural course of coronavirus disease 2019 (COVID-19) patients without clinical intervention has not yet been documented. One hundred and fifty-eight from two hospitals were enrolled to identify the indicators severe COVID-19 observe intervention. total computed tomography (CT) score, a quantitative score based on assessment number, quadrant, area lesions in CT, tended perform better than only number or (p = 0.0004 p 0.0887, respectively). Multivariate logistic regression showed that CT...
Background and Aims: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) remains a serious entity with high mortality. Growth hormone (GH) is related to the metabolism regeneration. The present study aimed explore changes prognostic efficacy of GH on outcome HBV-ACLF. Methods: A prospective cohort 124 patients cross-sectional 142 subjects were enrolled. insulin-like growth factor-1(IGF-1) detected by ELISA. Thirty-day survival was collected association between 30-day...
To observe the stability and sensitivity of transcription mediated amplification (TMA) system, to compare it with real-time reverse polymerase chain reaction (RT-PCR) in amplifying serum HCV RNA infected patients.TMA system was established by moloney murine leukemia virus (MMLV) transcriptase, T7 2 specific primers firstly, then its repeatability were compared at different storage temperatures correlation change curve. The difference between TMA RT-PCR evaluated a group 10-fold diluted...
Acute-on-chronic liver failure (ACLF) and decompensated cirrhosis (DC) are life-threatening syndromes that can develop at the end-stage of chronic hepatitis B virus (HBV) infection. Both ACLF DC complicated by hepatic extrahepatic pathogeneses. To better understand compartment-specific metabolic modulations related to their pathogenesis, HBV-DC, HBV-ACLF patients, controls (30 each) were analyzed metabolomics using portal (Port), vein (Hep), peripheral (Peri) serum. Compartment ratios...
The manifestation of severe pneumonia is only occasional, and pneumomediastinum a condition that occurs rarely in Coronavirus disease 2019 (COVID-19) patients, especially those patients who are infected with the Omicron variant. In addition, whether or often older age, poor physical condition, underlying diseases remains to be ascertained. To date, due infection had not been reported young patient an excellent condition. this study, we report such case aforementioned manifestations robust...
Abstract Aim To weight the prognostic value of thyroid hormones in catastrophic acute‐on‐chronic liver failure (ACLF). Methods A retrospective cohort ( n = 635) and two prospective cohorts 353, 198) were enrolled this study. The performance a novel developed score was assessed from aspects reliability, discrimination, clinical net benefit. Results Thyroid‐stimulating hormone (TSH) identified to have most potential as predictor for hepatitis B virus‐related ACLF among hormones. (modified...