A5023091772- Animal Disease Management and Epidemiology
- Cancer-related molecular mechanisms research
- Vector-Borne Animal Diseases
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Pancreatic and Hepatic Oncology Research
- Viral gastroenteritis research and epidemiology
- Glioma Diagnosis and Treatment
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Carbon and Quantum Dots Applications
- Plant Pathogens and Fungal Diseases
- Cancer Cells and Metastasis
- Fungal Infections and Studies
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Chromatography in Natural Products
- Epigenetics and DNA Methylation
- Thyroid Cancer Diagnosis and Treatment
- Animal Genetics and Reproduction
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Colorectal Cancer Surgical Treatments
- RNA Research and Splicing
Huazhong University of Science and Technology
2024
Tongji Hospital
2024
Central People's Hospital of Zhanjiang
2024
Beijing Chao-Yang Hospital
2024
Capital Medical University
2021-2024
New Hope Liuhe (China)
2021-2024
Taiyuan University of Technology
2024
Second Military Medical University
2000-2023
Ministry of Agriculture and Rural Affairs
2023
Shanghai Changzheng Hospital
2013-2023
This work designed a novel platform for effective sensing of biomolecules by fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to graphene oxide (GO). The QDs were first modified with molecular beacon (MB) as probe recognize the target analyte. strong interaction between MB and GO led fluorescent quenching QDs. Upon recognition target, distance increased, target-bound became weaker, which significantly hindered FRET and, thus, increased change in intensity produced method...
The human genome encodes many long non-coding RNAs (lncRNAs). However, their biological functions, molecular mechanisms, and the prognostic value associated with pancreatic ductal adenocarcinoma (PDAC) remain to be elucidated. Here, we identify a fundamental role for lncRNA HOXA transcript at distal tip (HOTTIP) in progression chemoresistance of PDAC.High-throughput microarrays were performed detect expression profiles lncRNAs messenger eight PDAC tissues four tissues. Quantitative real-time...
PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and involved maintaining genome integrity by silencing transposons. These also DNA/histone methylation gene expression regulation somatic of invertebrates. The functions piRNAs vertebrates, however, remain elusive. We found that snoRNA-derived C (C′)/D′ (D)-box conserved abundant human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to...
Pancreatic ductal adenocarcinoma (PDAC) is still a lethal malignancy. Long noncoding RNAs (lncRNAs) have been shown to play critical role in cancer development and progression. Here we identified overexpression of the lncRNA AFAP1-AS1 PDAC patients evaluated its prognostic functional relevance. The global expression profile was measured by microarray. Expression reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) 90 tissue samples adjacent normal tissues. impact on cell...
// Yingxue Wang 1, * , Zhihua Li 2, Shangyou Zheng Yu Zhou 1 Lei Zhao 3 Huilin Ye Xiaohui 4 Wenchao Gao Zhiqiang Fu Quanbo Yimin Liu Rufu Chen Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department Hepatopancreatobiliary Surgery, Sun Yat-sen Memorial Hospital, University, Guangzhou, China 510120 2 Medical Oncology, Radiation Yat-Sen University Cancer Center, 510060 These authors have contributed equally to this work Correspondence to: Chen, e-mail:...
A novel biosensing strategy for selective electrochemical detection of DNA down to the attomolar level with a linear range 5 orders magnitude was developed by specific recognitions target and streptavidin biotin labelled molecular beacon signal amplification streptavidin–horseradish peroxidase functionalized carbon nanotubes.
Pancreatic ductal adenocarcinoma (PDAC) cells utilize a novel non-canonical pathway of glutamine metabolism that is essential for tumor growth and redox balance. Inhibition this metabolic in PDAC can potentially synergize with therapies increase intracellular reactive oxygen species (ROS) such as radiation. Here, we evaluated the dependence pancreatic cancer stem (PCSCs) on researched whether inhibiting enhance radiosensitivity PCSCs. We showed deprivation significantly inhibited...
MicroRNAs (miRNAs) play a prominent role in gastric cancer (GC) initiation and progression. In this study, we found that miR‐374a expression was up‐regulated human GC cell lines tissues. Inhibition of suppressed proliferation, migration invasion vitro slowed tumor growth vivo. SRC kinase signaling inhibitor 1 (SRCIN1) identified as direct target miR‐374a. Silencing SRCIN1 significantly enhanced invasion, whereas reintroduction partially abrogated the oncogenic effects Taken together, these...
Pancreatic ductal adenocarcinoma (PDAC) is seriously resistant to radiotherapy and the mechanism largely unknown. HOX transcript antisense intergenic RNA (HOTAIR) overexpressed in PDAC. However, function of HOTAIR has never been related radiosensitivity In this present study, expression PDAC cell lines tissues was measured by quantitative real-time PCR (qRT-PCR), association between levels X-ray treatment investigated. Additionally, influence knockdown on radiosensitivity, proliferation,...
Highlights•Sirt5+ subset in wild-type Kras colorectal tumors determines multidrug resistance•Sirt5-mediated demalonylation and inactivation of SDHA lead to succinate accumulation•Succinate binds activates TrxR2 maintain chemotherapy resistance•Succinate inhibits aKG-dependent dioxygenases regulate cetuximab resistanceSummaryA major obstacle for successful management patients with carcinoma (CRC) is resistance anti-cancer cytotoxic treatments. Here, we identified a mechanism CRCs based on the...
Cellular prion protein (PrPc) is a glycosylphosphatidylinositol-anchored membrane that has various physical functions, including protection against apoptotic and oxidative stress, cellular uptake of copper ions, transmembrane signaling, adhesion to the extracellular matrix. In this study, we show PrPc highly expressed in colorectal adenocarcinomas. Transcriptome profiling PrPc-depleted DLD-1 cells revealed downregulation glucose transporter 1 (Glut1). shown be involved regulating Glut1...
The process of transgenesis involves the introduction a foreign gene, transgene, into genome an animal. Gene transfer by pronuclear microinjection (PNI) is predominant method used to produce transgenic animals. However, this technique does not always result in germline offspring and has low success rate for livestock. Alternate approaches, such as somatic cell nuclear using fibroblasts, do show increase efficiency compared PNI, while viral-based hampered issues regarding transgene size...
Introduction The aim of present study was to profile the glucose-dependent and glutamine- dependent metabolism in pancreatic cancer. Methods We performed Immunohistochemical staining GLUT1, CAIX, BNIP3, p62, LC3, GLUD1, GOT1. Based on expression metabolism-related proteins, metabolic phenotypes tumors were classified into two categories, including glucose- glutamine-dependent metabolism. There Warburg type, reverse mixed null type metabolism, canonical non-canonical Results Longer overall...
Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing personalized therapy. In this study, we successfully identified detectable levels tumor-derived cell-free DNA (cfDNA) cerebrospinal fluid (CSF) samples with MB. Furthermore, cfDNA from CSF can interrogate tumor-associated molecular clues. MB-associated alterations CSF, tumor, and post-chemotherapy...
Multidrug resistance (MDR) is one of the major reasons for failure liver cancer chemotherapy, and its suppression may increase efficacy chemotherapy. NANOG plays a key role in regulation embryonic stem cell self-renewal pluripotency. Recent studies reported that was abnormally expressed several types tumors, indicating related to tumor development. However, correlation between chemoresistance remains uncertain. In this study, RNA interfere technology employed knock down expression HepG2...
Sporothrix globosa is a thermo-dimorphic fungus belonging to pathogenic clade that also includes schenckii, which causes human and animal sporotrichosis. Here, we present the first genome assemblies of two S. strains providing data for future comparative genomic studies in species.