A5100746803- Tuberculosis Research and Epidemiology
- Genetic diversity and population structure
- Mycobacterium research and diagnosis
- Aquaculture disease management and microbiota
- Genetic and phenotypic traits in livestock
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Reproductive Biology and Fertility
- interferon and immune responses
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Identification and Quantification in Food
- Peroxisome Proliferator-Activated Receptors
- Vitamin C and Antioxidants Research
- HIV/AIDS Research and Interventions
- Renal and related cancers
- RNA and protein synthesis mechanisms
- Antioxidant Activity and Oxidative Stress
- Animal Virus Infections Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Fish Biology and Ecology Studies
Shihezi University
2019-2025
Huazhong University of Science and Technology
2020-2024
Tongji Hospital
2023-2024
Wuhan National Laboratory for Optoelectronics
2022-2024
Guangxi Medical University
2024
First Affiliated Hospital of GuangXi Medical University
2024
South China Agricultural University
2018-2023
Huazhong Agricultural University
2010-2022
Kunming University
2022
Yunnan Center for Disease Control And Prevention
2019-2022
The CRISPR/Cas9 system has been adapted as an efficient genome editing tool in laboratory animals such mice, rats, zebrafish and pigs. Here, we report that mediated approach can efficiently induce monoallelic biallelic gene knockout goat primary fibroblasts. Four genes were disrupted simultaneously fibroblasts by CRISPR/Cas9-mediated editing. single-gene successfully used for somatic cell nuclear transfer (SCNT) resulted live-born goats harboring mutations. represents a highly effective...
The Tibetan cashmere goat (Capra hircus), one of the most ancient breeds in China, has historically been a critical source meat and production for local farmers. To adapt to high-altitude area, extremely harsh climate, hypoxic environment that lives in, this developed distinct phenotypic traits compared lowland breeds. However, genetic components underlying adaptation remain largely unknown.
Tuberculosis (TB) is a global health threat, killing approximately 1.5 million people each year. The eradication of Mycobacterium tuberculosis, the main causative agent TB, increasingly challenging due to emergence extensive drug-resistant strains. Vaccination considered an effective way protect host from pathogens, but only clinically approved TB vaccine, Bacillus Calmette-Guérin (BCG), has limited protection in adults. Multi-epitope vaccines have been found enhance immunity diseases by...
Abstract Background Biodiesel, an emerging sustainable and renewable clean energy, has garnered considerable attention as alternative to fossil fuels. Although lipases are promising catalysts for biodiesel production, their efficiency in industrial-scale application still requires improvement. Results In this study, a novel strategy multi-site mutagenesis the binding pocket was developed via FuncLib (for mutant enzyme design) Rosetta Cartesian_ddg free energy calculation) improve reaction...
Freshwater resources are scarce in arid regions, and the rational use of brackish water can alleviate local freshwater shortages, but long-term drip irrigation increases risk soil salinization, which turn affects nutrient transformation microbial diversity. Soil phosphorus availability is critical for crop growth, yet it unclear how will affect transformation. Therefore, to investigate effects on phosphorus-transforming microorganisms their functional genes cotton fields, experiment was set...
Analysis of the protein-protein interaction network a pathogen is powerful approach for dissecting gene function, potential signal transduction, and virulence pathways. This study looks at construction global (PPI) human Mycobacterium tuberculosis H37Rv, based on high-throughput bacterial two-hybrid method. Almost entire ORFeome was cloned, more than 8000 novel interactions were identified. The overall quality PPI validated through two independent methods, high success rate 60% obtained....
Rhizopus oryzae lipase (ROL) is very attractive in biotechnology and industry as a safe environmentally friendly biocatalyst. Functional expression of ROL Escherichia coli facilitates effective high-throughput screening for positive variants.
RelBE represents a typical bacterial toxin-antitoxin (TA) system. Mycobacterium tuberculosis H37Rv, the pathogen responsible for human tuberculosis, contains three RelBE-like modules, RelBE, RelFG, and RelJK, which are at least partly expressed in macrophages during infection. modules appear to be autoregulated an atypical manner compared other TA systems; however, molecular mechanisms potential interactions between different remain elucidated. In present study, we characterized interaction...
Transcriptional regulation plays a critical role in the life cycle of Mycobacterium smegmatis and its related species, M. tuberculosis, causative microbe for tuberculosis. However, key transcriptional factors involved broad diverse genes remain to be characterized mycobacteria. In present study, TetR-like family factor, Ms6564, was as master regulator. A conserved 19 bp-palindromic motif identified Ms6564 binding using DNaseI footprinting EMSA. total 339 potential target were further by...
Viral nervous necrosis (VNN) is an acute and serious fish disease caused by virus (NNV) which has been reported massive mortality in more than fifty teleost species worldwide. VNN causes damage of vacuolation to central system (CNS) cells fish. It difficult identify the specific type cell targeted NNV, decipher host immune response because functional diversity highly complex anatomical cellular composition CNS. In this study, we found that red spotted grouper NNV (RGNNV) mainly attacked...
The genome of Mycobacterium tuberculosis, the causative agent encodes a large number putative transcriptional regulators. However, identity and target genes only few them have been clearly identified to date. In recent study, ArsR family regulator Rv2034 was characterized as novel positive phoP. current we auto-repressive capabilities several residues in protein critical for its DNA binding activities. We also provide evidence that forms dimers vitro. Furthermore, by using DNaseI...
Isoniazid (INH) and ethambutol (EMB) are two major first-line drugs for managing tuberculosis (TB), caused by the microbe Mycobacterium Although co-use of these is common in clinical practice, mechanism potential synergistic interplay between them remains unclear. Here, we present first evidence that INH EMB act synergistically through a transcriptional repressor inhA gene, target gene encoding an enoyl-acyl carrier protein reductase fatty acid synthase type II system required bacterial cell...
Camellia is a genus of flowering plants in the family Theaceae, and several species this have economic importance. Although great deal molecular makers has been developed for assisted breeding past decade, number simple sequence repeats (SSRs) publicly available insufficient. In study, total 28,854 potential SSRs were identified with frequency 4.63 kb. A 172 primer pairs synthesized preliminarily screened 10 C. japonica accessions, these pairs, 111 found to be polymorphic. Fifty-one...
Beige fat activation involves a fuel switch to fatty acid oxidation following chronic cold adaptation. Mitochondrial acyl-CoA synthetase long-chain family member 1 (ACSL1) localizes in the mitochondria and plays key role oxidation; however, regulatory mechanism of subcellular localization remains poorly understood. Here, we identify an endosomal trafficking component sortilin (encoded by Sort1) adipose tissues that shows dynamic expression during beige facilitates translocation ACSL1 from...
Growth hormone (GH) has been considered as a candidate gene for growth traits in fish. In this study, polymorphisms of the GH were evaluated associations with 282 Siniperca chuatsi individuals. Using directly sequencing, four single nucleotide (SNPs) identified gene, two mutations intron 4 (g.4940A>C, g.4948A>T), one mutation exon 5 (g.5045T>C) and (g.5234T>G). Notably, three them significantly associated performance, particularly g.4940A>C which was highly correlated all...
Nicosulfuron is an ingredient in photosynthesis-inhibiting herbicides and has been widely used corn post-emergence weed control. In the current study, a pair of sister lines, HK301 (nicosulfuron-tolerence, NT) HK320 (nicosulfuron-sensitive, NS), was to study effect nicosulfuron sweet maize seedlings on C4 photosynthetic enzymes non-enzymatic substances, expression levels key enzymes, chloroplast structure. sprayed at four-leaf stage, water as After treatment, phosphoenolpyruvate carboxylase...
In nature, barnacles and bacterial biofilms utilize self-assembly amyloid to achieve strong robust interface adhesion. However, there is still a lack of sufficient research on the construction macroscopic adhesives based amyloid-like nanostructures through reasonable molecular design. Here, we report genetically programmed living-cell bioadhesive inspired by barnacle curli system. Firstly, encoding genes two natural adhesion proteins (CsgA cp19k) derived from E. coli cement were fused...
HIV drug resistance increased with the widespread use of antiretroviral drugs, and posed great threat to therapy (ART). Pu'er Prefecture, lying in southwest Yunnan Province, China, borders Myanmar, Laos Vietnam, is also area where AIDS was discovered earlier, however, which there has been no information on resistance.A cross-sectional survey pretreatment (PDR) conducted Prefecture 2021. Partial pol gene sequences were obtained analyze construct genetic transmission network. analyzed using...
T-box transcription factor T (TBXT), encoding the brachyury protein, is an embryonic nuclear involved in mesoderm formation and differentiation. Previous studies indicate that TBXT mutations are responsible for tailless or short-tailed phenotype of many vertebrates. To verify whether fat-rumped sheep associated with mutations, exon 2 gene 301 individuals belonging to 13 Chinese Iranian breeds was directly sequenced. Meanwhile, 380 samples were used detect genotypes candidate variations by...