A5008189181- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Genetic and phenotypic traits in livestock
- Viral Infections and Vectors
- Zoonotic diseases and public health
- Virology and Viral Diseases
- Parasite Biology and Host Interactions
- Rabies epidemiology and control
- Helminth infection and control
- Livestock and Poultry Management
- Digital Imaging for Blood Diseases
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Vector-borne infectious diseases
- Diagnosis and treatment of tuberculosis
- Livestock Farming and Management
- Parasitic Infections and Diagnostics
- Wildlife Ecology and Conservation
- Effects of Environmental Stressors on Livestock
- Animal Disease Management and Epidemiology
- Parvovirus B19 Infection Studies
- Animal Diversity and Health Studies
- Human-Animal Interaction Studies
- Intestinal and Peritoneal Adhesions
- Toxoplasma gondii Research Studies
National Trust for Nature Conservation
2016-2025
Hokkaido University
2016
NLR Nepal
2016
Tribhuvan University
2013
From wild dogs (Lycaon pictus) in the Serengeti to tigers (Panthera tigris altaica) Russian Far East, canine distemper virus (CDV) has been repeatedly identified as a threat carnivores. Between 2020 and 2022, six Indian leopards (P. pardus fusca) presented Nepali authorities with fatal neurological disease, consistent CDV. Here, we report findings of serosurvey felids from Nepal. A total 48 serum samples were tested, comprising 28 Bengal t. tigris) 20 leopards. Neutralizing antibodies three...
Canine distemper virus (CDV) is responsible for a highly contagious and often fatal neurological disease that affects various carnivores, including domestic dogs. In Nepal, recent reports of CDV exposure illness in leopards (Panthera pardus) have raised concerns about the transmission among dogs wild carnivores. To investigate genetic lineage spread CDV, our study utilized archived post-mortem samples from four exhibited clinical signs suggestive canine infection. These were rescued Palpa,...
Gut microbiome influences host health and well-being. Co-occurring hosts may exchange disease-causing bacteria belonging to these microbial communities. Therefore, monitoring gut microbiota composition in wildlife humans is paramount prevent zoonotic diseases, thus protecting strengthening public health. We characterized diversity abundance of the bacterial component across mahouts (captive elephant trainers handlers), their pachyderms, livestock wild herbivores around Chitwan National Park...
Progesterone is the major reproductive hormone in female elephants to maintain pregnancy and produced by corpus luteum (CL) placenta. This study routinely monitored status of captive Asian (Elephas maximus) Sauraha, Nepal, based on individual blood progesterone profiles. The five were selected from Elephant Breeding Center (EBC) National Trust for Nature Conservation (NTNC). A purposive sampling technique was adopted collect samples at an interval a month 16 months ear vein using vacutainer...
Introduction Tuberculosis is an infectious disease caused by a group of acid-fast bacilli known as Mycobacterium tuberculosis complex (MTC), which has major impact on humans. Transmission MTC across the human-animal interface been demonstrated several studies. However, reverse zoonotic transmission from humans to animals (zooanthroponosis) often neglected. Methods In this study, we used Nanopore MinION and Illumina MiSeq approaches sequence whole genome M. strains isolated two deceased Asian...
Canine distemper virus (CDV) is a global multi-host pathogen that capable of causing considerable mortality in range species and important the field conservation medicine. Nepal’s Chitwan National Park protected area providing habitat for 32% country’s mammal including endangered carnivores such as Bengal tiger (Panthera tigris tigris) are susceptible to CDV. The presence free-roaming dogs around areas could represent source infectious disease transmission local wildlife. A cross-sectional...
INTRODUCTION: Bovine tuberculosis (bTB) is an important public health concern worldwide. This study was conducted to determine the status of bTB in animals raised by patients Western Chitwan, Nepal. MATERIALS AND METHODS: cross-sectional from August, 2011 January, 2012. A total 100 bovines (cattle and buffalo) 60 farms were tested with single intradermal tuberculin test considering various animal factors. Well designed questionnaire survey taken 70 same families focusing knowledge, awareness...
Serum samples of 11 Bengal tigers (Panthera tigris tigris) from Chitwan National Park in Nepal, collected between 2011–17, were evaluated for the presence antibodies to eight diseases commonly investigated large felids. This initial serologic survey was done establish baseline information understand exposure Nepal's free-ranging tiger population these diseases. Tiger serum opportunistically during encounters such as translocation, human conflict, and injury placed cold storage later use....
Knowing the status of disease in domestic animals such as dogs ( Canis lupus familiaris ) fringe areas national parks is important to understand potential prevalance wildlife population. Dog populations bordering pose a risk native wildlife. We carried out seroprevalence study among free-ranging from buffer zone Chitwan National Park (CNP) Nepal. A total 163 were sampled 2017 at two sites CNP and tested for antibodies against common canine pathogens distemper virus (CDV) parvovirus (CPV)...
Abstract Oesophageal obstruction due to hairballs in cats is very rare. Excessive grooming behaviour skin infection can lead impaction the digestive tract, resulting deprivation of food and water ultimately death. This report described a case rescued jungle cat showing signs marked dehydration after On postmortem examination, blockade was observed oesophagus. Further examination hair revealed that from same species, not prey species. suggests over‐grooming over‐ingestion hair, which might...
Abstract To identify the genetic determinants of domestication and productivity Asian water buffaloes ( Bubalus bubalis ), 470 genomes domesticated river swamp along with their putative ancestors, wild arnee ) are sequenced integrated. The inherit morphology buffaloes. In contrast, most unique in morphology, but cluster levels genomic diversity Italian Indonesian decrease at opposite extremes distribution range. Purifying selection prevented accumulation harmful loss‐of‐function variants...
Twelve cat species were recorded in Nepal including the largest, Tiger Panthera tigris, and smallest, Rusty-spotted Cat Prionailurus rubiginosus. There is more research on than small wild cats; consequently, conservation status, distribution, ecology of are poorly known. In this article, we report first photographic evidence Clouded Leopard Neofelis nebulosa Fishing viverrinus Parsa National Park southern central during a camera trap survey targeted at tiger between 2014 2016. only single...
In order to ascertain the comprehensive prevalence of gastrointestinal parasites among captive Asian elephants in Chitwan National Park, a cross-sectional investigation was conducted. A total 103 samples purposefully collected. Demographic details encompassing age and gender, along with epidemiological information concerning deworming status, timing intervals, nutritional condition for both government-owned privately-owned elephants, were procured through structured questionnaire survey. The...
The efficacy evaluation of levamisole and oxyclozanide treatment on the gastrointestinal nematodes ungulates at central zoo, Nepal was carried out from June—August 2021. A total 40 fecal samples were collected 10 species zoo for determining anthelmintic given day 0 pretreatment post-treatment analysis 07 14. concentration method (floatation concentration) used microscopic examination eggs, quantitative (EPG) nematode eggs with help modified McMaster slides. identification done using an optic...
The greater one-horned rhinoceros (Rhinoceros unicornis) is listed as vulnerable by the IUCN Red List. Mycobacterium orygis-associated disease was identified in a single rhino Chitwan National Park February 2015 prior to planned translocation of five from Bardia for conservation purposes. This paper describes qualitative risk analysis conducted retrospectively post-translocation orygis and this translocation, with aim improve understanding threats rhino. method used devised Sainsbury &...
Koshi Tappu Wildlife Reserve (KTWR) has the last remaining Nepalese population of Endangered Asiatic Wild Buffalo (Bubalus arnee Kerr, 1792). Individual animals protected inside KTWR may be purely wild, domestic or hybrid origin, and wild is under potential threat due to habitat loss genetic introgression from feral backcrosses. Identification genetically pure individuals important for identifying translocation other areas within their former range. In this study we have sequenced a highly...
Tuberculosis (TB) is an infectious zoonotic disease, characterized by the development of tubercles resulting in caseation and calcification lungs. In elephants, causative agents Mycobacterium tuberculosis bovis result chronic weight loss, anorexia, weakness with occasional dyspnea or coughing. TB has been a major threat to elephants Nepal. To date, 17 have died Nepal due alone. Therefore, this study was undertaken screen seropositive A cross-sectional conducted determine seroprevalence...
Tuberculosis (TB) is an emerging threat to the survival of elephants in Nepal. We investigated lung tissue samples from nine that died 2019 2022 Nepal using culture, conventional PCR, and loop-mediated isothermal amplification (LAMP) then performed genotyping five PCR-positive isolates understand possible transmission dynamics Mycobacterium tuberculosis (Mtb). Results showed two-thirds (6/9) were confirmed be infected Mtb by LAMP, 5/9 4/9 culture. Genotyping with Indo-Oceanic Beijing...
A study was conducted to estimate the hematological values of captive white-rumped vultures (Gyps bengalensis) in Nepal. Blood samples were collected from 23 adults and 14 juvenile during their annual health examinations at Vulture Conservation Breeding Center, Kasara, Chitwan, Of adults, 12 (52%) male 11 (48%) females, whereas sex juveniles undifferentiated. The mean (± SD) for adult birds estimated as red blood cell count (2.86 ± 1.01 × 106/µL), white (14.75 6.01 103/µL, hemoglobin...