A5027085678- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Viral-associated cancers and disorders
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Ovarian cancer diagnosis and treatment
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Genetic factors in colorectal cancer
- Erythrocyte Function and Pathophysiology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- T-cell and Retrovirus Studies
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Blood properties and coagulation
- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- Digital Imaging for Blood Diseases
- Cancer-related molecular mechanisms research
Aarhus University Hospital
2016-2025
Aarhus University
2016-2025
Universitas Majalengka
2016-2017
Therapy-related myeloid neoplasms (tMN) develop after exposure to cytotoxic and radiation therapy, due their adverse prognosis, it is of paramount interest identify patients at high risk. The presence clonal hematopoiesis has been shown increase the risk developing tMN. value analyzing hematopoietic stem cells harvested leukapheresis before autologous cell transplantation (ASCT) with next-generation sequencing immunophenotyping represents potentially informative parameters that have yet be...
Follicular lymphoma (FL) exhibits considerable variability in biological features and clinical trajectories across patients. To dissect the diversity of FL, we utilized a Bernoulli mixture model to identify genetic subtypes 713 pre-treatment tumor tissue samples. Our analysis revealed existence five with unique profiles that correlated clinicopathological characteristics. The clusters were enriched specific mutations as follows: CS (CREBBP STAT6), TT (TNFAIP3 TP53), GM (GNA13 MEF2B), Q...
Megakaryocytes (MKs) are rare, large, polyploid bone marrow (BM) cells responsible for the production of platelets. The identification and characterization MKs is widely recognized as challenging. Manual microscopy especially difficult due to rarity complex morphology MKs, while flow cytometry faces additional challenges from MKs’ large size, fragility, platelet adhesion, causing false positives. We present a novel approach accurately enrich human BM aspirates with specific focus on...
Despite better therapeutic options and improved survival of diffuse large B-cell lymphoma (DLBCL), 30-40% the patients experience relapse or have primary refractory disease with a dismal prognosis. To identify biological correlates for treatment resistance, we profiled microRNAs (miRNAs) matched relapsed DLBCL by next-generation sequencing. Altogether 492 miRNAs were expressed in samples. Thirteen showed significant differential expression between specimen pairs. Integration differentially...
In the western world, colorectal cancer (CRC) is third most common cause of cancer-related deaths. Survival closely related to stage at diagnosis striking clinical need for biomarkers capable early detection. To search possible biological parameters CRC we evaluated protein expression three CREC (acronym: Cab45, reticulocalbin, ERC-55, calumenin) proteins: calumenin, and ERC-55 in a cellular model consisting normal derived colon mucosa cell line, NCM460, primary adenocarcinoma line colon,...
Interaction of checkpoint receptor programmed death 1 (PD-1) with its ligand (PD-L1) downregulates T cell effector functions and thereby leads to tumor immune escape. Here, we aimed determine the clinical significance soluble PD-1 (sPD-1) PD-L1 (sPD-L1) in patients diffuse large B-cell lymphoma (DLBCL). We included 121 high-risk DLBCL treated Nordic NLG-LBC-05 trial dose-dense immunochemotherapy. sPD-1 sPD-L1 levels were measured from serum samples collected prior treatment, after three...
Oncogenic fusion drivers are common in hematological cancers and thus relevant targets of future CRISPR-Cas9-based treatment strategies. However, breakpoint-location variation patients pose a challenge to traditional breakpoint-targeting CRISPR-Cas9-mediated disruption Here we present new dual intron-targeting CRISPR-Cas9 strategy, for targeting t(8;21) found 5-10% de novo acute myeloid leukemia (AML), which efficiently disrupts genes without prior identification breakpoint location. We show...
Abstract Myelodysplastic syndrome is primarily characterized by dysplasia in the bone marrow (BM), presenting a challenge consistent morphology interpretation. Accurate diagnosis through traditional slide-based analysis difficult, necessitating standardized objective technique. Over past two decades, imaging flow cytometry (IFC) has proven effective combining image-based morphometric analyses with high-parameter phenotyping. We have previously demonstrated effectiveness of IFC feature-based...
Background: Follicular lymphoma (FL) is characterized by an indolent nature and generally favorable prognosis, yet poses a particular clinical challenge, since disease progression observed in notable subset of patients. Currently, it not possible to anticipate which patients will be at risk progression, highlighting the need for reliable predictive biomarkers that can detected early disease. Methods: We applied tandem-mass-tag labelled nano-liquid chromatography tandem mass spectrometry...
We show that the multiligand receptor megalin, known to mediate uptake and trafficking of nutrients signaling molecules, is frequently expressed in malignant melanoma samples. Expression megalin-encoding mRNA was investigated 65 samples nevi, melanomas, metastases observed more than 60% samples, while only 20% benign counterparts. Megalin expression nevus additionally by immunohistochemistry, which confirmed our mRNA-based observations. furthermore a panel tumor-derived cell lines express...
Follicular lymphoma (FL) is an indolent with a generally favorable prognosis. However, histological transformation (HT) to more aggressive disease leads markedly inferior outcomes. This study aims identify biological differences predictive of HT at the time initial FL diagnosis. We show differential protein expression between diagnostic samples from patients subsequent (subsequently-transforming [st-FL]; n = 20) and without (nontransforming [nt-FL]; 34) by label-free quantification nano...
Myeloid and lymphoid malignancies are postulated to have distinct pathogenetic mechanisms. The recent observation that patients with a myeloproliferative neoplasm an increased risk of developing lymphoproliferative malignancy has challenged this assumption. We collected nationwide cohort both malignancies. Patients diagnosed in 1990-2015 were identified through the national Danish Pathology Registry. 599 concomitant or subsequent diagnosis lymphoma. Histopathological review diagnostic...
Radiotherapy is routinely used for management of limited-stage follicular lymphoma (FL), yet half patients ultimately relapse. We hypothesized that the presence specific gene mutations may predict outcomes. performed targeted sequencing a 69-gene panel in 117 FL treated with radiotherapy and identified recurrently mutated genes. CREBBP was most frequently mutated, associated inferior progression-free survival, though not after false discovery rate adjustment. This association failed to...
Objective: Individuals with HIV experience an increased risk of lymphoma, making this important cause death among people HIV. Nevertheless, little is known regarding the underlying genetic aberrations, which we therefore set out to characterize. Design: We conducted next-generation panel sequencing explore mutational status diagnostic lymphoma biopsies from 18 patients diagnosed secondary infection. Methods: Ion Torrent was performed AmpliSeq on HIV-associated B-cell lymphomas ( n = 18),...
Follicular lymphoma (FL) is an indolent, yet generally incurable neoplasia with a median survival exceeding 10 years. However, subset of FL patients experiences histological transformation (HT) to more aggressive lymphoma, in the majority cases diffuse large B-cell (DLBCL). This affects both clinical course and prognostic outcome, resulting markedly reduced after transformation. Thus, early risk stratification prediction at HT would be highly valuable setting. Here, we investigated potential...
Galectin-1 (Gal-1) is known to regulate cell signaling within the immune system and may be a target for new anticancer therapy. In patients with chronic lymphocytic leukemia (CLL) classical Hodgkin lymphoma (cHL), high levels of Gal-1 tumor microenvironment were associated worse disease state or poor outcome. can secreted from cells by an unknown mechanism, in blood samples burden cHL CLL patients. However, serum have never been investigated multiple myeloma (MM). We measured treatment...
Infective endocarditis (IE) has high mortality, partly due to delayed diagnosis. No biomarker can identify IE in patients with fever and clinical picture of infection. To find putative biomarkers we analyzed serum levels two proteins found cardiac valves, fibulin-1 (n=696) osteoprotegerin (n=689) among on suspicion IE. Proteomic analyses were performed 24 bacteremia, 12 definite excluded IE.Fibulin-1 studied by enzyme linked immunosorbent assay (ELISA). conducted 2-dimensional polyacrylamid...
Abstract Background The hallmark of myelodysplastic syndrome (MDS) remains dysplasia in the bone marrow (BM). However, diagnosing MDS may be challenging and subject to inter‐observer variability. Thus, there is an unmet need for novel objective, standardized reproducible methods evaluating dysplasia. Imaging flow cytometry (IFC) offers combined analyses phenotypic image‐based morphometric parameters, example, cell size nuclearity. Hence, we hypothesized IFC a useful tool diagnostics. Methods...