A5079251282- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Sperm and Testicular Function
- Immunotherapy and Immune Responses
- Reproductive Biology and Fertility
- FOXO transcription factor regulation
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Ultrasound and Hyperthermia Applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Viral gastroenteritis research and epidemiology
- Tissue Engineering and Regenerative Medicine
- Birth, Development, and Health
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Sexual Differentiation and Disorders
- Neuroscience and Neural Engineering
- GDF15 and Related Biomarkers
- Immune Response and Inflammation
- Cell Image Analysis Techniques
- Heat shock proteins research
- Aquaculture disease management and microbiota
- Retinal Development and Disorders
Biogen (United States)
2010-2024
University of Hawaiʻi at Mānoa
2013-2024
University of Hawaii System
2008-2023
Pacific Biosciences (United States)
2009-2020
Institute of Physiology and Basic Medicine
2015
Genesis Research Institute
2015
University of California, Davis
2013
North Carolina State University
2009
Walker (United States)
2006
A nonviral vector for highly efficient site-specific integration would be desirable many applications in transgenesis, including gene therapy. In this study we directly compared the genomic efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity all lines whereas had no activity. Furthermore, transposase coupled to GAL4 DNA-binding domain retains similarly manipulated products...
The G allele of the FOXO3 single nucleotide polymorphism (SNP) rs2802292 exhibits a consistently replicated genetic association with longevity in multiple populations worldwide. aims this study were to quantify mortality risk for longevity-associated genotype and discover particular cause(s) death associated older Americans diverse ancestry. It involved 17-year prospective cohort 3584 American men Japanese ancestry from Honolulu Heart Program cohort, followed by replication 1595 white 1056...
Journal Article Transcription activator like effector (TALE)-directed piggyBac transposition in human cells Get access Jesse B. Owens, Owens 1Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School Medicine, University Hawaii at Manoa, Honolulu, HI 96822, USA, 2Genome Center, Molecular California, Davis, CA 95616, USA 3Manoa BioSciences, 96819, Search other works by this author on: Oxford Academic PubMed Google Scholar Damiano Mauro, Mauro...
Efficient integration of functional genes is an essential prerequisite for successful gene delivery such as cell transfection, animal transgenesis, and therapy. Gene strategies based on viral vectors are currently the most efficient. However, limited cargo capacity, host immune response, risk insertional mutagenesis limiting factors concern. Recently, several groups have used transposon-based approaches to deliver a variety cells. The piggyBac (pB) transposase in particular has been shown be...
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert large transgene into genome. This procedure increased integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated Our data indicate that additionally requires fewer embryos be microinjected than traditional methods obtain transgenic animals....
Integrating vectors such as viruses and transposons insert transgenes semi-randomly can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location insertion is required. The piggyBac (PB) transposase an efficient gene transfer vector active in variety cell types proven amenable modification. Here we present design validation chimeric PB proteins fused Gal4 DNA binding domain with ability target pre-determined sites....
The process of transgenesis involves the introduction a foreign gene, transgene, into genome an animal. Gene transfer by pronuclear microinjection (PNI) is predominant method used to produce transgenic animals. However, this technique does not always result in germline offspring and has low success rate for livestock. Alternate approaches, such as somatic cell nuclear using fibroblasts, do show increase efficiency compared PNI, while viral-based hampered issues regarding transgene size...
The efficiency of intracytoplasmic sperm injection (ICSI)-mediated transgenesis is often limited by poor embryo development. Because our previous work indicated that impairment development frequently related to chromosomal abnormalities, we hypothesized foreign DNA and/or conditions used enhance integration the might induce chromosome damage. Therefore, examined chromosomes mouse embryos produced with EGFP gene. Spermatozoa were processed three methods cause membrane disruption:...
Significance The sex-determining factor SRY is thought to function by up-regulating expression of its key target gene SRY-box 9 ( SOX9 ) in pre-Sertoli cells the developing gonads, but evidence for a transactivation domain lacking human and limited vitro mouse Sry. latter unusual possessing polyglutamine tract at C terminus. We demonstrate, using combination biochemical, cell-based, transgenic assays, that this plays essential roles both protein stabilization Sox9 , required male sex...
T cell activation triggers metabolic reprogramming to meet increased demands for energy and metabolites required cellular proliferation. Ethanolamine phospholipid synthesis has emerged as a regulator of shifts in stem cells cancer cells, which led us investigate its potential role during activation. As selenoprotein I (SELENOI) is an enzyme participating two pathways the phosphatidylethanolamine (PE) plasmenyl PE, we generated SELENOI-deficient mouse models determine loss-of-function effects...
A low molecular mass (18 kD) phosphoprotein (pp18) was characterized and purified from cultured sugarcane (Saccharum officinarum L.) cell line H50–7209. Autophosphorylation assays were used to detect pp18 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Only detected a brief in situ phosphorylation method, whereas additional putative protein kinases an extended method. present both microsomal membrane soluble fractions exhibited anomalous turnover of...
Abstract Transgenic mice are vital tools in both basic and applied research. Unfortunately, the transgenesis process as well many other assisted reproductive techniques involving embryo transfer rely on vasectomized males to induce pseudopregnancy surrogate mothers. Vasectomy is a surgical procedure associated with moderate pain must be carried out under full anaesthesia by qualified personnel. Eliminating need for vasectomy would beneficial from economic animal welfare point of view. Our...
We recently reported that protection against coronary artery disease (CAD) mortality is the major contributor to longer life associated with FOXO3 genotype. The present study examined this relation in more detail. performed a 15-year observational of 3,584 older American men Japanese ancestry from Kuakini Honolulu Heart Program cohort and 1,595 White 1,067 Black elderly individuals Health Aging Body Composition study. Multivariate Cox regression models demonstrated carriage...
We have developed a novel method for mouse transgenesis. The procedure relies on hyperactive Tn5 transposase to insert transgene into chromosomes during intracytoplasmic sperm injection. This integrates foreign DNA the genome with dramatically increased effectiveness as compared conventional methods such pronuclear microinjection and traditional injection-mediated Our data indicate that this method, transgenic mice, both hybrids inbreds, can be produced more consistently lower numbers of...
Growth differentiation factor 11 (GDF11) is one of the significant genes that control skeletal formation. Knockout GDF11 function causes abnormal patterning anterior/posterior axial skeleton. The mRNA initially translated to a precursor protein undergoes proteolytic cleavage generate C-terminal peptide or mature GDF11, and N-terminal named propeptide. propeptide can antagonize activity in vitro. To investigate effects on vivo, we generated transgenic mice over-express cDNA tissue. showed...
Abstract We have developed a mouse transgenesis technique that facilitates the insertion of large (∼200 kilo base pairs) DNA fragments into host genomes both inbred and hybrid mice. Six three transgenic mice carrying single bacterial artificial chromosome (BAC) clone with genes located in Down syndrome critical region human 21 were produced using this technology. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.
TRAIL and IFNγ are promising anti-cancer cytokines it has been shown that may sensitize cancer cells to TRAIL. Adipose derived mesenchymal stem (ADSCs) attractive vehicles for delivering agents. In this study, we evaluated the therapeutic potential of PhiC31 (φC31) recombinase and/or piggyBac transposase (pBt) modified ADSCs expressing either TRAIL, IFNγ, or co-expressing TRAIL/IFNγ in mouse models melanoma. The expression bioactivity φC31 pBt were confirmed. We examined effects on signal...