The molecular and population genetic evidence of the phylogenetic status Tibetan sheep (Ovis aries) is not well understood, little known about this species' diversity. This knowledge gap partly due to difficulty sample collection. first work address question. Here, diversity relationship 636 individual from fifteen populations were assessed using 642 complete sequences mitochondrial DNA D-loop. Samples collected Qinghai-Tibetan Plateau area in China, reference data obtained six breed...

Fat tail is a unique trait in sheep acquired during domestication. Several genomic analyses have been conducted breeds from limited geographic origins to identify the genetic factors underlying this trait. Nevertheless, these studies obtained different candidates. The results of regional were easily biased by breed structures.To minimize bias and distinguish true candidates, we used an extended data set 968 representing 18 fat-tailed 14 thin-tailed around world, integrated two statistical...

Summary DNA sequence variation has been widely reported as the genetic basis for adaptation, in both humans and other animals, to hypoxic environment experienced at high altitudes. However, little is known about patterns of gene expression underlying such adaptations. In this study, we examined differences transcriptomes four organs (heart, kidney, liver lung) between yak cattle, a pair closely related species distributed low altitudes respectively. Of examined, heart shows greatest...

Tibetan sheep, an indigenous breed, have a wide variety of phenotypes and colorful coat, which make this breed interesting model for evaluating the effects coat-color gene mutations on phenotypic trait. The agouti signaling protein (ASIP) is positional candidate gene, as was inferred based previous study. In our research, ASIP copy numbers in genomic DNA were detected using novel approach, exon 2 g.100-104 mutation number variation (CNV) associated with coat color 256 sheep collected from...

Cashmere goats, as one of the important domesticated animal species, are known for their high-quality fiber. The growth cashmere has seasonal variations caused by photoperiodic changes, but molecular genetic mechanisms underlying this phenotype including functional role long non-coding RNAs (lncRNA) is still poorly understood. In study, we analyzed RNA-seq dataset 39 goat skin samples all different stages and identified 1591 lncRNAs. These lncRNAs exhibited stage-specific expression...

Deleterious mutations play an important functional role, affecting trait phenotypes in ways that decrease the fitness of organisms. Estimating frequency occurrence and abundance has been a topic much interest, especially crops livestock. The processes domestication breeding allow deleterious to persist at high frequency, identifying such is particularly for breed improvement. Here, we assessed genome-wide patterns variation 59 domestic 13 wild yaks using genome resequencing data. Based on...

T-box transcription factor T (TBXT), encoding the brachyury protein, is an embryonic nuclear involved in mesoderm formation and differentiation. Previous studies indicate that TBXT mutations are responsible for tailless or short-tailed phenotype of many vertebrates. To verify whether fat-rumped sheep associated with mutations, exon 2 gene 301 individuals belonging to 13 Chinese Iranian breeds was directly sequenced. Meanwhile, 380 samples were used detect genotypes candidate variations by...

Abstract Bone morphogenetic proteins ( BMP s) and the growth factors GDF play an important role in ovarian folliculogenesis essential regulator of processes numerous granulosa cells. 15 gene variations linked to various phenotypic consequences subject species, from infertility improved prolificacy sheep, primary insufficiency women or associated with minor subfertility mouse. To study evolving 9, a phylogenetic analysis was performed. find out candidate mammals, nucleotide sequence 9 genes...

Bone morphogenetic protein 2 (BMP2) is strongly selected in both fat-tailed and thin-tailed sheep may be a candidate gene for tail type selection. However, the mechanism of action BMP2 fat deposition remains unclear. This study investigated genetic variation haplotype combinations with different types, aiming to reveal molecular deposition. We detected total three single nucleotide polymorphisms (SNPs) (g.48401619 T > A, g.48401272 C g.48401136 T) among 533 sheep. The alleles genotype...

Tail adipose as one of the important functional tissues can enhance hazardous environments tolerance for sheep. The objective this study was to gain insight into underlying development mechanisms trait. A quantitative analysis protein abundance in ovine tail/rump tissue performed between Chinese local fat- (Kazakh, Hu and Lanzhou) thin-tailed (Alpine Merino, Tibetan) sheep present by using lable-free approach. Results showed that 3400 proteins were identified five breeds, 804 differentially...

The Yili goose is the only indigenous breed that originates from Anser anser in China, known for its adaptability, strong flying ability, and tender meat with a low body lipid content. liver plays crucial role glucose metabolism, including intake, secretion, transportation, storage of fatty acids (FAs). In this study, RNA-sequencing (RNA-seq) technology was performed to analyze differentially expressed genes geese their hybrid investigate differences metabolism. A total 452 (Q-value < 0.05)...

The desmoglein 4 (DSG4) gene is a potential candidate in the search for genes that may affect wool traits, because of its function.This study aimed to screen polymorphisms partial exon 16 and 3ꞌUTR sheep DSG4 gene, test possible association with length crimp associated fur.Overall, 326 were scanned via single-strand conformational polymorphism assay, through three pairs primers.The breeds included Tan, Han, TanxHan from China, Polled Dorset Australia, Suffolk Britain genotypes AA, BB, AB...

Summary The genetic consequences of population differentiation and isolation have been the subject conservation biology. In this study, we analysed diversity structure Mongolian sheep in China. These animals belong to a traditional local breed with high production, extensive adaption, early maturity roughage resistance. For purpose, 26 microsatellites were genotyped for five populations. Bayesian clustering indicated clusters as most probable populations investigated. addition, clear was...

Wool is produced via synthetic processes of wool follicles, which are embedded in the skin sheep.The development new-generation sequencing and RNA provides new approaches that may elucidate molecular regulation mechanism follicle facilitate enhanced selection for traits through gene-assisted or targeted gene manipulation.We performed de novo transcriptome using Illumina Hiseq 2000 system sheep (Ovis aries).Transcriptome assembly was carried out short-read programs, including SOAPdenovo...

Coat color is a key economic trait in sheep. Some candidate genes associated with animal's coat were found. Particularly, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) and microphthalmia-associated transcription factor (MITF) play role the modulation of hair pigmentation mammals. This study investigated those two genes' mutations expressions wool Tibetan First, gene polymorphisms analyzed, then, relative mRNA expression levels skin tissue different colors compared....

Cashmere goats possess two types of hair follicles, with the secondary follicles producing valuable cashmere fiber used for textiles. The growth exhibits a seasonal pattern arising from photoperiod change. Transcription factors play crucial roles during this process. transcription factor, cold-shock domain, containing C2 (Csdc2) plays role in modulating cell proliferation and differentiation. Our preceding research indicated that expression Csdc2 changes periodically anagen to telogen....

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype.Non systems are therefore important increase simplicity improve turn-around without compromising assay sensitivity accuracy.In this report, we assessed latest non-gelbased methods, high-resolution melting (HRM) curve analysis.HRM is a technique monitors exactly decreasing fluorescence intercalating dye...

Kazakh sheep are seasonal estrous animals, and gonadotropin-releasing hormone (GnRH) is the key to fertility regulation. The nutritional level has a certain regulatory effect on estrous, vitamin B folate plays role in DNA methylation, directly participating process. goal of this study was determine whether involved GnAQ methylation its GnRH secretion. hypothalamic neurons fetal were treated with at concentrations 0 mg/mL, 4 40 80 mg/mL. promoter DNMT1, expression, secretion following...

This research was aimed to identify the changes of nucleotide (Single Nucleotide Polymorphism) growth hormone gene in population Aceh cattle. There were 44 samples DNA sequenced, and a few from Gen Bank (M57764). Based on analysis using MEGA program, it identified one new mutation exon five 2230 bp which C turned into T nucleotide, this called Silent Mutation (Leusine-Leusine/ CTC-CTT). The frequency Single Polymorphism (SNP) genotype (C/T) CC (0.36), TT (0.14) CT (0.50). not possessed by...

The wild Huoba Tibetan sheep belongs to the subfamily Caprinae, which distributes in Town of Tibet Autonomous Region, China. In present work, we report complete mitochondrial genome sequence for first time. total length mitogenome is 16 621 bp, consisting 13 protein-coding genes, 22 transfer RNA (tRNA) two ribosomal (rRNA) and a non-coding control region (D-loop region). As other mammals, most genes are encoded on heavy strand. Its overall base composition A: 33.64%, T: 27.32%, C: 25.90%, G:...

Table S1 Distribution of the Y-chromosomal haplotypes in 24 sheep breeds. As a service to our authors and readers, this journal provides supporting information supplied by authors. Such materials are peer-reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical support issues arising from (other than missing files) should addressed Please note: The publisher is responsible content functionality any Any queries content) directed corresponding author article.