Plant root exudates affect root-knot nematodes egg hatch. Chemicals in can attract to the roots or result repellence, motility inhibition even death. However, until recently little was known about relationship between tomato chemicals and nematodes. In this study, were extracted from three rootstocks with varying levels of nematode resistance: Baliya (highly resistant, HR), RS2 (moderately MR) L-402 susceptible, T). The effects on Meloidogyne incognita (M. incognita) hatch, survival...

Superior kidding rate is an important economic trait in production of meat goat, and ovulation the precondition rate. MicroRNAs (miRNAs) play critical roles almost all ovarian biological processes, including folliculogenesis, follicle development, atresia, luteal development regression. To find out different activity recruitment with miRNA-mediated posttranscriptional regulation, small RNAs expressed pattern tissues multiple uniparous Anhui White goats during follicular phase was analyzed...

Crosslinking or RNA immunoprecipitation followed by sequencing (CLIP-seq RIP-seq) allows transcriptome-wide discovery of regulatory sites. As CLIP-seq/RIP-seq reads are short, existing computational tools focus on uniquely mapped reads, while to multiple loci discarded. We present CLAM Analysis Multi-mapped reads). uses an expectation–maximization algorithm assign multi-mapped and calls peaks combining reads. To demonstrate the utility CLAM, we applied it a wide range public datasets...

Abstract Lithium‐carbon dioxide batteries (LCBs) require a thick cathode electrode to fulfill their theoretical energy density and high areal capacity (mAh cm −2 ). However, understanding the design of porous electrodes in LCBs is challenging because complexity coupled multispecies transport. Herein, link established between microscopic behaviors macroscopic electrochemical performance through spatio‐temporal resolution technique, filling gap knowledge on degradation mechanism electrodes....

The effects of decreasing dietary crude protein (CP) level on growth performance, nutrient digestion, serum metabolites, and nitrogen utilization in growing goat kids were investigated the current study. Thirty-six male Anhui white randomly assigned to one three CP content diets: 14.8% (control), 13.4%, 12.0% dry matter, respectively. Diets isoenergetic. experiment lasted for 14 weeks, with first two weeks being adaptation. Results showed that low-CP diet decreased average daily gain, feed...

A close association exists among testicular function, gut microbiota regulation, and organismal metabolism. In this study, serum seminal plasma metabolomes, the rumen microbiome of sheep with significant differences in sperm viability, were explored. Serum metabolomes differed significantly between high-motility (HM) low-motility (LM) groups sheep, 39 differential metabolites closely related to motility found while 35 samples. 16S rRNA sequence analysis showed that relative abundance HM LM...

The aim of this study was to determine whether the inclusion 40% common vetch (CV) hay as a feed ingredient in place alfalfa (AH) would improve performance and ruminal fermentation microbiota fattening lambs. Twenty lambs were equally divided into two groups: control group (fed AH with 20% rice straw) CV straw). Concerning quality, had greater vitro digestibility dry matter neutral detergent fiber (

This study aimed to investigate the effects of different dietary concentrate-to-forage ratios on slaughter performance, meat quality, rumen fermentation, microbiota and fecal in Tibetan sheep. A total sixty male sheep were equally allocated into three groups based ratios, i.e., 30:70 (C30), 50:50 (C50), 70:30 (C70). Compared with C30 group, fed C70 diet resulted a higher (

Meniscoplasty is a common surgical procedure used to treat meniscus tears. During the operation, there are often key challenges such as limited visual field, narrow operating space, and difficulties in controlling resection range. Therefore, this study developed an arthroscopic robotic system with ability of autonomous achieve better outcomes. To address issue fields during preoperative intraoperative point cloud images for navigation proposed novel cross-modal registration framework. After...

Background and Purpose Tacrolimus a first‐line medication used after transplantation can induce β‐cell dysfunction, causing new‐onset diabetes mellitus (NODM). Regenerating islet‐derived protein 3 gamma (Reg3g), member of the pancreatic regenerative gene family, has been reported to improve type 1 by promoting regeneration. We aim investigate role Reg3g in reversing tacrolimus‐induced dysfunction NODM mice. Experimental Approach Circulating REG3A (the human homologue mouse Reg3g) heart...

Fresh semen is most commonly used in an artificial insemination of small ruminants, because low fertility rates frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep goats are classified as one species. In order to optimize sperm cryopreservation protocols goat, differences proteomes between ram buck necessary investigate, which may contribute function spermatozoa. the current work, a data-independent acquisition-mass spectrometry proteomic approach...

Introduction This study evaluated the effects of Isatis Leaf (ISL) on growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum urine metabolites, epithelial transcriptome fattening sheep. Methods Twelve 3.5-month-old healthy sheep were randomly divided into two groups, each with 6 replicates, fed basal diet (CON) supplemented 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected histological analysis, fluid feces subjected to...

Sperm proteins play vital roles in improving sperm freezing resilience domestic animals. However, it remains poorly defined which regulate the of spermatozoa rams (Ovis aries). Here, we compared proteome ram with a high cryopreservation recovery ratio (HCR) that low (LCR) using tandem mass tag-based quantitative proteomics approach. Bioinformatic analysis was performed to evaluate differentially expressed (DEPs). A total 2464 were identified, and 184 DEPs screened. Seventy-two higher LCR...

Peroxisome proliferator-activated receptor delta (PPARD) is a key regulator of lipid metabolism, insulin sensitivity, cell proliferation and differentiation. In this study, we identified two Single Nucleotide Polymorphisms (SNPs, g.1015 A>G g.1018 T>C) constituting four haplotypes (GT, GC, AC AT) in the 5’ regulatory region porcine PPARD gene. Functional analysis showed that transcriptional activity promoter fragment carrying haplotype was significantly lower than other 3T3-L1, C2C12 PK-15...

Spermatozoa are unique cells that carry a library of proteins regulate the functions molecules to achieve functional capabilities. Currently, large amounts protein have been identified in spermatozoa from different species using proteomic approaches. However, proteome characteristics and regulatory mechanisms bucks versus rams not fully unraveled. In this study, we performed tandem mass tag (TMT)-labeled quantitative analysis investigate profiles buck (Capra hircus) ram (Ovis aries), two...

The aim of this study was to evaluate the effects three feeding systems, i.e., indoor (CON), with 4-h daily access grazing artificial pasture (ITGP), and 8-h (IEGP), on plasma antioxidant immunological capacity, slaughter characteristics, meat quality economic efficiency Huang-huai lambs.Thirty-three healthy rams similar body weight (approximately 5 mo age, 28.96±1.01 kg) were assigned equally experimental groups. When finished fattening, six lambs from each group collect blood samples for...

Abstract To investigate the effect of Y-27632 on low-temperature metabolism sheep sperm, different concentrations were added to semen at 4 °C in this experiment detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that addition 20 µM significantly increased integrity rate, acrosome capacity, MMP level, adenosine triphosphate (ATP) total cholesterol content, reduced Ca2+...

Mycoplasma ovipneumoniae (M. ovipneumoniae) causes a fatal infection in goats, leading to significant economic losses the small-ruminant industry worldwide. The present study aimed characterize strains of M. infecting goats with pneumonia Anhui Province, China. From November 2021 January 2023, among 20 flocks, total 1320 samples (600 unvaccinated blood, 400 nasal swabs, 200 pleural fluid, and 120 lung tissue) were obtained from typical signs pneumonia, such as low growth rate, appetite...

This study was designed to investigate the accumulation of advanced glycation end-products (AGEs) and expression receptor AGEs (RAGE) in tendinopathic tissues. In this study, posterior tibial tendons (PTT) were collected from patients (n=6). Redundant autografts flexor digitorum longus tendon (FDL; n=3) used for controls. The control tissues extraction proteins western blot sectioned histology immunohistochemistry. Tendinopathy PTT confirmed histologically by presentation disorderly...