A5005999824- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Animal Nutrition and Physiology
- Livestock and Poultry Management
- Livestock Management and Performance Improvement
- Silkworms and Sericulture Research
- Genetic diversity and population structure
- Rangeland Management and Livestock Ecology
- Agronomic Practices and Intercropping Systems
- Livestock Farming and Management
- Agroforestry and silvopastoral systems
- Animal Genetics and Reproduction
- Milk Quality and Mastitis in Dairy Cows
- Crop Yield and Soil Fertility
- Sperm and Testicular Function
- Reproductive biology and impacts on aquatic species
- Plant Growth Enhancement Techniques
- Animal Diversity and Health Studies
- Agricultural pest management studies
- Fish Ecology and Management Studies
- Protein Hydrolysis and Bioactive Peptides
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
Nepal Agricultural Research Council
2015-2024
Chinese Academy of Agricultural Sciences
2015-2020
Institute of Animal Sciences
2020
Ministry of Agriculture and Rural Affairs
2020
Shihezi University
2020
Ningxia Academy of Agriculture and Forestry Sciences
2020
Guangxi University
2020
Tibet Academy of Agricultural and Animal Husbandry Sciences
2020
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
2020
Despite much attention, history of sheep (Ovis aries) evolution, including its dating, demographic trajectory and geographic spread, remains controversial. To address these questions, we generated 45 complete 875 partial mitogenomic sequences, performed a meta-analysis published ovine mitochondrial DNA sequences (n = 3,229) across Eurasia. We inferred that O. orientalis musimon share the most recent female ancestor with aries at approximately 0.790 Ma (95% CI: 0.637–0.934 Ma) during Middle...
Domestic sheep and their wild relatives harbor substantial genetic variants that can form the backbone of molecular breeding, but genome landscapes remain understudied. Here, we present a comprehensive resource for ovine species, landraces improved breeds domestic sheep, comprising high-coverage (∼16.10×) whole genomes 810 samples from 7 species 158 diverse populations. We detected, in total, ∼121.2 million single nucleotide polymorphisms, ∼61 which are novel. Some display significant (P <...
The domestication and subsequent global dispersal of livestock are crucial events in human history, but the migratory episodes during history remain poorly documented [1-3]. Here, we first developed a set 493 novel ovine SNPs male-specific region Y chromosome (MSY) by genome mapping. We then conducted comprehensive genomic analysis chromosome, mitochondrial DNA, whole-genome sequence variations large number 595 rams representing 118 domestic populations across world. detected four different...
Indicine cattle, also referred to as zebu (Bos taurus indicus), play a central role in pastoral communities across wide range of agro-ecosystems, from extremely hot semiarid regions humid tropical regions. However, their adaptive genetic changes following dispersal into East Asia the Indian subcontinent have remained poorly documented. Here, we characterize global diversity using high-quality whole-genome sequencing data 354 indicine cattle 57 breeds/populations, including major...
Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes both swamp river re-sequenced 230 individuals (132 98 buffaloes) sampled from across Asia Europe. Beyond many actionable insights that our study revealed domestication, basic physiology breeding of buffalo, made striking discovery divergent domestication traits between can be explained with recent...
Nepal is a part of the world’s biodiversity hotspot and ranks 49th in world for biodiversity. Agrobiodiversity its conservation status were studied through literature review, field survey, key informant survey focus group discussion. Results implementation some good practices action research also documented. Among 24,300 total species country, 28% are agricultural genetic resources (AGRs), termed as agrobiodiversity. has six components (crops, forages, livestock, aquatic, insects...
Fat tail is a unique trait in sheep acquired during domestication. Several genomic analyses have been conducted breeds from limited geographic origins to identify the genetic factors underlying this trait. Nevertheless, these studies obtained different candidates. The results of regional were easily biased by breed structures.To minimize bias and distinguish true candidates, we used an extended data set 968 representing 18 fat-tailed 14 thin-tailed around world, integrated two statistical...
Abstract Sheep has successfully adapted to the extreme high-altitude Himalayan region. To identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (SNPs) of four major sheep breeds living at different altitudes in Nepal and downloaded SNP array data from additional Asian Middle East breeds. Using a d i value-based genomic comparison between eight lowland breeds, discovered most differentiated variants locus FGF-7 ( Keratinocyte growth factor-7 ),...
Body size, one of the most important quantitative traits under evolutionary scrutiny, varies considerably among species and populations within species. Revealing genetic basis underlying this variation is very important, particularly in humans where there a close relationship with diseases domestic animals as selective patterns are associated improvements production traits. The Debao pony horse breed small body size that unique to China; however, it unknown whether size-related candidate...
Abstract Prolificacy is of key interest in goat ( Capra hircus ). This study aims to understand the genetic basis prolificacy and compare performance kids born single multiple litters. A total 79 Blood EDTA samples were collected from Khari, Boer Crosses. PCR followed by gel-electrophoresis sequencing both directions was done screen for A746G mutation Bone Morphogenetic Protein Receptor 1B (BMPR1B) gene. Farm records July 2018 March 2023 data analyzed using Harvey Mixed Model Computer...
The effect of non-genetic factors on daily milk yield helps optimize production, improve economic efficiency, make informed management decisions, advance breeding programs, and adapt to changing climatic impacts. present study had the main objective assess temperature-humidity index (THI) other including year, season calving, parity, milking, stage lactation (DMY) Jersey Holstein-Friesian dairy herd reared under sub-tropical region at National Cattle Research Center (NCRP), Chitwan, Nepal. A...
This study aimed to evaluate the growth performance of first-generation Boer crossbred kids, focusing on effect non-genetic factors like location, breed, production system, herd size, sex, parity, dam and birth type. The research was carried out at two municipalities Surkhet district namely Birendranagar Lekbesi. Data were gathered from 901 kids 446 does raised in a pasture stall-feeding system. data analyzed using Mixed Model Least-square Maximum Likelihood Computer Program PC-2 statistical...
Nepal borders India in the south and China north. Four distinct indigenous domestic sheep breeds, i.e. Bhyanglung alpine region, Baruwal high hills, Kage mid hills Lampuchhre low lands are distributed country. In this study, mitochondrial DNA control region of 111 from these four breeds was directly sequenced to determine their genetic variations phylogenetic relationships. High diversity among observed all haplotypes were classified into three haplogroups (A C). Among residing middle had...
Objective: Estimating the genetic diversity and structures, both within among chicken breeds, is critical for identification conservation of valuable resources. In chickens, microsatellite (MS) marker polymorphisms have previously been widely used to evaluate these distinctions. Our objective was analyze relationships 22 breeds in Asia based on allelic frequencies.Methods: We 469 genomic DNA samples from eight Asian countries (South Korea, KNG, KNB, KNR, KNW, KNY, KNO; Laos, LYO, LCH, LBB,...
Indigenous buffaloes, as the important livestock species contributing to economy of country, are lifeline livelihood in Nepal. They distributed across diverse geographical regions country and have adapted various feeding, breeding, management conditions. The larger group these native buffalo breeds present narrow stiff hilly terrains. Their dispersal indicates a possible environmental adaptation mechanism, which is crucial for conservation breeds. We utilized whole-genome sequencing (WGS)...
Abstract To identify the genetic determinants of domestication and productivity Asian water buffaloes ( Bubalus bubalis ), 470 genomes domesticated river swamp along with their putative ancestors, wild arnee ) are sequenced integrated. The inherit morphology buffaloes. In contrast, most unique in morphology, but cluster levels genomic diversity Italian Indonesian decrease at opposite extremes distribution range. Purifying selection prevented accumulation harmful loss‐of‐function variants...
Heteroplasmy due to length polymorphism with tandem repeats in mtDNAs within individual was hardly studied domestic animals. In the present study, we identified intra-individual variation control region of Nepalese sheep by molecular cloning and sequencing techniques. We observed one four a 75-bp nucleotide sequences mtDNA 45% total sampled contrast Chinese sheep, indicating that heteroplasmy is specific sheep. The high rate could be resultant mutation independent segregation at level or...
Indigenous chickens are quite popular in Family Poultry Production System (FPPS) Nepal, but constrained with their low productive performance. The present study evaluated the and reproductive performance of Sakini chicken different filial generations sexes. In addition, research also aimed at understanding effect on above performances. Initially, base populations (G-0) were maintained by collecting eight weeks old birds from agro-ecologial zones Nepal. Performance G-0 was based weekly body...
Lulu is an indigenous breed of cattle (Bos taurus) found in high altitude regions western Nepal. Population has been declining due to introgression with other exotic breeds increase milk productivity. Here we aimed at finding potential approach for conserving and its assets by studying the contents investigating which variant beta-casein protein present this breed. Beta caseins are abundant cow A1 A2 being most common genetic variants protein. Consumption type numerous health-related...
Pankaj Kumar Jha, Saroj Sapkota, Neena Amatya Gorkhali, Bhoj Raj Pokharel, Ajeet Shishir Bhandari and Bhola Shankar Shrestha. Journal of Animal Reproduction Biotechnology 2019;34:272-9. https://doi.org/10.12750/JARB.34.4.272
Teosinte (Euchlaena mexicana) is one of the most popular summer growing cereal fodder, rich in energy, dry matter (DM) and other nutrients, yet productivity this fodder Nepal has not been achieved as expected due to several important factors including cultivation management sowing dates. An experiment was done during April August 2018 at Directorate Agricultural Research, NARC, Province-5, Khajura, Banke by using Split Plot design with objective identify appropriate dates relation possible...
This study was carried out to evaluate the quality of raw milk measured by Standard Plate Count (SPC). Individual for Colony Forming Units (CFU) in National Cattle Research Program Rampur Chitwan, Nepal. Milk from Jersey and Holstein cows with two types milking (hand machine milking) collecting three containers (Plastic, aluminum, steel). had different chilling durations (0, 4, 8, 12, 24, 48, 72 hours). Altogether, 252 samples SPC were examined at farm levels. Results showed significant...
This study was conducted to assess the quality and quantity aspects of different genetic group buck’s semen used for Artificial Insemination (AI) at National Livestock Breeding Centre (NLBC), Pokhara. For this, records from breeds groups buck collected over 3 years (2014-2016) were analyzed. Parameters such as volume, density, mass motility, initial motility concentration post thaw analysed various non-genetic factors like year, season, green based feeding. Data analyzed by least square...