A5101464417- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Renal and related cancers
- Cystic Fibrosis Research Advances
- Virus-based gene therapy research
- Ovarian function and disorders
- Xenotransplantation and immune response
- Neonatal Respiratory Health Research
- Sperm and Testicular Function
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bioenergy crop production and management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Biomedical Ethics and Regulation
- Prenatal Screening and Diagnostics
- Energy Harvesting in Wireless Networks
- Wound Healing and Treatments
- Viral Infections and Immunology Research
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
Utah State University
2014-2024
Guangdong Medical College
2019
Third Xiangya Hospital
2018
Central South University
2018
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. The major cause of limited life span patients progressive lung disease. models have been generated 4 species (mice, rats, ferrets, and pigs) to enhance our understanding pathogenesis. Sheep may be particularly relevant animal model humans due similarities anatomy development two species. Here, we describe generation sheep for using CRISPR/Cas9 genome editing somatic cell...
Significance Transfer of sperm-producing stem cells isolated from a donor male into testes recipient has important applications for preserving germplasm and as conduit widespread dissemination desirable genetics in livestock production. A key aspect is surrogate males that lack endogenous germline but are otherwise physiologically normal. Here we demonstrate mice, pigs, goats rendered genetically sterile by CRISPR-Cas9 editing the NANOS2 gene support donor-derived spermatogenesis following...
The golden Syrian hamster is the model of choice or only rodent for studying many human diseases. However, lack gene targeting tools in hamsters severely limits their use biomedical research. Here, we report first successful application CRISPR/Cas9 system to efficiently conduct hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three coding sequences different functional domains STAT2 protein, one KCNQ1, and PPP1R12C—and demonstrated that highly efficient introducing...
Abstract Pharmacological inhibition of reactive oxygen species (ROS) is a potential strategy to prevent diabetes-induced cardiac dysfunction. This study was designed investigate precise effects antioxidant N ‑acetylcysteine (NAC) in alleviating diabetic cardiomyopathy (DCM). Echocardiography and histologic studies were performed 12 weeks after streptozocin injection. Protein levels involved endoplasmic reticulum stress (ERS) apoptosis analyzed by western blotting hearts or high-glucose (HG,...
Pluripotent stem cells (PSCs) have been successfully developed in many species. However, the establishment of bovine-induced pluripotent (biPSCs) has challenging. Here we report generation biPSCs from bovine mesenchymal (bMSCs) by overexpression lysine-specific demethylase 4A (KDM4A) and other reprogramming factors OCT4, SOX2, KLF4, cMYC, LIN28, NANOG (KdOSKMLN). These exhibited silenced transgene expression at passage 10, had prolonged self-renewal capacity for over 70 passages. The flat,...
The pronuclear (PN) injection technique was first established in mice to introduce foreign genetic materials into the pronuclei of one-cell stage embryos. introduced material may integrate embryonic genome and generate transgenic animals with information following transfer injected embryos foster mothers. Following success mice, PN has been applied successfully many other animal species. Recently, employed reagents gene-modifying activities, such as CRISPR/Cas9 system, achieve site-specific...
Animal models that are susceptible to SARS-CoV-2 infection and develop clinical signs like human COVID-19 desired understand viral pathogenesis effective medical countermeasures. The golden Syrian hamster is important for the study of since hamsters naturally SARS-CoV-2. However, infected show only limited disease resolve quickly. In this study, we describe development angiotensin-converting enzyme 2 (hACE2) transgenic as a model COVID-19. During SARS-CoV-2, observed different hACE2 founder...
Cystic Fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. The F508del and G542X are most common found US patients, accounting for 86.4% 4.6% of all mutations, respectively. causes deletion phenylalanine residue at position 508 associated with impaired CFTR protein folding. nonsense mutation that introduces stop codon into mRNA, thus preventing normal synthesis. Here, we describe generation CFTRF508del/F508del CFTRG542X/G542X...
Abstract To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein development of a Tc goat system expressing in their sera. In system, artificial chromosome (HAC) comprising entire immunoglobulin (Ig) gene repertoire germline configuration was introduced into genetic makeup domestic goat. We achieved this by transferring HAC fetal fibroblast cells...
Type I interferons (IFNs) initiate immune responses to viral infections. Their effects are mediated by the type IFN receptor, IFNAR, comprised of two subunits: IFNAR1 and IFNAR2. One or both chains sheep IFNAR were disrupted in fetal fibroblast lines using CRISPR/Cas9 12 lambs produced somatic cell nuclear transfer (SCNT). Quantitative reverse transcription-polymerase chain reaction for IFN-stimulated gene expression showed that deficient fail respond IFN-alpha. Furthermore, cells from an...
Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of precious transgenic lines or resetting lifespan primary cells multiple genetic modifications. We successfully produced second-generation cloned goats using donor neonatal fibroblasts from first-generation clones. However, our attempts to produce any third-generation clones failed. SCNT efficiency decreased progressively with clonal generations. The rate pregnancy loss was significantly greater in...
Highly effective modulator therapies for cystic fibrosis (CF) make it a treatable condition many people. However, although CF respiratory illness occurs after birth, other organ systems particularly in the digestive tract are damaged before birth. We use an ovine model of to investigate utero origins disease since sheep closely mirrors critical aspects human development. Wildtype (WT) and
Reprogramming of the gamete into a developmentally competent embryo identity is fundamental aspect preimplantation development. One most important processes this reprogramming transcriptional awakening during embryonic genome activation (EGA), which robustly occurs in fertilized embryos but defective somatic cell nuclear transfer (SCNT) embryos. However, little known about genome-wide underlying chromatin landscape EGA SCNT and how it differs from embryo. By profiling open both types bovine...
Microchimerism is defined as the presence of a small population cells or DNA in 1 organism originated from genetically different organism. It well established that this phenomenon occurs humans and mice are exchanged between mother fetus during gestation. Currently, no information available about maternal microchimerism goats, only published study limited to an evaluation fetal fetal-fetal blood samples following natural breeding. In order determine whether bidirectional fetal-maternal cell...
The Tibetan Argali (Ovis ammon hodgsoni), a wild sheep of the subfamily Caprinae (Bovidae), primarily found in Plateau, is categorized as near threatened on International Union for Conservation Nature (IUCN) Red List. For conservation this species, we have achieved cloning full-term live lamb (died shortly after birth) from male cell line that has been cryopreserved more than 20 years. While working towards goal Argali, also recognised need to establish reproductive herd animals by producing...
Golden Syrian hamster embryos are difficult to cryopreserve due their high sensitivity cryoprotectants and in vitro handling. The objective of this study is develop a robust open pulled straw (OPS) vitrification technique for cryopreserving at various developmental stages. We first systematically tested the concentrations exposure times two-cell solutions. identified pretreatment with 10% (v/v) ethylene glycol (EG) + dimethylsulfoxide (DMSO) 30 s followed by solution, EDFS30 (containing 15%...
The yield of blastocysts and hatched using in vitro production (IVP) goats are still low. development caprine embryos is frequently arrested at the 8- to 16-cell stage, indicating suboptimal culture conditions (Jimenez-Macedo et al. 2005 Theriogenology 64, 1249–1262). Our goal was produce transgenic by somatic cell nuclear transfer (SCNT) further determine whether length embryo has an effect on term. We compared efficiency transferring single-cell 12 h post-activation 4- 8-cell cultured for...
The pronuclear (PN) injection technique was first established in mice to introduce foreign genetic materials into the pronuclei of one-cell stage embryos. introduced material may integrate embryonic genome and generate transgenic animals with information following transfer injected embryos foster mothers. Following success mice, PN has been applied successfully many other animal species. Recently, employed reagents gene-modifying activities, such as CRISPR/Cas9 system, achieve site-specific...