A5038589189- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Muscle Physiology and Disorders
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Nuclear Structure and Function
- Congenital Anomalies and Fetal Surgery
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Meat and Animal Product Quality
- RNA Research and Splicing
- Growth Hormone and Insulin-like Growth Factors
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Ruminant Nutrition and Digestive Physiology
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Regulation of Appetite and Obesity
- Selenium in Biological Systems
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuroendocrine regulation and behavior
Anhui Agricultural University
2016-2025
Poultry Research Institute
2024
Endo Pharmaceuticals (United States)
2024
Fuyang Normal University
2023
Newcastle University
2019-2020
Chinese Academy of Agricultural Sciences
2010-2011
University of Pittsburgh
2005-2011
Xinjiang University
2010
Inner Mongolia Agricultural University
2010
Institute of Electrical and Electronics Engineers
2006
Previous studies have confirmed that methylation regulates gene transcription in the hypothalamus-pituitary-gonadal axis during puberty initiation, but little is known about regulation of DNA on expression pineal gland. To screen gland candidate genes related to onset goat and regulated by genome methylation, we collected glands from prepubertal pubertal female goats, then, determined profile whole bisulfite sequencing transcriptome RNA Illumina HiSeqTM2500. We analyzed differentially...
Abstract Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role reproductive axis male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed arcuate nucleus (ARC), paraventricular (PVN), periventricular (PeN), and lateral hypothalamic area (LHA) hypothalamus; adenohypophysis Leydig cells rats. Moreover, concentrations serum mRNA hypothalamo-pituitary-gonadal (HPGA) vary with age rat. After intracerebroventricular injection...
Ovarian follicular development and hormone secretion are complex coordinated biological processes which will usually be altered during pregnancy. function is tightly regulated by a multitude of genes, also some specific miRNAs. It necessary to identify the differentially expressed miRNAs in ovaries pregnant non-pregnant mammals, order further understand role miRNA-mediated post-transcriptional regulation mammalian reproduction. Here, we performed comprehensive search for hircine using two...
Superior kidding rate is an important economic trait in production of meat goat, and ovulation the precondition rate. MicroRNAs (miRNAs) play critical roles almost all ovarian biological processes, including folliculogenesis, follicle development, atresia, luteal development regression. To find out different activity recruitment with miRNA-mediated posttranscriptional regulation, small RNAs expressed pattern tissues multiple uniparous Anhui White goats during follicular phase was analyzed...
Asian corn borer (ACB), Ostrinia furnacalis (Guenée), is the major insect pest of maize in China and countries East Southeast Asia, Pacific Australasia. ACB can develop strong resistance to transgenic Bt expressing Cry1Ab, most widely commercialized worldwide. However, molecular basis for mechanisms Cry1Ab remained unclear. Two biological replicates transcriptome susceptible (ACB-BtS) resistant (ACB-AbR) strains were sequenced using Solexa/Illumina RNA-Seq technology identify...
Long noncoding RNAs (lncRNAs) are involved in regulating animal development, however, their function the onset of puberty goats remain largely unexplored. To identify genes controlling regulation goats, we measured lncRNA and mRNA expression levels from hypothalamus. We applied RNA sequencing analysis to examine hypothalamus pubertal (case; n = 3) prepubertal (control; goats. Our results showed 2943 predicted lncRNAs, including 2012 differentially expressed which corresponded 5412 target...
The low full-term developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos is mainly attributed to imperfect epigenetic reprogramming in the early embryos. However, dynamic expression patterns histone methylation involved progression during SCNT embryo development remain be unknown. In this study, we characterized and compared multiple markers including transcriptionally repressive (H3K9me2, H3K9me3, H3K27me2, H3K27me3, H4K20me2 H4K20me3) active modifications...
Female pubertal development is tightly controlled by complex mechanisms, including neuroendocrine and epigenetic regulatory pathways. Specific gene expression patterns can be influenced DNA methylation changes in the hypothalamus, which turn regulate timing of puberty onset. In order to understand relationship between hypothalamus goats, whole-genome bisulfite sequencing RNA-sequencing analyses were carried out. There was a decline levels during 268 differentially methylated regions (DMR)...
This study found that miR-27 is expressed in muscle and regulates proliferation differentiation. We explored the function regulatory mechanism of miR-27b goat Compared with Boer goat, higher expression was observed all collected tissues Anhuai excluding kidney, whereas opposite pattern for Pax3, which showed lower goat. Expression decreased gradually during skeletal satellite cells increased differentiation; however, Pax3 opposite. The activity demonstrated inhibited cells, but promoted...
A series of complex processes regulate muscle development, and lncRNAs play essential roles in the regulation skeletal myogenesis. Using RNA sequencing, we profiled lncRNA expression during goat (Capra hircus) which included seven stages across fetal 45 (F45), 65 (F65), 90 (F90), 120 (F120), 135 (F135) days, born for 24 h (B1) (B90) days. total 15,079 were identified stages, they less conservative with other species (human, cow, mouse). Among them, 547 differentially expressed, divided into...
Abstract Background Circular RNA (circRNA) is produced during the splicing of mRNA (in addition to linear splicing) and part gene regulatory network. The temporal expression patterns different developmental stages were inseparable from these molecules’ function. Results Skeletal muscles Anhui white goat (AWG) across seven fetal postnatal development sequenced 21 sequencing libraries constructed. We thereby identified 9090 circRNAs analyzed their molecular properties, patterns, potential...
After sexual maturity, male goats will exhibit fighting and mounting behaviors due to the increase of testosterone level, resulting in slower growth rate mutton odor. However, traditional castration methods cause significant stress. In contrast, immunocastration is a welfare-friendly gentler approach replace mechanical castration. Therefore, it important develop vaccines with good immune effects. GnRH octamer vaccine (G8), LHR tandem (LG) were designed prepared for this study. Forty...
Y chromosome acts as a single nonrecombining unit that is male specific and in effect haploid, thus ensuring the preservation of mutational events haplotype via lines. In this study, 6 chromosome–specific microsatellites (SSR) were tested for patrilineal genetic variations 573 samples from Chinese domestic horse (30 breeds), Przewalski's horse, donkey. All loci appeared block There notable differences, however, at markers between 2 haplotypes Eca.YA16 breeds, Haplotype A (Allele A: 156 bp) B...
Abstract The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), can develop strong resistance to Cry1Ab, the most widely commercialized Cry toxin for Bt maize worldwide. It is essential understand mechanism of management this species, but information on post-transcriptional regulation in target insect limited. In present study, RNA was extracted from ACB various larval stages (1–5 instar) Cry1Ab-sensitive (ACB-BtS) and -resistant (ACB-AbR) strains, each which included two biological...
Age at puberty is an important factor affecting goat fertility, with endocrine and genetic factors playing a crucial role in the onset of puberty. To better understand relationship between mechanisms underlying goats, reproductive hormone levels were analyzed by ELISA ultraperformance liquid chromatography-multiple reaction monitoring-multistage/mass spectrometry RNA sequencing was performed to analyze ovarian genes. Serum follicle stimulating hormone, luteinizing estradiol,...
Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of antioxidant system and causes lipid oxidative damage, destroying its structure function. Curcumin is natural plant extract neutralizes enhances function endogenous enzymes. The effect curcumin on preservation sheep has not been reported. This study aims determine effects refrigerated (4 °C) analyze metabolism from Chinese...
Long non-coding RNAs (lncRNAs) play important roles in almost all biological processes. However, there is little information on the effects of lncRNAs ovulation and lambing rates. In present study, we used high-throughput RNA sequencing to identify differentially expressed between ovaries multiparous (Mul) uniparous (Uni) Anhui White goats. Among 107,255,422 clean reads, 183,754 were significantly Uni Mul. them, 455 co-expressed two samples, whereas, 157,523 uniquely Uni, 25,776 Through Cis...
The goat is an important farm animal. Reproduction process of farming. ovary the most reproductive organ for goats. In recent years, increasing number long non-coding RNAs (lncRNAs) have been implicated in regulation mammal reproduction. However, there are few studies on function lncRNAs reproduction, particularly ovary.The sequencing ovaries generated 1,122,014,112 clean reads, and 4926 1454 TUCPs (transcripts uncertain coding potential) were identified further analysis by using potential...
Mutations in the myostatin (MSTN) gene can inactivate its expression and result a non-functional protein, which leads to dramatic muscularity "double-muscling" phenomenon many species.Using sequencing polymerase chain reaction-singlestrand conformation polymorphism methods, polymorphisms of MSTN were investigated as candidate marker for growth 288 goats.The results showed 2 novel single nucleotide polymorphisms: DQ167575 g.197G>A 345A>T.Three potential genotypes (AA, AB, BB) substitution...
Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little known about function lncRNAs (long noncoding RNAs) puberty. Therefore, RNA-seq analysis were performed between goats rats to clarify roles mRNAs In present study, length lncRNAs, open reading frame exon count compared two species. Furthermore, functional annotation based on Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) target genes differentially expressed...
Pre-implantation development is a dynamic, complex and precisely regulated process that critical for mammalian development. There currently no description of the role long noncoding RNAs (lncRNAs) during pre-implantation stages in goat. The vivo transcriptomes oocytes (n = 3) (n=19) at seven developmental goat were analyzed by RNA sequencing (RNA-Seq). major zygotic gene activation (ZGA) event was found to occur between 8- 16-cell stages. We identified 5,160 differentially expressed lncRNAs...