A5035470077- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Multiple Myeloma Research and Treatments
- Digital Imaging for Blood Diseases
- Chronic Myeloid Leukemia Treatments
- Toxoplasma gondii Research Studies
- Immune cells in cancer
- Herpesvirus Infections and Treatments
- Hematopoietic Stem Cell Transplantation
- Hepatitis B Virus Studies
- Erythrocyte Function and Pathophysiology
- T-cell and B-cell Immunology
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- Social and Educational Sciences
- Protein Degradation and Inhibitors
- Hematological disorders and diagnostics
- Blood properties and coagulation
- Dermatology and Skin Diseases
- Mesenchymal stem cell research
- Cancer Genomics and Diagnostics
- SARS-CoV-2 detection and testing
- Blood disorders and treatments
Aarhus University Hospital
2009-2025
Aarhus University
2009-2024
Therapy-related myeloid neoplasms (tMN) develop after exposure to cytotoxic and radiation therapy, due their adverse prognosis, it is of paramount interest identify patients at high risk. The presence clonal hematopoiesis has been shown increase the risk developing tMN. value analyzing hematopoietic stem cells harvested leukapheresis before autologous cell transplantation (ASCT) with next-generation sequencing immunophenotyping represents potentially informative parameters that have yet be...
The aging of the immune system, also named immunosenescence, affects vaccine responses. However, onset age-related immunosenescence has been uncertain, in particularly with regard to Here, we show that formation antibodies response vaccination against hepatitis B virus infection was significantly reduced for donors a mean age 61 y compared group 33 y. Booster sero-converted elderly donors, but only at level, while stronger found young donors. Agreeing these findings, surface antigen-specific...
Megakaryocytes (MKs) are rare, large, polyploid bone marrow (BM) cells responsible for the production of platelets. The identification and characterization MKs is widely recognized as challenging. Manual microscopy especially difficult due to rarity complex morphology MKs, while flow cytometry faces additional challenges from MKs’ large size, fragility, platelet adhesion, causing false positives. We present a novel approach accurately enrich human BM aspirates with specific focus on...
Abstract Myelodysplastic syndrome is primarily characterized by dysplasia in the bone marrow (BM), presenting a challenge consistent morphology interpretation. Accurate diagnosis through traditional slide-based analysis difficult, necessitating standardized objective technique. Over past two decades, imaging flow cytometry (IFC) has proven effective combining image-based morphometric analyses with high-parameter phenotyping. We have previously demonstrated effectiveness of IFC feature-based...
Acute myeloid leukemia (AML) is a heterogenous blood cancer with dismal prognosis. It emanates from leukemic stem cells (LSCs) arising the genetic transformation of hematopoietic (HSCs). LSCs hold prognostic value, but their molecular and immunophenotypic heterogeneity poses challenges: there no single marker for identifying all across AML samples. We hypothesized that imaging flow cytometry (IFC) paired artificial intelligence-driven image analysis could visually distinguish HSCs based...
Abstract Background The hallmark of myelodysplastic syndrome (MDS) remains dysplasia in the bone marrow (BM). However, diagnosing MDS may be challenging and subject to inter‐observer variability. Thus, there is an unmet need for novel objective, standardized reproducible methods evaluating dysplasia. Imaging flow cytometry (IFC) offers combined analyses phenotypic image‐based morphometric parameters, example, cell size nuclearity. Hence, we hypothesized IFC a useful tool diagnostics. Methods...
Abstract In adult acute myeloid leukaemia (AML), immunophenotypic differences enable discrimination of leukaemic stem cells (LSCs) from healthy haematopoietic (HSCs). However, cell characteristics are less explored in paediatric AML. Employing a 15‐colour flow cytometry assay, we analysed the expression eight aberrant surface markers together with BCL‐2 on CD34 + CD38 − bone marrow 38 AML patients and seven non‐leukaemic, age‐matched controls. Furthermore, clonality was investigated by...
Novel therapeutic tools are warranted to improve outcomes for children with acute myeloid leukemia (AML). Differences in the proteome of leukemic blasts and stem cells (AML-SCs) AML compared normal hematopoietic (HSCs) may facilitate identification potential targets future treatment strategies. In this explorative study, we used mass spectrometry compare AML-SCs CLEC12A+ from five pediatric patients HSCs progenitor hematologically healthy, age-matched controls. A total 456 shared proteins...
Protein antigens comprising peptide motifs with high binding affinity to major histocompatibility complex class I (MHC-I) molecules are expected induce a stronger cytotoxic T-lymphocyte response and thus provide better protection against infection microorganisms where T-cells the main effector arm of immune system.Data on cyst formation survival were extracted from past studies DNA immunization mice plasmids coding for Toxoplasma gondii antigens. From in silico analyses vaccine antigens,...
Abstract This panel was designed to identify, quantify and phenotypically characterize putative leukemic stem cells (LSCs) in bone marrow (BM) samples from individual pediatric patients diagnosed with acute myeloid leukemia (AML). Based on an aberrant expression immunophenotypically defined hematopoietic (HSCs), several antigens have been proposed as LSC markers AML research, using healthy adult BM reference material. Generally, these evaluated individually smaller panels (e.g. 8‐color...
Abstract Background Studies in T‐cell acute lymphoblastic leukemia (T‐ALL) have shown that leukemic blast populations may display immunophenotypic heterogeneity. In the clinical setting, evaluation of measurable residual disease during treatment and follow‐up is highly dependent on knowledge diversity subsets. Here, we set out to evaluate whether variation expression marker, TdT, T‐ALL blasts could correspond differences morphometric features. Methods We investigated diagnostic bone marrow...
Abstract
 
 Background
 Psoriasis is an inflammatory disease characterized by leukocyte skin infiltration. Interestingly, recent works suggest that the migration of dendritic cells (DCs) abnormal in psoriatic skin. DCs have significant role regulating function T lymphocytes, at least part influenced local environment cytokines. In lesions expression IL-20 highly up-regulated. It unclear if this cytokine has any influence on DCs.
 Methods
 Here, we investigated...