A5061448387- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Milk Quality and Mastitis in Dairy Cows
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Genomics and Phylogenetic Studies
- Fatty Acid Research and Health
- Livestock and Poultry Management
- Digestive system and related health
- Animal Nutrition and Physiology
- Adipose Tissue and Metabolism
- Cholesterol and Lipid Metabolism
- Livestock Farming and Management
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Genetic diversity and population structure
- Infant Nutrition and Health
- RNA Research and Splicing
- Bone Metabolism and Diseases
- Cytokine Signaling Pathways and Interactions
- TGF-β signaling in diseases
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Protein Hydrolysis and Bioactive Peptides
Yunnan Agricultural University
2015-2025
Yunnan University
2005-2010
Wuhan University
2009
Much like other indigenous domesticated animals, Tibetan chickens living at high altitudes (2,200–4,100 m) show specific physiological adaptations to the extreme environmental conditions of Plateau, but genetic bases these are not well characterized. Here, we assembled a de novo genome chicken and resequenced whole genomes 32 additional chickens, including village game fowl, Red Junglefowl, found that could broadly be placed into two groups. Further analyses revealed several candidate genes...
Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes both swamp river re-sequenced 230 individuals (132 98 buffaloes) sampled from across Asia Europe. Beyond many actionable insights that our study revealed domestication, basic physiology breeding of buffalo, made striking discovery divergent domestication traits between can be explained with recent...
Abstract The newly sequenced mitochondrial genomes of 107 Asian swamp buffalo ( Bubalus bubalis carabensis ) allowed the reconstruction matrilineal divergence since ~900 Kya. Phylogenetic trees and Bayesian skyline plots suggest a role glacial periods in demographic history buffalo. ancestral swamp-buffalo mitogenome is dated ~232 ± 35 Two major macro-lineages diverged during 2 nd Pleistocene Glacial Period (~200–130 Kya), but most (~99%) current matrilines derive from only two ancestors...
Abstract Studies on 3T3-L1 cells and HepG2 hepatocytes have shown that phosphatidic acid phosphohydrolase1 (LPIN1) plays a key role in adipogenesis, acting as co-activator of peroxisome proliferator-activated receptor gamma coactivator 1a (PGC-1a) to regulate fatty metabolism. However, the functional regulatory mechanism LPIN1 gene milk fat synthesis buffalo are still unknown. In this study, overexpression transfected with recombinant fusion expression vector significantly increased AGPAT6 ,...
Background/Objectives: Sterol regulatory element-binding transcription factor 2 (SREBF2) is a key involved in regulating cholesterol homeostasis. However, its role buffalo mammary gland lipid metabolism remains unclear. Methods: To address this, we isolated and characterized the SREBF2 gene from glands performed an in-depth analysis of molecular characteristics, tissue-specific expression, functional roles epithelial cells (BuMECs). Additionally, investigated single nucleotide polymorphisms...
Abstract Background In contrast to most animal genomes, mitochondrial genomes in species belonging the phylum Cnidaria show distinct variations genome structure, including mtDNA structure (linear or circular) and presence absence of introns protein-coding genes. Therefore, analysis nuclear insertions sequences (NUMTs) cnidarians allows us compare NUMT content animals with different structures. Results identification Hydra magnipapillata , Nematostella vectensis Acropora digitifera showed...
Abstract We hypothesized that insulin-induced gene 1 ( INSIG1 ) affects milk fat synthesis in buffalo. For this reason, the protein abundance of mammary tissue buffalo during peak period lactation and dry-off was evaluated. The results showed expression at lower than period. To explore role synthesis, epithelial cells BMECs were isolated purified from tissue, overexpressed knocked down by constructing recombinant lentivirus vector transfecting into BMECs. Results revealed overexpression...
Recent studies have shown elongase of very-long-chain fatty acids 6 (ELOVL6) is a vital protein for endogenous synthesis saturated and monounsaturated long-chain in some mammals. Nevertheless, its role lipid buffalo mammary gland still unclear. In this work, the full-length coding sequence (CDS) ELOVL6 was cloned identified from gland. As result, CDS gene 795 bp, which encodes polypeptide 264 amino acid residues. The contains an ELO domain belongs to superfamily. Among 10 tissues peak...
AGPAT6 plays a crucial role in the triglyceride (TG) synthesis pathway mammals. However, its roles buffalo lactation remain unknown. Therefore, we investigated functional of milk fat by transfecting overexpression and lentivirus interference vectors mammary epithelial cells (BuMECs) vitro. BuMECs significantly enhanced mRNA expression FABP4, SLC27A6, ACSL1, DGAT1, DGAT2, LPIN1, INSIG1, CEBPA SREBF1 genes, reduced that XDH, CPT1A, LIPE, INSIG2 PPARGC1A, but has no significant influence to...
Abstract. TP53 has been shown to be involved in lactation cattle. However, the role of buffalo remains unknown. To this end, we isolated and identified complete coding sequence (CDS) gene from mammary gland further analyzed its molecular characteristics, function, tissue differential expression, single-nucleotide polymorphism (SNP). A transcript was cloned with a CDS length 1161 bp, encoding protein consisting 386 amino acid residues. Bioinformatics analysis showed that physicochemical...
with Red Junglefowl harboring most microsatellite alleles (23).High levels of heterozygosity were observed within populations indicated by a mean unbiased H E value 0.663, which was higher than the reported for elsewhere.The F IS domestic ranged from -0.098-0.005,indicating lack inbreeding among these populations.A high proportion significant departures (89) 224 HWE tests each locus in population reflected an excess and substructure.Individual assignment tests, ST values (0.1757-0.3015),...
Abstract Peroxisome proliferator‐activated receptor gamma (PPARγ) is a critical transcription factor regulating lipid and glucose metabolism. However, the regulatory effect of PPARγ on milk fat synthesis in buffalo mammary gland not clear. In order to explore role PPARG gene synthesis, lentivirus‐mediated interference was used knock it down then recombinant fusion expression vector transfected into epithelial cell (BMEC) overexpress it. knockdown significantly decreased CD36 , FABP3 FABP4...
Testis-specific serine kinases (TSSKs) are a family of serine/threonine highly expressed in the testes that responsible for regulating many spermatogenesis-related protein activities. Mutations this have positive relationship with oligospermia and azoospermia human mouse. Here, five members TSSK from Banna mini-pig inbred line (BMI) were cloned, sequenced, characterized. The full-length coding sequences BMI TSSKs varied 807 (TSSK3) to 1095 bp (TSSK1) encoded 268 364 amino acids molecular...
Domestication of the helmeted guinea fowl (HGF; Numida meleagris) in Africa remains elusive. Here we report a high-quality de novo genome assembly for domestic HGF generated by long- and short-reads sequencing together with optical chromatin interaction mapping. Using this as reference, performed population genomic analyses newly sequenced whole-genomes 129 birds from Africa, Asia, Europe, including animals (n = 89), wild progenitors 34), their closely related species 6). Our results reveal...
Characterization of a novel water buffalo gene, AGPAT6 in the cytoplasm.The deduced amino acid sequences share 99, 98, 97, 97 and 95% identity with their homologous from cattle, horse, human, mouse, orangutan, pig, rat, chicken, respectively.The phylogenetic tree analysis based on CDS showed that has closer genetic relationship cattle than other species.Tissue expression profile shows this gene is highly expressed mammary gland, moderately heart, muscle, liver, brain; weakly pituitary...
Abstract. PPARGC1A exerts important functions in activating many nuclear receptors and transcription factors that are related to energy balance. Previous studies have shown gene is associated with lactation traits of dairy cattle. However, the functional role buffalo still unknown. In this work, complete coding sequence (CDS) was isolated characterized for swamp river buffalo. The CDS length both types same, which composed 2394 nucleotides encoded a peptide 797 amino acid residues. This...
Leptin (LEP), a protein hormone well-known for its role in metabolic regulation, has recently been linked to lipid metabolism cattle. However, function buffalo mammary glands remains unclear. To address this issue, we isolated and identified the LEP gene conducted experiments investigate epithelial cells (BuMECs). In study, two transcript variants of LEP, designated as LEP_X1 LEP_X2, were identified. The coding sequences (CDS) LEP_X2 are 504 bp 579 length, encoding 167 192 amino acid...
The SCD is a rate-limiting enzyme that catalyzes the synthesis of monounsaturated fatty acids (MUFAs) in dairy cows; however, its role mammary gland buffalo not well understood. In this study, we isolated and characterized complete coding sequence (CDS)
Kappa casein plays a crucial role in the formation of stable micelles and has key influence on milk-clotting properties. However, current understanding buffalo CSN3 gene polymorphisms is not sufficient. In this study, complete coding sequence (CDS) were detected using PCR product direct sequencing. The CDS for river swamp was same length, which contained an open reading frame 573 nucleotides encoding peptide containing 190 amino acid residues. A total eight single nucleotide (SNPs)...