A5100319846- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Lung Cancer Diagnosis and Treatment
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- RNA and protein synthesis mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Tracheal and airway disorders
- Extracellular vesicles in disease
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Esophageal Cancer Research and Treatment
- Ferroptosis and cancer prognosis
- Protein Tyrosine Phosphatases
- Fibroblast Growth Factor Research
- Animal Disease Management and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
Nanchang University
2017-2025
Jiangsu Province Hospital
2018-2025
Nanjing Medical University
2016-2025
Jiangxi Provincial People's Hospital
2025
Third Hospital of Nanchang
2013-2024
Institute of Microbiology
2021-2024
Chinese Academy of Sciences
2004-2024
Microbiology Institute of Shaanxi
2021-2024
University of Chinese Academy of Sciences
2021-2024
Czech Academy of Sciences, Institute of Microbiology
2024
Deep sequencing studies frequently identify small RNA fragments of abundant RNAs. These are thought to represent degradation products their precursors. Using sequencing, computational analysis, and sensitive northern blot assays, we show that constitutively expressed non-coding RNAs such as tRNAs, snoRNAs, rRNAs snRNAs preferentially produce 5′ 3′ end fragments. Similar microRNA processing, these terminal generated in an asymmetric manner predominantly favors either the or end....
The continuing discoveries of potentially active small RNAs at an unprecedented rate using high-throughput sequencing have raised the need for methods that can reliably detect and quantitate expression levels RNAs. Currently, northern blot is most widely used method validating are identified by such as sequencing. We describe a new blot-based protocol (LED) RNA (approximately 15-40 bases) detection digoxigenin (DIG)-labeled oligonucleotide probes containing locked nucleic acids (LNA)...
The human genome encodes many long non-coding RNAs (lncRNAs). However, their biological functions, molecular mechanisms, and the prognostic value associated with pancreatic ductal adenocarcinoma (PDAC) remain to be elucidated. Here, we identify a fundamental role for lncRNA HOXA transcript at distal tip (HOTTIP) in progression chemoresistance of PDAC.High-throughput microarrays were performed detect expression profiles lncRNAs messenger eight PDAC tissues four tissues. Quantitative real-time...
Background Probabilistic functional gene networks are powerful theoretical frameworks for integrating heterogeneous genomics and proteomics data into objective models of cellular systems. Such provide syntheses millions discrete experimental observations, spanning DNA microarray experiments, physical protein interactions, genetic comparative genomics; the resulting can then be easily applied to generate testable hypotheses regarding specific functions associations. Methodology/Principal...
Gene networks are an efficient route for associating candidate genes with biological processes. Here, used to discover more than 15 new ribosomal subunit maturation, rRNA processing, and export from the nucleus.
A facile and efficient method for the precise editing of large viral genomes is required selection attenuated vaccine strains construction gene therapy vectors. The type II prokaryotic CRISPR-Cas (clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)) RNA-guided nuclease system can be introduced into host cells during replication. CRISPR-Cas9 robustly stimulates targeted double-stranded breaks in DNA viruses, where non-homologous end joining (NHEJ)...
We present a comprehensive map of over 1 million polyadenylation sites and quantify their usage in major cancers tumor cell lines using direct RNA sequencing. built the Expression Polyadenylation Database to enable visualization maps various facilitate discovery novel genes gene isoforms that are potentially important tumorigenesis. Analyses indicate large fraction (∼30%) mRNAs contain alternative 3' untranslated regions, independent type. The shortest region preferentially upregulated...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal malignancy. Long non‐coding RNAs (lncRNAs) are important regulators in pathological processes, yet their potential roles PDAC poorly understood. Here, we identify fundamental role for novel lincRNA, linc00511, the progression of PDAC. Linc00511 levels tissue specimens and cell lines were examined by quantitative real‐time PCR. Corresponding adjacent non‐neoplastic tissues used as controls. The function linc00511 was determined RNA...
// Yingxue Wang 1, * , Zhihua Li 2, Shangyou Zheng Yu Zhou 1 Lei Zhao 3 Huilin Ye Xiaohui 4 Wenchao Gao Zhiqiang Fu Quanbo Yimin Liu Rufu Chen Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department Hepatopancreatobiliary Surgery, Sun Yat-sen Memorial Hospital, University, Guangzhou, China 510120 2 Medical Oncology, Radiation Yat-Sen University Cancer Center, 510060 These authors have contributed equally to this work Correspondence to: Chen, e-mail:...
Metastasis is the main cause of death in patients with advanced lung cancer. The exosomes released by cancer cells create tumor microenvironment, and then accelerate metastasis. Cancer-derived are considered to be driving force for metastasis niche formation at foreign sites, but mechanism Non-small cell carcinoma (NSCLC) unclear. In metastatic NSCLC patients, expression level miR-3157-3p circulating was significantly higher than that non-metastatic patients. Here, we found can transferred...
The brown planthopper (Nilaparvata lugens Stål; BPH), one of the most destructive pests rice, has proven to be a substantial threat, conferring enormous production losses in Asia and becoming difficult challenge manipulate control under field conditions. continuous use insecticides promotes resurgence BPH, which results resistant varieties adapting through upgrading new BPH biotypes. To overcome resistance acquired by against varieties, different forms novel gene fusions act as functional...
Lymph node (LN) metastasis occurs frequently in pancreatic ductal adenocarcinoma (PDAC) and predicts poor prognosis for patients. The KRASG12D mutation confers an aggressive PDAC phenotype that is susceptible to lymphatic dissemination. However, the regulatory mechanism underlying mutation-driven LN remains unclear. Herein, we found with (KRASG12D PDAC) sustained extracellular vesicle-mediated (EV-mediated) transmission of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) a...
BUD23 was identified from a bioinformatics analysis of Saccharomyces cerevisiae genes involved in ribosome biogenesis. Deletion leads to severely impaired growth, reduced levels the small (40S) ribosomal subunit, and block processing 20S rRNA 18S rRNA, late step 40S maturation. Bud23 belongs S-adenosylmethionine-dependent Rossmann-fold methyltransferase superfamily is related small-molecule methyltransferases. Nevertheless, we considered that methylates rRNA. Methylation G1575 only mapped...
Hepatitis C Virus core protein (HCVc) plays important roles in the development of intrahepatic cholangiocarcinoma (ICC). MicroRNAs (miRNAs) contribute to tumor progression by interacting with downstream target genes. However, regulation and role miRNAs HCV‐related (HCV‐ICC) is poorly understood. In this study, we found that miR‐124 was down‐regulated HCV‐ICC induction DNMT1 HCVc mediated suppression miR‐124. Over‐expression suppressed cell migration invasion vitro, reduced levels SMYD3 genes...
Myelination of the central nervous system (CNS) is critical to vertebrate systems for efficient neural signaling. CNS myelination occurs as oligodendrocytes terminally differentiate, a process regulated in part by myelin regulatory factor, MYRF. Using bioinformatics and extensive biochemical functional assays, we find that MYRF generated an integral membrane protein must be processed release its transcription factor domain from membrane. In contrast most membrane-bound factors, proteolysis...
Purpose. The Src family kinase Fyn, heterogenous nuclear ribonucleoprotein (HnRNP) A2B1 and Sam68 are thought to be associated with the metastasis of tumors, but their roles in regulation apoptosis remain unclear. This study investigated role Fyn its potential relationship HnRNPA2B1 pancreatic cancer. Experimental design. We examined both activity expression human cancer tissues systematically apoptotic mechanisms induced by using multiple experimental approaches. Results. found that was...
Pancreatic ductal adenocarcinoma (PDAC) is seriously resistant to radiotherapy and the mechanism largely unknown. HOX transcript antisense intergenic RNA (HOTAIR) overexpressed in PDAC. However, function of HOTAIR has never been related radiosensitivity In this present study, expression PDAC cell lines tissues was measured by quantitative real-time PCR (qRT-PCR), association between levels X-ray treatment investigated. Additionally, influence knockdown on radiosensitivity, proliferation,...